Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,273,080 C→T 8.5% intergenic (+45/+246) PP_2003 → / ← PP_2004 hypothetical protein/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,273,0800CT 8.5% 125.0 / 2.6 47intergenic (+45/+246)PP_2003/PP_2004hypothetical protein/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (21/22);  new base T (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01

AGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAATGAGCAAA  >  NC_002947/2272937‑2273228
                                                                                                                                                 |                                                                                                                                                    
aGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGt                                                                                                                                                  >  3:54680/1‑148 (MQ=255)
  ccAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCa                                                                                                                                               <  4:54680/149‑1 (MQ=255)
   cAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAg                                                                                                                                              <  6:290284/149‑1 (MQ=255)
           gCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCTTCCCTTTGATGGGTCGCCTCGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTaa                                                                                                                                       <  1:85845/149‑1 (MQ=255)
           gCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTaa                                                                                                                                       >  6:147532/1‑148 (MQ=255)
                   tgGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATcccc                                                                                                                               <  8:223260/148‑1 (MQ=255)
                      ccccGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGtt                                                                                                                           <  8:7401/149‑1 (MQ=255)
                      ccccGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGt                                                                                                                            >  4:281532/1‑148 (MQ=255)
                       cccGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCCACCCATTCCCCGGCCGCCCCCGAAAGGGCCGGTTCAGGGGGTAAAAAACCCCTGtt                                                                                                                           >  4:118171/1‑148 (MQ=255)
                            cggccgAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGAc                                                                                                                     <  3:238405/149‑1 (MQ=255)
                                    gCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCcgcg                                                                                                             <  5:147532/149‑1 (MQ=255)
                                             aTTCAGGTGCAGCAGGATGCCCAGCCGTCC‑GAGGCCGACGCCACCTCCCTTTGATGGGTAGCAG‑GAACCCACCCATTATCCAGCAACCCGAGAAATGGCCGGTAAAGGGTGTAAACATACCCTGTTTAACAAGCCCAGCGaaaaaaata                                                                                                    >  8:291049/1‑144 (MQ=255)
                                              ttCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATACCCAGTTACAGACGCCCCGCGATAACTTTCt                                                                                                   >  2:29110/1‑149 (MQ=255)
                                                 aGGTGCAGCAGGATGCCCAGCCGGCCGGAGGCCGCCGCCCCCGCCCTTTGATGGGTCGCCT‑GAACCCCCCCTTTAGCCGGCACCCCGCGCAAGGGCCGGGTCAGGCGGTAAACATCCCCGGTTACAGCCGCCCCGCGCTAACGTTATg                                                                                                  >  1:3412/1‑148 (MQ=255)
                                                 aGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACAGCCCCTGTTACAGCCGCCCCGCGATAACTTTCTgca                                                                                                >  5:242351/1‑147 (MQ=255)
                                                                 ccAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGGGATAACTTTCTGAACACCTGCTACGTTTAc                                                                                <  1:29110/149‑1 (MQ=255)
                                                                  cAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTAc                                                                                >  1:61326/1‑148 (MQ=255)
                                                                      cggcc‑gAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTc                                                                           <  7:194444/149‑1 (MQ=255)
                                                                      cggcc‑gAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTc                                                                           <  2:61326/149‑1 (MQ=255)
                                                                        gcc‑gAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCAt                                                                         <  6:73066/149‑1 (MQ=255)
                                                                        gcc‑gAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGAAAACTTTCTGAACACCTGCTACGTTTACCACTCa                                                                          >  6:332962/1‑148 (MQ=255)
                                                                               gccgccgccCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCtt                                                                   <  3:118171/149‑1 (MQ=255)
                                                                                ccgccgccCCCTCCCTTTGATTGGTAGCAT‑GAACCTAACATTTCTCCGGCATCCAGCGAAATGGCAGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCTATAACTTTCTTAACACCTGCTACGTTTACCACTCATAAGCtt                                                                   <  7:291049/148‑1 (MQ=255)
                                                                                        ccctccctTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAACGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTGTGAACACCTGCGACGTTTACCACTCATTAGCTTGTGatcatc                                                          >  3:21210/1‑149 (MQ=255)
                                                                                           tccgtTTGATGGGTCGCCT‑GAAGCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCAt                                                                         <  6:109253/131‑1 (MQ=255)
                                                                                           tccctTTGATGGGTCGCCT‑GAACCGACCCTTTAGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAAGACCTGCTACGTTTACCACTCAt                                                                         >  5:109253/1‑131 (MQ=255)
                                                                                             cctTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCtt                                                      >  8:176980/1‑148 (MQ=255)
                                                                                              ctTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACt                                                                            >  3:82510/1‑125 (MQ=255)
                                                                                              ctTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACt                                                                            <  4:82510/125‑1 (MQ=255)
                                                                                                            cT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGtt                                                                                   <  5:188744/104‑1 (MQ=255)
                                                                                                            cT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGtt                                                                                   >  6:188744/1‑104 (MQ=255)
                                                                                                             t‑GAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTa                                       >  1:32515/1‑147 (MQ=38)
                                                                                                             t‑GAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTa                                       <  2:32515/147‑1 (MQ=38)
                                                                                                                 aCCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCt                                   >  6:172114/1‑148 (MQ=255)
                                                                                                                  ccGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTg                                  >  1:257445/1‑148 (MQ=255)
                                                                                                                   cggcccTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAgg                                     <  3:48605/141‑1 (MQ=38)
                                                                                                                   cggcccTTTCGCGGGCTCCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTCTAACAGACGCCCCGCGATAACTTTCTGAACACCAGCAACCTTTACCACTCATAAGCTAGTCATCATCCATTCATCCCACCTGCTTAGGcacat                                >  4:48605/4‑145 (MQ=25)
                                                                                                                        cTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa                           <  3:281532/149‑1 (MQ=255)
                                                                                                                         tttCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa                           >  3:91637/1‑148 (MQ=255)
                                                                                                                           tCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGAt                          <  7:176980/147‑1 (MQ=255)
                                                                                                                                     cccGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAgg               >  6:120848/1‑148 (MQ=255)
                                                                                                                                      ccGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGAt                          <  4:9594/136‑1 (MQ=255)
                                                                                                                                      ccGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGAt                          >  3:9594/1‑136 (MQ=255)
                                                                                                                                         cgAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCaaaa          <  6:178190/149‑1 (MQ=255)
                                                                                                                                           aaaGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAAt         >  6:53442/1‑148 (MQ=255)
                                                                                                                                             aGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAATGAg      <  5:53442/149‑1 (MQ=255)
                                                                                                                                                gCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAATGAGCaa   <  2:257445/149‑1 (MQ=255)
                                                                                                                                                 ccGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAATGAGCaaa  <  5:332962/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCC‑GAGGCCGCCGCCCCCTCCCTTTGATGGGTCGCCT‑GAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAATGAGCAAA  >  NC_002947/2272937‑2273228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑