Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,281,633 G→C 8.2% G152A (GGC→GCC) ‡ PP_2009 → 1‑aminocyclopropane‑1‑carboxylate deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,281,6330GC 8.2% 139.9 / 3.9 49G152A (GGC→GCC) ‡PP_20091‑aminocyclopropane‑1‑carboxylate deaminase
Reads supporting (aligned to +/- strand):  ref base G (25/20);  new base C (2/2);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

CTGGGCATGGAACTGCATTGGCTAGGCTACGGCGGCTACCGTGCACGCAACGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTGGCCGAGGCGGGTGCACATGCCGTGCA  >  NC_002947/2281494‑2281774
                                                                                                                                           |                                                                                                                                               
ctgggcatggAACTGCATTGGCTAGGCTACGGCGGCTACCGTGCACGCAACGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCAGTGCGCAGCCCTGCGCacc                                                                                                                                        <  2:159458‑M2/139‑2 (MQ=255)
    gcatggAACTGCATTGGCTAGGCTACGGCGGCTACCGTGCACGCAACGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATc                                                                                                                                    <  1:34716‑M2/143‑1 (MQ=255)
                       aggctaCGGCGGCTACCGTGCACGCAACGAACCCGGCTTCTGGGAACCTTGTCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGgcgcgcgcg                                                                                                                  >  1:85310/1‑148 (MQ=255)
                         gctaCGGCGGCTACCGTGCACGCAACGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGcagcag                                                                                                                           >  4:123305/1‑137 (MQ=255)
                         gctaCGGCGGCTACCGTGCACGCAACGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGcagcag                                                                                                                           <  3:123305/137‑1 (MQ=255)
                                cggcTACCGTGCACGCAACGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCACAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCgg                                                                                                        <  4:44242/149‑1 (MQ=255)
                                   cTACCGTGCACGCAACGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGAt                                                                                                     >  3:129559/1‑149 (MQ=255)
                                      ccGTGCACGCAACGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTgg                                                                                                  <  2:85310/149‑1 (MQ=255)
                                         tGCACGCAACGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCt                                                                                               >  6:141533/1‑149 (MQ=255)
                                              gCAACGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGt                                                                                            >  7:95920/1‑147 (MQ=255)
                                                aaCGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGa                                                                                        >  5:11047/1‑149 (MQ=255)
                                                aaCGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGa                                                                                        >  6:338113/1‑149 (MQ=255)
                                                    aaCCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCAt                                                                                    <  6:325572/149‑1 (MQ=255)
                                                    aaCCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCACAGATCGCGGGATTGGGCTGGTCAGACCAt                                                                                    >  2:258760/1‑149 (MQ=255)
                                                                 ggAACCTTGGCAAGCGCGCTATCCAGTCTGGCACTCCATCCCCGAAGGGGGCGGCGGGCTGGCCGTTCCGCACGGCTGCGCACAGATCATCCAGCAGGAGCGCGCGCAGATCGCGGGATTTGGCTGGTCAGACCATGACGACTGGtggc                                                                       >  3:203021/1‑149 (MQ=255)
                                                                 ggAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGtggc                                                                       >  2:79013/1‑149 (MQ=255)
                                                                 ggAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGtggc                                                                       >  1:51624/1‑149 (MQ=255)
                                                                 ggAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGtggc                                                                       >  4:97861/1‑149 (MQ=255)
                                                                  gAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGtggct                                                                      <  6:11047/149‑1 (MQ=255)
                                                                     ccTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGtggctg‑gc                                                                  <  3:97861/149‑1 (MQ=255)
                                                                      cTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTggctg‑gct                                                                 >  2:85361/1‑149 (MQ=255)
                                                                       ttGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGgctg‑gctg                                                                >  1:165803/1‑149 (MQ=255)
                                                                          gCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑cc                                                             <  6:295357/148‑1 (MQ=255)
                                                                                gcgcTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACgg                                                      <  5:338113/149‑1 (MQ=255)
                                                                                gcgcTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACgg                                                      <  7:53609/149‑1 (MQ=255)
                                                                                      tCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTa                                                <  1:79013/149‑1 (MQ=255)
                                                                                         aGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCt                                             >  8:53414/1‑149 (MQ=255)
                                                                                          ggCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCtt                                            >  7:168525/1‑149 (MQ=255)
                                                                                           gCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTg                                           >  1:165351/1‑149 (MQ=255)
                                                                                             tGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTggc                                         >  5:123709/1‑149 (MQ=255)
                                                                                               gCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTggc                                         <  7:53414/147‑1 (MQ=255)
                                                                                                     cATCCCCGAAGGGGGCGGCCGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTg                                 >  8:152808/1‑149 (MQ=255)
                                                                                                         cccGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACt                             >  6:260649/1‑149 (MQ=255)
                                                                                                          ccGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTa                               <  6:84834/146‑1 (MQ=255)
                                                                                                          ccGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTa                               >  5:84834/1‑146 (MQ=255)
                                                                                                          ccGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTg                            >  6:129307/1‑149 (MQ=255)
                                                                                                          ccGAAGGGGGCGGCGGGCTGGCCGCTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGGAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTg                            >  6:154506/1‑149 (MQ=255)
                                                                                                           cGAATGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGGAGGCGCGCGCGAAGATCGCGTGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTgg                           <  4:203021/149‑1 (MQ=255)
                                                                                                                    cggcggGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTGGCCGAGGCgg                  <  8:168525/149‑1 (MQ=255)
                                                                                                                     ggcggGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTGGCCGAGGCggg                 >  4:96848/1‑149 (MQ=255)
                                                                                                                       cggGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTGCGCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTGGCCGAGGCGGGt                <  1:85361/149‑1 (MQ=255)
                                                                                                                          gCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTGGCCGAGGCGGGTGca             >  8:78155/1‑148 (MQ=255)
                                                                                                                              gCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCACAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTGCCCTGAACGGGGACTACCTTGGCCGGCCTTGTACTGGCCGAGGCGGGTGCACATg         <  1:258760/149‑1 (MQ=255)
                                                                                                                               tcagTGCGCAGCCCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTg                                           <  1:264796/110‑1 (MQ=255)
                                                                                                                               tcagTGCGCAGCCCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTg                                           >  2:264796/4‑113 (MQ=255)
                                                                                                                                 ggTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTGGCCGAGGCGGGTGCACATGCCGt     <  6:123709/149‑1 (MQ=255)
                                                                                                                                 ggTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTGGCCGAGGCGGGTGCACATGCCGt     <  5:141533/149‑1 (MQ=255)
                                                                                                                                  gTGCGCAGCCCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTGGCCGAGGCGGGTGCACATGCCGTg    >  3:267039/1‑149 (MQ=255)
                                                                                                                                    gcgcAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTGGCCGAGGCGGGTGCACATGCCGTGCa  <  5:260649/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                               
CTGGGCATGGAACTGCATTGGCTAGGCTACGGCGGCTACCGTGCACGCAACGAACCCGGCTTCTGGGAACCTTGGCAAGCGCGCTATCCAGGCTGGCACTGCATCCCCGAAGGGGGCGGCGGGCTGGCCGGTGCGCAGGGCTGCGCACTGATCATGCAGCAGGCGCGCGCGCAGATCGCGGGATTGGGCTGGTCAGACCATGACGCCTGGTGGCTG‑GCTG‑CCGGAACGGGGACTACCTTGGCCGGCCTTGTACTGGCCGAGGCGGGTGCACATGCCGTGCA  >  NC_002947/2281494‑2281774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑