Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 211,621 G→C 8.0% V5709V (GTG→GTC)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947211,6210GC 8.0% 154.1 / 4.8 50V5709V (GTG→GTC) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (26/20);  new base C (2/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

TGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACT  >  NC_002947/211477‑211762
                                                                                                                                                |                                                                                                                                             
tGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGAcc                                                                                                                                            >  2:334910/1‑148 (MQ=255)
tGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGAcc                                                                                                                                            >  4:168954/1‑148 (MQ=255)
          aGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGgtggtg                                                                                                                                  <  5:155961/148‑1 (MQ=255)
          aGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTCACCGAAggcgtcg                                                                                                                                  <  8:123109/148‑6 (MQ=255)
           gCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTgag                                                                                                                                <  1:334910/149‑1 (MQ=255)
            ccTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTgaga                                                                                                                               <  3:11818/149‑1 (MQ=255)
             cTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGAt                                                                                                                              <  5:310323/149‑1 (MQ=255)
                 gCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATAAcc                                                                                                                          >  7:305487/1‑149 (MQ=255)
                   aTCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAggtggt                                                                                                                                   <  6:201059/138‑1 (MQ=255)
                   aTCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAggtggt                                                                                                                                   >  5:201059/1‑138 (MQ=255)
                     cAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCAcc                                                                                                                          <  4:30297/145‑1 (MQ=255)
                     cAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCAcc                                                                                                                          >  3:30297/1‑145 (MQ=255)
                      aaCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGaa                                                                                                                                         >  6:118523/1‑129 (MQ=255)
                      aaCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGaa                                                                                                                                         <  5:118523/129‑1 (MQ=255)
                         tCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCAt                                                                                                                  >  5:50443/1‑149 (MQ=255)
                                            gTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGAt                                                                                               <  6:50443/149‑1 (MQ=255)
                                                gACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAggtgg                                                                                                                                    <  3:201468/108‑1 (MQ=255)
                                                gACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAggtgg                                                                                                                                    >  4:201468/1‑108 (MQ=255)
                                                      cGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGcc                                                                                       <  4:252805/147‑1 (MQ=255)
                                                       gagaACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGc                                                                                        >  8:210608/1‑145 (MQ=255)
                                                       gagaACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGc                                                                                        <  7:210608/145‑1 (MQ=255)
                                                        agaACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGAt                                                                                    >  2:179152/1‑148 (MQ=255)
                                                                     gggCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGa                                                                                                                                              >  3:86721/1‑77 (MQ=39)
                                                                     gggCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGa                                                                                                                                              <  4:86721/77‑1 (MQ=39)
                                                                                cTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACAGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACAAACAAAGATGGTCTGCCGATCAACAACCACAGTGCAATGActat                                                            >  6:202824/1‑145 (MQ=255)
                                                                                       tCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCt                                                      >  1:92642/1‑147 (MQ=255)
                                                                                        cAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAggtggt                                                                                                                                   <  6:350035/69‑1 (MQ=255)
                                                                                        cAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAggtggt                                                                                                                                   >  5:350035/1‑69 (MQ=255)
                                                                                        cAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGa                                                    <  1:179152/148‑1 (MQ=255)
                                                                                            tGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGAc                                               >  6:224417/1‑149 (MQ=255)
                                                                                                cGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGc                                            >  1:323935/1‑148 (MQ=255)
                                                                                                      caccGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCAc                                                                                                                           >  3:288257/1‑63 (MQ=255)
                                                                                                      caccGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCAc                                                                                                                           <  4:288257/63‑1 (MQ=255)
                                                                                                      caccGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATg                                                                                              <  7:286881/92‑1 (MQ=255)
                                                                                                      caccGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATg                                                                                              >  8:286881/1‑92 (MQ=255)
                                                                                                      caccGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCg                                     >  2:108307/1‑149 (MQ=255)
                                                                                                      caccGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCg                                     >  1:228844/1‑149 (MQ=255)
                                                                                                      caccGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCg                                     <  2:323935/149‑1 (MQ=255)
                                                                                                             gaAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCATGAGCGACGGCAAAACCGTCATCAc                              >  8:51375/1‑149 (MQ=255)
                                                                                                                      gCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCgg                      >  4:5371/1‑148 (MQ=255)
                                                                                                                      gCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCGGAGCGACGGCAAAACCGTCATCACCGTGCCGGc                     >  4:37606/1‑149 (MQ=255)
                                                                                                                         aaGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTaa                  >  3:3520/1‑149 (MQ=255)
                                                                                                                          aGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAAt                 <  1:108307/149‑1 (MQ=255)
                                                                                                                          aGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAAt                 >  3:72337/1‑149 (MQ=255)
                                                                                                                          aGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATCGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAAt                 <  8:305487/149‑1 (MQ=255)
                                                                                                                               aCCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCa             <  5:224417/148‑1 (MQ=255)
                                                                                                                                      caccTTCGGTCACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTggg      >  7:15803/3‑148 (MQ=255)
                                                                                                                                      caccTTCGGTCACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTACATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGc                         <  4:179114/127‑1 (MQ=255)
                                                                                                                                      caccTTCGGTCACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTACATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGc                         >  3:179114/3‑129 (MQ=255)
                                                                                                                                         cTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACt  >  1:170308/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                             
TGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACT  >  NC_002947/211477‑211762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑