Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,303,525	C→T	6.1%	T439T (ACG→ACA)	PP_2027 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,303,525	0	C	T	6.1%	195.3 / 2.3	67	T439T (ACG→ACA)	PP_2027	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/37);  new base T (2/2);  total (27/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

CACGAAGCGCGCCGCCATCTCGGCCAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGGCGCTGCTGTTGTCGCCCACCGGGGTGTCGGCG  >  NC_002947/2303378‑2303669                                                                                                                                                    |                                                                                                                                                  cACGAAGCGCGCCGCCATCTCGGCCAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCg                                                                                                                                                  <  1:154860/149‑1 (MQ=255)    gAAGCGCGCCGCCATCTCGGCCAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTgc                                                                                                                                               <  7:126814/149‑1 (MQ=255)      aGCGCGCCGCCATCTCGGCCAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTgcgc                                                                                                                                             <  5:308655/149‑1 (MQ=255)           gccgccATCTCGGCCAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCAc                                                                                                                                        <  2:276503/149‑1 (MQ=255)           gccgccATCTCGGCCAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCACGGCGCAGAAc                                                                                                                                        <  2:231930/149‑1 (MQ=255)            ccgccATCTCGGCCAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCAcc                                                                                                                                       <  6:327759/149‑1 (MQ=255)               ccATCTCGGCCAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCt                                                                                                                                    <  8:47075/149‑1 (MQ=255)                      ggCCAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCcgc                                                                                                                              >  4:205367/1‑148 (MQ=255)                         cAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAAc                                                                                                                          >  1:104532/1‑149 (MQ=255)                         cAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAAc                                                                                                                          >  6:114566/1‑149 (MQ=255)                         cAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAAc                                                                                                                          <  1:84274/149‑1 (MQ=255)                              tttCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTCGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGgcg                                                                                                                     >  7:27767/1‑149 (MQ=255)                               ttCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGGGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGgcgc                                                                                                                    <  8:271156/149‑1 (MQ=255)                                tCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGgcgc                                                                                                                    <  6:25004/148‑1 (MQ=255)                                  cGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCTGTGCGCAGCACCGCTGGCCGCGAACGGgcgcgc                                                                                                                  >  7:131238/1‑148 (MQ=255)                                  cGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCTGTGCGCAGCACCGCTGGCCGCGAACGGgcgcgc                                                                                                                  >  5:48629/1‑148 (MQ=255)                                  cGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGcc                                                                                                                 <  1:159361/149‑1 (MQ=255)                                    gTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGccac                                                                                                               <  2:81849/149‑1 (MQ=255)                                    gTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGccac                                                                                                               >  5:219413/1‑149 (MQ=255)                                    gTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGccac                                                                                                               <  5:114566/149‑1 (MQ=255)                                         tgcCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAg                                                                                                          <  8:27767/149‑1 (MQ=255)                                           ccGGGCCCAGGTATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAg                                                                                                          <  6:213773/147‑1 (MQ=255)                                           ccGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAggg                                                                                                        >  3:17102/1‑149 (MQ=255)                                                cccAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAg                                                                                                   <  6:219413/149‑1 (MQ=255)                                                   aGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACcaca                                                                                                           >  2:119471/1‑138 (MQ=255)                                                       aTCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCa                                                                                            >  5:277310/1‑149 (MQ=255)                                                       aTCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGACTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCa                                                                                            >  2:76560/1‑149 (MQ=255)                                                           aGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACgg‑t                                                                                       >  3:213163/1‑149 (MQ=255)                                                           aGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACgg‑t                                                                                       <  1:190394/149‑1 (MQ=255)                                                            gcgcCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACgg‑tg                                                                                      <  7:162428/149‑1 (MQ=255)                                                                   gcgcGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTTTTTTTGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAgg                                                                                <  1:287285/148‑1 (MQ=255)                                                                   gcgcGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAgg                                                                                <  3:171744/148‑1 (MQ=255)                                                                    cgcgTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGc                                                                              <  4:83870/149‑1 (MQ=255)                                                                         aGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACggg                                                                                                                       <  3:318369/104‑1 (MQ=255)                                                                         aGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACggg                                                                                                                       >  4:318369/1‑104 (MQ=255)                                                                               ggggTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAGGCCCAGCACGG‑TGGTCAGGGCAAACACCAgt                                                                    <  4:17102/148‑1 (MQ=255)                                                                                   tCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGa                                                               <  5:143525/149‑1 (MQ=255)                                                                                    cGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGtgtg                                                                 >  5:130269/1‑146 (MQ=255)                                                                                    cGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGtgtg                                                                 <  6:130269/146‑1 (MQ=255)                                                                                     gTTGGACTGGCATGCGCGCTTGAGGTCGTCCAGCTAGGGGCGTTGGCTTGGCCCGTTCTGCGGCGTGCGCAGCACCACTGGCCGCGAACGGGCGCGGCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGAc                                                              <  8:232819/148‑1 (MQ=255)                                                                                          ccTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACgg‑tg                                                                                      <  1:264230/119‑1 (MQ=255)                                                                                          ccTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACgg‑tg                                                                                      >  2:264230/1‑119 (MQ=255)                                                                                          ccTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGc                                                        >  7:323159/1‑149 (MQ=255)                                                                                          ccTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGc                                                        >  1:302601/1‑149 (MQ=255)                                                                                            tGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGa                                                               <  7:287210/140‑1 (MQ=255)                                                                                            tGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGa                                                               >  8:287210/1‑140 (MQ=255)                                                                                                    gcgcTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACgg                                                                                         >  6:269250/1‑107 (MQ=255)                                                                                                    gcgcTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACgg                                                                                         <  5:269250/107‑1 (MQ=255)                                                                                                       cTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCAcagca                                           <  2:104532/149‑1 (MQ=255)                                                                                                          gAGGTCGTGCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAggg                                        >  1:328240/1‑149 (MQ=255)                                                                                                           aGGTCGTCCAGCAAGGTGCGTGGACTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGGCTGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAgg                                         <  1:76560/148‑1 (MQ=255)                                                                                                             gtcgtcCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGc                                     >  2:206008/1‑149 (MQ=255)                                                                                                                 tcCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCca                                                >  1:341301/1‑134 (MQ=255)                                                                                                                 tcCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCca                                                <  2:341301/134‑1 (MQ=255)                                                                                                                       aaGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGGCgctgct                           <  3:205367/149‑1 (MQ=255)                                                                                                                                 ggCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGGCGCTGCTGTTGTCGcc                  <  4:213163/148‑1 (MQ=255)                                                                                                                                  gCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGGCGCTGCTGTTGTCGCCCa                >  8:323099/1‑149 (MQ=255)                                                                                                                                   cTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGgcg                                <  7:109112/132‑1 (MQ=255)                                                                                                                                   cTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGgcg                                >  8:109112/1‑132 (MQ=255)                                                                                                                                       gCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGGCGCTGCTGTTGTCGCCCACCgg            <  2:328240/148‑1 (MQ=255)                                                                                                                                        cccGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGGCGCTGCTGTTGTCGCCCACCggg           <  5:300729/148‑1 (MQ=255)                                                                                                                                        cccGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGGCGCTGCTGTTGTCGCCCACCggg           >  6:300729/1‑148 (MQ=255)                                                                                                                                         ccGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTG‑CACGGCCCCACGAGGGTGCGTGCGGAGGAGTTGTCGCCCCCCGGGgtg        >  6:55505/1‑149 (MQ=255)                                                                                                                                           gTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGGCGCTGCTGTTGTCGCCCACCGGGgtgt       <  7:300516/149‑1 (MQ=255)                                                                                                                                               tGCGCTGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACATGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGGCGCTGCTGTTGTCGCCCACCGGGGTGTCGgc   <  8:131238/149‑1 (MQ=255)                                                                                                                                                gcgcCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGGCGCTGCTGTTGTTGCCCACCGGGGTGTCGgcg  >  2:81847/1‑149 (MQ=255)                                                                                                                                                 cgcTGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGGCGCTGCTGTTGTCGCCCACCGGGGTGTCGgc   <  6:48629/147‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CACGAAGCGCGCCGCCATCTCGGCCAGGGTTTCCGGTTGCTGCCGGGCCCAGGCATCCAGCGCCTGGCGCGTAGCCTGGGGGTCGTTGGCCTGGCATGCGCGCTTGAGGTCGTCCAGCAAGGTGCGTGGGCTTGGCCCGTTCTGCGCCGTGCGCAGCACCGCTGGCCGCGAACGGGCGCGCCACCACAGGGCAAAGCCCAGCACGG‑TGGTCAGGGCAAACACCAGTGTGGACAGCTGCCAGGGCCACAGCAGGGTGCTGGCGCTGCTGTTGTCGCCCACCGGGGTGTCGGCG  >  NC_002947/2303378‑2303669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑