Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,305,212 G→A 6.6% L449L (CTG→TTG)  PP_2028 ← TPR repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,305,2120GA 6.6% 179.0 / 2.0 60L449L (CTG→TTG) PP_2028TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (30/26);  new base A (2/2);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

CGGGGCTGGCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGTCG  >  NC_002947/2305065‑2305357
                                                                                                                                                   |                                                                                                                                                  
cGGGGCTGGCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGt                                                                                                                                                   >  3:10664/1‑149 (MQ=255)
      tGGCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTtgctgc                                                                                                                                             >  1:295842/1‑149 (MQ=255)
        gCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAc                                                                                                                                           >  8:194086/1‑149 (MQ=255)
        gCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAc                                                                                                                                           <  1:190013/149‑1 (MQ=255)
          cTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAc                                                                                                                                           >  2:21252/1‑147 (MQ=255)
          cTGTTGGTCGGCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCa                                                                                                                                         <  6:16711/149‑1 (MQ=255)
           tGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTtgctg                                                                                                                                              >  4:73388/1‑143 (MQ=255)
           tGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTtgctg                                                                                                                                              <  3:73388/143‑1 (MQ=255)
             ttGGGCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAACTGCTGCAACaac                                                                                                                                       <  5:229531/148‑3 (MQ=255)
                gtcgtcGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCt                                                                                                                                   >  4:73289/1‑149 (MQ=255)
                gtcgtcGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCt                                                                                                                                   >  2:329999/1‑149 (MQ=255)
                gtcgtcACCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGTGCCt                                                                                                                                   >  5:15890/1‑149 (MQ=255)
                  cgtcgCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTgg                                                                                                                                 <  2:92002/149‑1 (MQ=255)
                   gtcgCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTgg                                                                                                                                 <  7:194086/148‑1 (MQ=255)
                    tcgCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGtt                                                                                                                               <  3:73289/149‑1 (MQ=255)
                      gCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGAGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGt                                                                                                                             >  7:131250/1‑149 (MQ=255)
                                               ctgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTg                                                                                                    <  2:125638/149‑1 (MQ=255)
                                                  gCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGa                                                                                                   <  1:21252/147‑1 (MQ=255)
                                                  gCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCCCGACCTGCAGGCCGCGCTgga                                                                                                  <  5:5640/148‑3 (MQ=255)
                                                    cGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCt                                                                                               >  6:334491/1‑149 (MQ=255)
                                                      aGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTc                                                                                              >  4:289047/1‑148 (MQ=255)
                                                        ctgctgTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGtt                                                                                                                               <  2:36466/113‑1 (MQ=255)
                                                        ctgctgTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGtt                                                                                                                               >  1:36466/1‑113 (MQ=255)
                                                           ctgTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGc                                                                                        >  4:18679/1‑149 (MQ=255)
                                                             gTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCtt                                                                                      >  8:303664/1‑149 (MQ=255)
                                                               tGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGAGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGc                                                                                    <  8:131250/149‑1 (MQ=255)
                                                                gCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGc                                                                                    <  4:10664/148‑1 (MQ=255)
                                                                     cGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGTGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTa                                                                               <  6:15890/148‑1 (MQ=255)
                                                                      ggTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAg                                                                              <  4:162653/148‑1 (MQ=255)
                                                                       gTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAgg                                                                             >  1:337573/1‑148 (MQ=255)
                                                                       gTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGGTGCAGCAACTGCTGCAACAacgt                                                                                                                                     <  6:68460/92‑2 (MQ=38)
                                                                       gTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGGTGCAGCAACTGCTGCAACAacgt                                                                                                                                     >  5:68460/1‑91 (MQ=38)
                                                                        tttCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCa                                                                           >  1:300508/1‑149 (MQ=255)
                                                                        tttCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCa                                                                           >  4:113101/1‑149 (MQ=255)
                                                                                ccGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGgg‑c                                                                  <  1:329999/149‑1 (MQ=255)
                                                                                ccGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTAGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGgg‑c                                                                  >  4:217524/1‑149 (MQ=255)
                                                                                   ggCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGgg‑cggc                                                               <  7:303664/149‑1 (MQ=255)
                                                                                       tgtCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTcc                                                           <  2:300508/149‑1 (MQ=255)
                                                                                       tgtCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTcc                                                           <  3:18679/149‑1 (MQ=255)
                                                                                           ttGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCt                                                       >  5:315231/1‑149 (MQ=255)
                                                                                             gggCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCg                                                     >  5:184598/1‑149 (MQ=255)
                                                                                                cATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCa                                                  >  4:123502/1‑149 (MQ=255)
                                                                                                 aTCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCAc                                                 >  5:180254/1‑149 (MQ=255)
                                                                                                          gggCGGTTGTTCCTCGGCCTTGGCGTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCa                                        <  5:208556/149‑1 (MQ=255)
                                                                                                           ggCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGc                                          >  6:208556/1‑146 (MQ=255)
                                                                                                                    tCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGcc                              >  5:26389/1‑149 (MQ=255)
                                                                                                                     ccTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCt                             >  7:253999/1‑149 (MQ=255)
                                                                                                                     ccTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCt                             >  7:249436/1‑149 (MQ=255)
                                                                                                                           ccTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTg                       >  8:72270/1‑149 (MQ=255)
                                                                                                                           ccTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTg                       <  3:153987/149‑1 (MQ=255)
                                                                                                                            cTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTg                       >  7:88764/1‑148 (MQ=255)
                                                                                                                               ggCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGGCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTa                     <  4:250439/147‑1 (MQ=255)
                                                                                                                                gCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGt                   <  6:180254/148‑1 (MQ=255)
                                                                                                                                  ttCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTggg                >  3:177788/1‑149 (MQ=255)
                                                                                                                                    cACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGgc               <  3:123502/148‑1 (MQ=255)
                                                                                                                                     aCGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGgctg             <  6:26389/149‑1 (MQ=255)
                                                                                                                                         tggtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTCCGGGCCAGGGCATTGCGACGAATGTAGTGGcatgctc          >  7:283836/4‑147 (MQ=255)
                                                                                                                                           ttgCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCa        <  4:177788/148‑1 (MQ=255)
                                                                                                                                             gcagcaGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTTGTAGGCATCCAGGGCCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGAtt                        <  1:66074/131‑1 (MQ=255)
                                                                                                                                             gcagcaGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTTGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGAtt                        >  2:66074/1‑130 (MQ=255)
                                                                                                                                               agcagTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCAAGGGCATTGCCTCGATTGTTGTGGGCTGCTGCAgtg     >  8:271537/1‑147 (MQ=255)
                                                                                                                                               agcagTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGATCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGTc   >  6:15526/1‑149 (MQ=255)
                                                                                                                                                gcagTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGTCg  <  3:217524/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGGGCTGGCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGG‑CGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGTCG  >  NC_002947/2305065‑2305357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑