Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,340,703 A→C 7.5% intergenic (+37/‑78) dctA‑II → / → yraO C4‑dicarboxylate transport protein/transporter YraO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,340,7030AC 7.5% 151.7 / 3.6 53intergenic (+37/‑78)dctA‑II/yraOC4‑dicarboxylate transport protein/transporter YraO
Reads supporting (aligned to +/- strand):  ref base A (23/26);  new base C (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

AAACGCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCAC  >  NC_002947/2340558‑2340850
                                                                                                                                                 |                                                                                                                                                    
aaaCGCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGc                                                                                                                                                   >  6:141063/1‑149 (MQ=255)
    gCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTg                                                                                                                                               >  3:9012/1‑149 (MQ=255)
              gCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCTCACCCGGCAACGCCTGGTgggg                                                                                                                                     <  4:80711/149‑1 (MQ=255)
                          acaGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGCGCCACGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCg                                                                                                                         >  6:246866/1‑149 (MQ=255)
                            aGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTc                                                                                                                       >  2:63912/1‑149 (MQ=255)
                             gCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCt                                                                                                                      >  8:193889/1‑149 (MQ=255)
                              ccGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTg                                                                                                                     <  7:71236/149‑1 (MQ=255)
                                          cGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTg                                                                                                          <  5:133397/148‑1 (MQ=255)
                                          cGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTg                                                                                                          >  6:133397/1‑148 (MQ=255)
                                                ggtggtCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACAt                                                                                                   <  3:160696/149‑1 (MQ=255)
                                                 gtggtCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATa                                                                                                  <  4:37645/149‑1 (MQ=255)
                                                    gtCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGAc                                                                                               <  1:63912/149‑1 (MQ=255)
                                                       aCCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCa                                                                                                              >  1:1815/1‑131 (MQ=255)
                                                       aCCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCa                                                                                                              <  2:1815/131‑1 (MQ=255)
                                                         ccccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGAc                                                                                               >  7:225787/1‑144 (MQ=255)
                                                         ccccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGAc                                                                                               <  8:225787/144‑1 (MQ=255)
                                                                 cTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAaga                                                                                  <  6:330027/149‑1 (MQ=255)
                                                                 cTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAaga                                                                                  <  6:68030/149‑1 (MQ=255)
                                                                    aaaCAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGaa                                                                               <  5:141063/149‑1 (MQ=255)
                                                                     aaCAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGaa                                                                               >  4:306641/1‑148 (MQ=255)
                                                                       cAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACa                                                                             >  4:93573/1‑148 (MQ=255)
                                                                            gACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGTGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCAt                                                                       <  7:21146/149‑1 (MQ=255)
                                                                                        gAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCt                                                           >  6:38140/1‑149 (MQ=255)
                                                                                                  gCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATc                                                 <  3:93573/149‑1 (MQ=255)
                                                                                                       ggCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACAt                                                                                                   <  6:157678/94‑1 (MQ=255)
                                                                                                       ggCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACAt                                                                                                   >  5:157678/1‑94 (MQ=255)
                                                                                                              ccccGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGc                                                       <  6:181797/131‑1 (MQ=255)
                                                                                                              ccccGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGc                                                       >  5:181797/1‑131 (MQ=255)
                                                                                                                       ccTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCcaca                                                                                                    <  1:261534/77‑1 (MQ=255)
                                                                                                                       ccTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCt                                                           <  5:244977/118‑1 (MQ=255)
                                                                                                                       ccTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCt                                                           >  6:244977/1‑118 (MQ=255)
                                                                                                                       ccTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCacctac                           <  7:193889/149‑1 (MQ=255)
                                                                                                                        cTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAt‑ggtgg                                         >  4:58500/1‑134 (MQ=255)
                                                                                                                        cTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAt‑ggtgg                                         <  3:58500/134‑1 (MQ=255)
                                                                                                                        cTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCacctacc                          >  2:71556/1‑149 (MQ=255)
                                                                                                                           aaGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGa                       >  8:224790/1‑149 (MQ=255)
                                                                                                                             gCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATc                     <  5:38140/149‑1 (MQ=255)
                                                                                                                             gCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTATGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATc                     <  5:47212/149‑1 (MQ=255)
                                                                                                                               tttttGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCAt                   >  3:280037/1‑149 (MQ=255)
                                                                                                                               tttttGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCAt                   <  7:224790/149‑1 (MQ=255)
                                                                                                                                 tttGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATg                  <  3:306641/148‑1 (MQ=255)
                                                                                                                                  ttGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAG‑GTAGGTGAAGGTCACCACCATGATCaggc                 <  4:9012/148‑4 (MQ=38)
                                                                                                                                    gCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGt               >  3:122581/1‑148 (MQ=255)
                                                                                                                                      tgacccccACCCGGCAACGCCTGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCacctacct                         >  7:249911/4‑136 (MQ=255)
                                                                                                                                      tgacccccACCCGGCAACGCCTGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCacctacct                         <  8:249911/133‑1 (MQ=255)
                                                                                                                                        gCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACg          >  6:314829/1‑149 (MQ=255)
                                                                                                                                         cccccACCCGGCAACGCCTGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGc         >  2:286571/1‑149 (MQ=255)
                                                                                                                                          ccccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCa                                >  5:1949/1‑125 (MQ=255)
                                                                                                                                          ccccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCa                                <  6:1949/125‑1 (MQ=255)
                                                                                                                                          ccccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCAt                   <  3:157391/138‑1 (MQ=255)
                                                                                                                                          ccccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCAt                   >  4:157391/1‑138 (MQ=255)
                                                                                                                                          ccccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGcc        >  2:114314/1‑149 (MQ=255)
                                                                                                                                           cccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACTAACCATGCTCTCAATTCTGGGCCTTATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCt       <  5:246866/149‑1 (MQ=255)
                                                                                                                                                caGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACATTAAAAACAAGAGAACTAACCTTGCTCGCACTTCTGGGCGTGTTCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCGTGTCAc  <  1:6028/149‑1 (MQ=255)
                                                                                                                                                 aGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATCGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCAc  <  1:71556/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AAACGCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCAC  >  NC_002947/2340558‑2340850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑