Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,340,707 G→A 7.2% intergenic (+41/‑74) dctA‑II → / → yraO C4‑dicarboxylate transport protein/transporter YraO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,340,7070GA 7.2% 159.4 / 2.4 55intergenic (+41/‑74)dctA‑II/yraOC4‑dicarboxylate transport protein/transporter YraO
Reads supporting (aligned to +/- strand):  ref base G (23/28);  new base A (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCACCGATC  >  NC_002947/2340562‑2340855
                                                                                                                                                 |                                                                                                                                                     
gCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTg                                                                                                                                                    >  3:9012/1‑149 (MQ=255)
          gCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCTCACCCGGCAACGCCTGGTgggg                                                                                                                                          <  4:80711/149‑1 (MQ=255)
                      acaGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGCGCCACGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCg                                                                                                                              >  6:246866/1‑149 (MQ=255)
                        aGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTc                                                                                                                            >  2:63912/1‑149 (MQ=255)
                         gCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCt                                                                                                                           >  8:193889/1‑149 (MQ=255)
                          ccGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTg                                                                                                                          <  7:71236/149‑1 (MQ=255)
                                      cGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTg                                                                                                               <  5:133397/148‑1 (MQ=255)
                                      cGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTg                                                                                                               >  6:133397/1‑148 (MQ=255)
                                            ggtggtCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACAt                                                                                                        <  3:160696/149‑1 (MQ=255)
                                             gtggtCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATa                                                                                                       <  4:37645/149‑1 (MQ=255)
                                                gtCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGAc                                                                                                    <  1:63912/149‑1 (MQ=255)
                                                   aCCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCa                                                                                                                   >  1:1815/1‑131 (MQ=255)
                                                   aCCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCa                                                                                                                   <  2:1815/131‑1 (MQ=255)
                                                     ccccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGAc                                                                                                    >  7:225787/1‑144 (MQ=255)
                                                     ccccGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGAc                                                                                                    <  8:225787/144‑1 (MQ=255)
                                                             cTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAaga                                                                                       <  6:330027/149‑1 (MQ=255)
                                                             cTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAaga                                                                                       <  6:68030/149‑1 (MQ=255)
                                                                aaaCAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGaa                                                                                    <  5:141063/149‑1 (MQ=255)
                                                                 aaCAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGaa                                                                                    >  4:306641/1‑148 (MQ=255)
                                                                   cAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACa                                                                                  >  4:93573/1‑148 (MQ=255)
                                                                        gACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGTGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCAt                                                                            <  7:21146/149‑1 (MQ=255)
                                                                                    gAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCt                                                                >  6:38140/1‑149 (MQ=255)
                                                                                              gCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATc                                                      <  3:93573/149‑1 (MQ=255)
                                                                                                   ggCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACAt                                                                                                        <  6:157678/94‑1 (MQ=255)
                                                                                                   ggCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACAt                                                                                                        >  5:157678/1‑94 (MQ=255)
                                                                                                          ccccGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGc                                                            <  6:181797/131‑1 (MQ=255)
                                                                                                          ccccGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGc                                                            >  5:181797/1‑131 (MQ=255)
                                                                                                                   ccTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCcaca                                                                                                         <  1:261534/77‑1 (MQ=255)
                                                                                                                   ccTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCt                                                                <  5:244977/118‑1 (MQ=255)
                                                                                                                   ccTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCt                                                                >  6:244977/1‑118 (MQ=255)
                                                                                                                   ccTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCacctac                                <  7:193889/149‑1 (MQ=255)
                                                                                                                    cTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAt‑ggtgg                                              >  4:58500/1‑134 (MQ=255)
                                                                                                                    cTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAt‑ggtgg                                              <  3:58500/134‑1 (MQ=255)
                                                                                                                    cTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCacctacc                               >  2:71556/1‑149 (MQ=255)
                                                                                                                       aaGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGa                            >  8:224790/1‑149 (MQ=255)
                                                                                                                         gCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATc                          <  5:38140/149‑1 (MQ=255)
                                                                                                                         gCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTATGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATc                          <  5:47212/149‑1 (MQ=255)
                                                                                                                           tttttGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCAt                        <  7:224790/149‑1 (MQ=255)
                                                                                                                           tttttGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCAt                        >  3:280037/1‑149 (MQ=255)
                                                                                                                             tttGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATg                       <  3:306641/148‑1 (MQ=255)
                                                                                                                              ttGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAG‑GTAGGTGAAGGTCACCACCATGATCaggc                      <  4:9012/148‑4 (MQ=38)
                                                                                                                                gCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGt                    >  3:122581/1‑148 (MQ=255)
                                                                                                                                  tgacccccACCCGGCAACGCCTGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCacctacct                              >  7:249911/4‑136 (MQ=255)
                                                                                                                                  tgacccccACCCGGCAACGCCTGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCacctacct                              <  8:249911/133‑1 (MQ=255)
                                                                                                                                    gCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACg               >  6:314829/1‑149 (MQ=255)
                                                                                                                                     cccccACCCGGCAACGCCTGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGc              >  2:286571/1‑149 (MQ=255)
                                                                                                                                      ccccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCa                                     >  5:1949/1‑125 (MQ=255)
                                                                                                                                      ccccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCa                                     <  6:1949/125‑1 (MQ=255)
                                                                                                                                      ccccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCAt                        <  3:157391/138‑1 (MQ=255)
                                                                                                                                      ccccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCAt                        >  4:157391/1‑138 (MQ=255)
                                                                                                                                      ccccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGcc             >  2:114314/1‑149 (MQ=255)
                                                                                                                                       cccACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACTAACCATGCTCTCAATTCTGGGCCTTATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCt            <  5:246866/149‑1 (MQ=255)
                                                                                                                                            caGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACATTAAAAACAAGAGAACTAACCTTGCTCGCACTTCTGGGCGTGTTCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCGTGTCAc       <  1:6028/149‑1 (MQ=255)
                                                                                                                                             aGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCATCGGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCAc       <  1:71556/149‑1 (MQ=255)
                                                                                                                                              ggCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCAcc      >  7:251887/1‑148 (MQ=255)
                                                                                                                                                 gTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCACCGAtc  >  6:66576/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GCCTGTGACCGCCAGACGCTGCACAGCCGGCTGAACGGCGAGCCGGTGGTCACCCCCGTCGCTGAAACAGCCGACTGGGTCGCCGAACCACACCGCCACGGCTGAGCCCCGACACCCTCAAGCTTTTTGCAGGCCCCCACCAGGCGTTGCCGGGTGGGGGTCAGGGGGCCGTCTGCGCTGCAGGTGGCCACATAGACAATAAAAACAAGAGAACAAACCATGCTCGCACTTCTGGGCCTGATCAT‑GGTGGTGACCTTCACCTACCTGATCATGAGTAAACGCCTGTCACCGATC  >  NC_002947/2340562‑2340855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑