Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 226,663 G→A 8.0% intergenic (+70/‑63) PP_0174 → / → PP_0175 hypothetical protein/transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947226,6630GA 8.0% 142.4 / 2.2 50intergenic (+70/‑63)PP_0174/PP_0175hypothetical protein/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (22/24);  new base A (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GCGGGACCGGGCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGGCGCATCTGTCATACCGCCTT  >  NC_002947/226521‑226811
                                                                                                                                              |                                                                                                                                                    
gCGGGACCGGGCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAggg                                                                                                                                                >  4:283988/1‑149 (MQ=255)
 cGGGACCGGGCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAgggg                                                                                                                                               >  8:187741/1‑149 (MQ=255)
   ggACCGGGCGGTGCAGCTGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTCGTCAACAgggggc                                                                                                                                             <  8:307142/149‑2 (MQ=255)
    gACCGGGCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGAt                                                                                                                                            <  3:3608/149‑1 (MQ=255)
    gACCGGGCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGAt                                                                                                                                            >  2:157280/1‑149 (MQ=255)
       cGGGCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTc                                                                                                                                         <  8:245462/149‑1 (MQ=255)
          gCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCggg                                                                                                                                      <  7:273004/149‑1 (MQ=255)
          gCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCggg                                                                                                                                      <  4:94769/149‑1 (MQ=255)
              tGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGCCg                                                                                                                                   >  3:56329/1‑148 (MQ=255)
                  gATGTGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTatat                                                                                                                              <  2:242077/149‑1 (MQ=255)
                  gATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGGGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTatat                                                                                                                              >  2:308652/1‑149 (MQ=255)
                   aTGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATAtt                                                                                                                             >  3:13287/1‑149 (MQ=255)
                       gcagcaCATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATa                                                                                                                         <  3:283988/149‑1 (MQ=255)
                         agcaCATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGt                                                                                                                                  <  2:52466/138‑1 (MQ=255)
                         agcaCATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGt                                                                                                                                  >  1:52466/1‑138 (MQ=255)
                           cacaTTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTa                                                                                                                     <  2:202548/149‑1 (MQ=255)
                                             gAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACt                                                                                                   >  4:160870/1‑149 (MQ=255)
                                             gAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTCGTCAACAggggg                                                                                                                                              <  8:144850/106‑1 (MQ=255)
                                             gAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTCGTCAACAggggg                                                                                                                                              >  7:144850/1‑106 (MQ=255)
                                                gCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTgg                                                                                                <  1:157280/149‑1 (MQ=255)
                                                       gACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCtt                                                                                         <  8:104919/149‑1 (MQ=255)
                                                          cAGATCCATCCCTGGGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCAt                                                                                      <  1:308652/149‑1 (MQ=255)
                                                            gATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGt                                                                                    <  7:187741/149‑1 (MQ=255)
                                                            gATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGt                                                                                    >  5:52602/1‑149 (MQ=255)
                                                              tccatccCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTcc                                                                                  <  3:160870/149‑1 (MQ=255)
                                                                catccCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCt                                                                                >  5:241927/1‑149 (MQ=255)
                                                                 atccCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCtt                                                                               <  4:249687/149‑1 (MQ=255)
                                                                  tccCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGTATATACTTGGTTCTCTTCATGTCCCtt                                                                               >  7:168964/1‑148 (MQ=255)
                                                                    ccTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGa                                                                             <  6:241927/148‑1 (MQ=255)
                                                                       gAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGAttt                                                                          >  1:276585/1‑148 (MQ=255)
                                                                        aGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTc                                                                         <  5:104998/148‑1 (MQ=255)
                                                                              tctcTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGa                                                                                                          >  8:302959/1‑109 (MQ=255)
                                                                              tctcTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGa                                                                                                          <  7:302959/109‑1 (MQ=255)
                                                                                          tgactgaAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTcag                                                        >  6:325289/1‑147 (MQ=255)
                                                                                              tgaAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTgg                                                  >  8:137039/1‑149 (MQ=255)
                                                                                                 aaaaTGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGcc                                                <  6:52602/148‑1 (MQ=255)
                                                                                                  aaaTGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGATTATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTg                                              <  4:56329/149‑1 (MQ=255)
                                                                                                   aaTGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTgg                                             <  7:137039/149‑1 (MQ=255)
                                                                                                     tGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTa                                           <  4:13287/149‑1 (MQ=255)
                                                                                                        acaacaTTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTc                                        >  1:137437/1‑149 (MQ=255)
                                                                                                         caacaTTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCg                                       >  8:89979/1‑149 (MQ=255)
                                                                                                           acaTTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCt                                     <  3:96194/149‑1 (MQ=255)
                                                                                                             aTTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTgg                                             >  8:111437/1‑139 (MQ=255)
                                                                                                             aTTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTgg                                             <  7:111437/139‑1 (MQ=255)
                                                                                                                          aTCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTcagca                                                      >  4:98498/1‑117 (MQ=255)
                                                                                                                          aTCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTcagca                                                      <  3:98498/117‑1 (MQ=255)
                                                                                                                             cGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGgcggcg                    >  6:14968/1‑148 (MQ=255)
                                                                                                                                 tcccccTGTCGTCAACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGGCGCATCt               >  5:7682/2‑149 (MQ=255)
                                                                                                                                     ccTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGGCGCATCTGTCa           >  5:347137/1‑149 (MQ=255)
                                                                                                                                         ttGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGGCGCATCTGTCATAcc       >  7:121154/1‑149 (MQ=255)
                                                                                                                                              gacAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGGCGCATCTGTCATACCGCCtt  >  2:98679/1‑149 (MQ=255)
                                                                                                                                              gacAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGGCGCATCTGTCATACCGCCtt  >  8:257178/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GCGGGACCGGGCGGTGCAGATGGGCAGCACATTGGCCTTGACCATGAAGCTGGAAGACCAGATCCATCCCTGAGTATTTCTCTTCAGTGCTGACTGAAAAATGAACAACATTTAACCAGGCTATCCGTCGCCCCCTGTTGACGACAGGGGGATGTCGGGGCGTATATTGATAGTTAGCAAACTATGAATATACTTGGTTCTCTTCATGTCCCTTGATTTCCTGCGCCTGCAAGTCAGCAGTGGCCTGGTAGTCGCTGCCCGCCACTGGCGGCGCATCTGTCATACCGCCTT  >  NC_002947/226521‑226811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑