| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,367,411 | G→T | 14.3% | V77L (GTG→TTG) | gdhB → | NAD‑specific glutamate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,367,411 | 0 | G | T | 14.3% | 78.0 / 8.0 | 35 | V77L (GTG→TTG) | gdhB | NAD‑specific glutamate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (14/16); new base T (3/2); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-02 | |||||||||||
CCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTG > NC_002947/2367270‑2367554 | ccACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCgg < 5:282328/147‑1 (MQ=255) aGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCGGgtattcg < 8:239876/149‑6 (MQ=255) tGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAACACCCGCACTTGCGGgtattcggg < 1:301110/149‑8 (MQ=255) gcgcTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAAccc > 1:181525/1‑149 (MQ=255) gTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑t > 4:8675/1‑148 (MQ=255) ttCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑t < 1:236942/147‑1 (MQ=255) ttCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑t < 5:15344/147‑1 (MQ=255) ttCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTa < 3:326797/149‑1 (MQ=255) gAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACg > 5:24255/1‑149 (MQ=255) aGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGGACCCTGTCAGCCTTGCGCATCATCGAGCGTTTAGCCCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAAt > 6:275528/1‑149 (MQ=255) gTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAAtg > 2:331822/1‑149 (MQ=255) ggCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCa < 2:181525/149‑1 (MQ=255) aCCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAg < 1:331822/148‑1 (MQ=255) aCCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAg < 3:8675/148‑1 (MQ=255) cTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGa < 5:227048/149‑1 (MQ=255) tttCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGAc > 3:161086/1‑149 (MQ=255) ccGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATAGCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTg < 7:167853/149‑1 (MQ=255) ccTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAAAGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTccg > 7:29869/1‑147 (MQ=255) cTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGt < 6:24255/149‑1 (MQ=255) cgcATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCACTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCt < 5:275528/149‑1 (MQ=255) tcGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGAGTTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAAccg > 1:81399/1‑149 (MQ=255) cGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTgc < 7:323447/131‑1 (MQ=255) cGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTgc > 8:323447/1‑131 (MQ=255) cGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAAccgcc > 7:86022/1‑149 (MQ=255) gAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTg > 4:139436/1‑117 (MQ=255) gAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTg < 3:139436/117‑1 (MQ=255) tttCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGc > 2:303466/1‑148 (MQ=255) tCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACCAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTa < 2:311937/148‑1 (MQ=255) ccccGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGc > 8:268884/1‑148 (MQ=255) ttgtacaCCCGCACTTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCCCCGTGGCTACAGCATc > 3:97188/7‑149 (MQ=255) ttgtacaCCCGCACTTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTACACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATc > 8:14610/7‑149 (MQ=255) tACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCacac < 4:161086/149‑1 (MQ=255) caCCCGCACTTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCccac > 7:155424/2‑149 (MQ=255) cccGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACAccc > 6:217950/1‑149 (MQ=255) cGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTg < 8:86022/149‑1 (MQ=255) | CCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGA‑TTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTG > NC_002947/2367270‑2367554 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |