| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,372,081 | G→T | 10.8% | intergenic (+33/+79) | gdhB → / ← PP_2081 | NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,372,081 | 0 | G | T | 10.8% | 138.4 / 7.4 | 53 | intergenic (+33/+79) | gdhB/PP_2081 | NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein |
| Reads supporting (aligned to +/- strand): ref base G (24/23); new base T (2/4); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01 | |||||||||||
CCATGCTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGC > NC_002947/2371933‑2372229 | ccATGCTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAg < 6:42529/149‑1 (MQ=255) cATGCTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGCGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAg < 1:81151/149‑1 (MQ=255) gCTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GAt > 4:157585/1‑149 (MQ=255) acgacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCgg < 3:9867/149‑1 (MQ=255) cgacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCgg < 2:154055/148‑1 (MQ=255) gacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCgggg < 8:49505/149‑1 (MQ=255) aaTGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCtttttttt < 4:12967/149‑1 (MQ=255) ccACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTAt > 2:318254/1‑149 (MQ=255) cACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGGCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCTG‑CATC‑ACTGCCGGGGCtttgttttacct < 2:114964/148‑5 (MQ=38) aCGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGACGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CATC‑ACTGCCGGGGc > 3:333003/1‑135 (MQ=255) aCGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGACGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CATC‑ACTGCCGGGGc < 4:333003/135‑1 (MQ=255) gCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTgg > 1:252992/1‑149 (MQ=255) aCTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTgggcgg < 2:252992/147‑1 (MQ=255) tGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCAtt > 4:40328/1‑149 (MQ=255) gATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcg > 4:317857/1‑149 (MQ=255) gATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcg > 5:168265/1‑149 (MQ=255) aTGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcgc < 5:109737/149‑1 (MQ=255) gTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGTTGGTAGATGCCGGGGCTTTTTTTTGTATGACTGGGCGGGCCATTTcgc > 1:115765/1‑149 (MQ=255) tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCgg < 8:202904/148‑1 (MQ=255) tACGAGGTTGACAACGTCGAGCTGGTCGACCTGTCATTGTGCGGGCAGGCAGCG‑GTGGTGCCTTACTGAGCCTTGTAGTGATGTAATACAATGGCCCGG‑CATT‑GATGCCGGGGCTTTTTTTTGTTTTCCTGTGCTGGCCATTTcgctg < 8:174936/148‑3 (MQ=255) gcgGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTg > 8:97663/1‑148 (MQ=255) gcgGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTg > 5:218705/1‑148 (MQ=255) cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGTG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTc > 6:193670/1‑149 (MQ=255) cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTc > 8:102416/1‑149 (MQ=255) aCCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTcc > 7:248811/1‑148 (MQ=255) gcgAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGAGGGTGAACACTCTCATACa > 8:313240/1‑149 (MQ=255) gCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCGG‑CATC‑ACTGCCGGGGc > 4:68574/1‑94 (MQ=38) gCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCGG‑CATC‑ACTGCCGGGGc < 3:68574/94‑1 (MQ=38) cTGGTGGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAggggg < 6:326132/149‑1 (MQ=255) aCCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCtttttttt < 6:277608/94‑1 (MQ=255) aCCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCtttttttt > 5:277608/1‑94 (MQ=255) ccATTAGCGGGCAGGCAGGG‑GGGGTGCCGTCCTTAGCCTTGTATTTATGTAAAACAAAGCCCCGG‑CAGT‑TATGCCGGTGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTTAACCCGCTCCTACAGGGGGGGCGGGGCTGTTa < 7:313240/149‑1 (MQ=255) cttGAGGGGGCTGGCTGGG‑GTGGTGCCGTCCTGTGCCTTGTTGTGAGGTAAAACAAAGCCCCGG‑CTGT‑GATGCCGGGGCTTTTTTTTGTTTGCCTGGGCGGGCCTTTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTAc < 2:115765/147‑1 (MQ=38) cATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTAc > 3:193710/1‑149 (MQ=255) tgcgCGGGCTGGCAGGG‑TTGGTGCCGTCCTGAGCGTTGTAGTGAGGTAAACCAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACtt < 8:251765/146‑1 (MQ=39) tGAGCGGGCAGCCAGCG‑GTGGTGCCGTCCTTAGCCTTGTAGTGAGGAAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACtt < 4:193710/149‑1 (MQ=255) gCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGaa < 8:248811/149‑1 (MQ=255) gCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGaa < 3:317857/149‑1 (MQ=255) gggCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcgc < 8:68728/104‑1 (MQ=255) gggCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcgc > 7:68728/1‑104 (MQ=255) gcagCG‑GGGGGTGCGGCCTGATCCGTGTCGTGAGTTAAAACAACGCCCCGG‑CAGT‑GATGCCGGGTCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctct < 6:218705/149‑1 (MQ=37) agCG‑GTGGTGCCGTGCTGAGCCTTGTAGTTAGTTAAAACAAAGCCCGGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTcc < 6:168265/149‑1 (MQ=255) agCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctctc > 3:285456/1‑148 (MQ=255) gCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCa > 2:300119/1‑149 (MQ=255) gCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCtttt > 7:233803/1‑149 (MQ=255) ccGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCttttt < 4:285456/149‑1 (MQ=255) gTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTc > 5:107695/1‑148 (MQ=255) aGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCa < 3:157585/147‑1 (MQ=255) gTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGt < 6:118640/99‑1 (MQ=255) gTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGt > 5:118640/1‑99 (MQ=255) aCAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCtt > 7:239074/1‑149 (MQ=255) cAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACCGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTc < 7:97663/149‑1 (MQ=255) cAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTc < 3:40328/149‑1 (MQ=255) ccccGG‑CAGT‑GATGCCGGGGCGTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCCCGCCGTTTTCGCCCAGGATCTTGGCGGAAAGCGCGTCCAcc > 2:265942/1‑148 (MQ=38) aGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATg > 5:42061/1‑149 (MQ=255) gT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGc < 1:300119/149‑1 (MQ=255) | CCATGCTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCG‑GTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGC > NC_002947/2371933‑2372229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |