Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,372,083	G→A	9.4%	intergenic (+35/+77)	gdhB → / ← PP_2081	NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,372,083	0	G	A	9.4%	140.0 / 4.5	54	intergenic (+35/+77)	gdhB/PP_2081	NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein
Reads supporting (aligned to +/- strand):  ref base G (24/24);  new base A (2/3);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

GCTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGC  >  NC_002947/2371937‑2372229                                                                                                                                                     |                                                                                                                                                   gCTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GAt                                                                                                                                                  >  4:157585/1‑149 (MQ=255)      acgacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCgg                                                                                                                                             <  3:9867/149‑1 (MQ=255)       cgacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCgg                                                                                                                                             <  2:154055/148‑1 (MQ=255)        gacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCgggg                                                                                                                                           <  8:49505/149‑1 (MQ=255)                 aaTGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCtttttttt                                                                                                                                  <  4:12967/149‑1 (MQ=255)                     ccACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTAt                                                                                                                              >  2:318254/1‑149 (MQ=255)                      cACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGGCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCTG‑CATC‑ACTGCCGGGGCtttgttttacct                                                                                                                              <  2:114964/148‑5 (MQ=38)                       aCGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGACGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CATC‑ACTGCCGGGGc                                                                                                                                          <  4:333003/135‑1 (MQ=255)                       aCGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGACGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CATC‑ACTGCCGGGGc                                                                                                                                          >  3:333003/1‑135 (MQ=255)                           gCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTgg                                                                                                                        >  1:252992/1‑149 (MQ=255)                                 aCTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTgggcgg                                                                                                                    <  2:252992/147‑1 (MQ=255)                                     tGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCAtt                                                                                                              >  4:40328/1‑149 (MQ=255)                                        gATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcg                                                                                                           >  5:168265/1‑149 (MQ=255)                                        gATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcg                                                                                                           >  4:317857/1‑149 (MQ=255)                                         aTGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcgc                                                                                                          <  5:109737/149‑1 (MQ=255)                                           gTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGTTGGTAGATGCCGGGGCTTTTTTTTGTATGACTGGGCGGGCCATTTcgc                                                                                                          >  1:115765/1‑149 (MQ=255)                                            tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCgg                                                                                                        <  8:202904/148‑1 (MQ=255)                                            tACGAGGTTGACAACGTCGAGCTGGTCGACCTGTCATTGTGCGGGCAGGCAGCGGTGGTGCCTTACTGAGCCTTGTAGTGATGTAATACAATGGCCCGG‑CATT‑GATGCCGGGGCTTTTTTTTGTTTTCCTGTGCTGGCCATTTcgctg                                                                                                        <  8:174936/148‑3 (MQ=255)                                               gcgGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTg                                                                                                     >  8:97663/1‑148 (MQ=255)                                               gcgGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTg                                                                                                     >  5:218705/1‑148 (MQ=255)                                                       cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGTGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTc                                                                                            >  6:193670/1‑149 (MQ=255)                                                       cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTc                                                                                            >  8:102416/1‑149 (MQ=255)                                                         aCCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTcc                                                                                           >  7:248811/1‑148 (MQ=255)                                                            gcgAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGAGGGTGAACACTCTCATACa                                                                                       >  8:313240/1‑149 (MQ=255)                                                                gCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCGG‑CATC‑ACTGCCGGGGc                                                                                                                                          <  3:68574/94‑1 (MQ=38)                                                                gCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCGG‑CATC‑ACTGCCGGGGc                                                                                                                                          >  4:68574/1‑94 (MQ=38)                                                                 cTGGTGGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAggggg                                                                                  <  6:326132/149‑1 (MQ=255)                                                                        aCCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCtttttttt                                                                                                                                  >  5:277608/1‑94 (MQ=255)                                                                        aCCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCtttttttt                                                                                                                                  <  6:277608/94‑1 (MQ=255)                                                                              ccATTAGCGGGCAGGCAGGGGGGGTGCCGTCCTTAGCCTTGTATTTATGTAAAACAAAGCCCCGG‑CAGT‑TATGCCGGTGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTTAACCCGCTCCTACAGGGGGGGCGGGGCTGTTa                                                                     <  7:313240/149‑1 (MQ=255)                                                                               cttGAGGGGGCTGGCTGGGGTGGTGCCGTCCTGTGCCTTGTTGTGAGGTAAAACAAAGCCCCGG‑CTGT‑GATGCCGGGGCTTTTTTTTGTTTGCCTGGGCGGGCCTTTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTAc                                                                    <  2:115765/147‑1 (MQ=38)                                                                               cATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTAc                                                                    >  3:193710/1‑149 (MQ=255)                                                                                 tgcgCGGGCTGGCAGGGTTGGTGCCGTCCTGAGCGTTGTAGTGAGGTAAACCAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACtt                                                                  <  8:251765/146‑1 (MQ=39)                                                                                 tGAGCGGGCAGCCAGCGGTGGTGCCGTCCTTAGCCTTGTAGTGAGGAAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACtt                                                                  <  4:193710/149‑1 (MQ=255)                                                                                    gCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGaa                                                               <  8:248811/149‑1 (MQ=255)                                                                                    gCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGaa                                                               <  3:317857/149‑1 (MQ=255)                                                                                      gggCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcgc                                                                                                          <  8:68728/104‑1 (MQ=255)                                                                                      gggCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcgc                                                                                                          >  7:68728/1‑104 (MQ=255)                                                                                            gcagCGGGGGGTGCGGCCTGATCCGTGTCGTGAGTTAAAACAACGCCCCGG‑CAGT‑GATGCCGGGTCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctct                                                       <  6:218705/149‑1 (MQ=37)                                                                                              agCGGTGGTGCCGTGCTGAGCCTTGTAGTTAGTTAAAACAAAGCCCGGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTcc                                                     <  6:168265/149‑1 (MQ=255)                                                                                              agCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctctc                                                      >  3:285456/1‑148 (MQ=255)                                                                                               gCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCa                                                    >  2:300119/1‑149 (MQ=255)                                                                                                       gCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCtttt                                            >  7:233803/1‑149 (MQ=255)                                                                                                        ccGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCttttt                                           <  4:285456/149‑1 (MQ=255)                                                                                                          gTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTc                                          >  5:107695/1‑148 (MQ=255)                                                                                                                aGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCa                                     <  3:157585/147‑1 (MQ=255)                                                                                                                              gTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGt                                                                       <  6:118640/99‑1 (MQ=255)                                                                                                                              gTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGt                                                                       >  5:118640/1‑99 (MQ=255)                                                                                                                                   aCAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCtt                >  7:239074/1‑149 (MQ=255)                                                                                                                                    cAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACCGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTc               <  7:97663/149‑1 (MQ=255)                                                                                                                                    cAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTc               <  3:40328/149‑1 (MQ=255)                                                                                                                                         ccccGG‑CAGT‑GATGCCGGGGCGTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCCCGCCGTTTTCGCCCAGGATCTTGGCGGAAAGCGCGTCCAcc           >  2:265942/1‑148 (MQ=38)                                                                                                                                                 aGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATg   >  5:42061/1‑149 (MQ=255)                                                                                                                                                  gT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGc  <  1:300119/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGC  >  NC_002947/2371937‑2372229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑