| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,372,084 | A→C | 9.3% | intergenic (+36/+76) | gdhB → / ← PP_2081 | NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,372,084 | 0 | A | C | 9.3% | 138.1 / 7.5 | 54 | intergenic (+36/+76) | gdhB/PP_2081 | NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein |
| Reads supporting (aligned to +/- strand): ref base A (24/25); new base C (2/3); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01 | |||||||||||
GCTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGCGT > NC_002947/2371937‑2372231 | gCTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GAt > 4:157585/1‑149 (MQ=255) acgacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCgg < 3:9867/149‑1 (MQ=255) cgacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCgg < 2:154055/148‑1 (MQ=255) gacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCgggg < 8:49505/149‑1 (MQ=255) aaTGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCtttttttt < 4:12967/149‑1 (MQ=255) ccACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTAt > 2:318254/1‑149 (MQ=255) cACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGGCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCTG‑CATC‑ACTGCCGGGGCtttgttttacct < 2:114964/148‑5 (MQ=38) aCGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGACGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CATC‑ACTGCCGGGGc < 4:333003/135‑1 (MQ=255) aCGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGACGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CATC‑ACTGCCGGGGc > 3:333003/1‑135 (MQ=255) gCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTgg > 1:252992/1‑149 (MQ=255) aCTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTgggcgg < 2:252992/147‑1 (MQ=255) tGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCAtt > 4:40328/1‑149 (MQ=255) gATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcg > 5:168265/1‑149 (MQ=255) gATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcg > 4:317857/1‑149 (MQ=255) aTGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcgc < 5:109737/149‑1 (MQ=255) gTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGTTGGTAGATGCCGGGGCTTTTTTTTGTATGACTGGGCGGGCCATTTcgc > 1:115765/1‑149 (MQ=255) tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCgg < 8:202904/148‑1 (MQ=255) tACGAGGTTGACAACGTCGAGCTGGTCGACCTGTCATTGTGCGGGCAGGCAGCGGTGGTGCCTTACTGAGCCTTGTAGTGATGTAATACAATGGCCCGG‑CATT‑GATGCCGGGGCTTTTTTTTGTTTTCCTGTGCTGGCCATTTcgctg < 8:174936/148‑3 (MQ=255) gcgGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTg > 5:218705/1‑148 (MQ=255) gcgGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTg > 8:97663/1‑148 (MQ=255) cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGTGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTc > 6:193670/1‑149 (MQ=255) cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTc > 8:102416/1‑149 (MQ=255) aCCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTcc > 7:248811/1‑148 (MQ=255) gcgAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGAGGGTGAACACTCTCATACa > 8:313240/1‑149 (MQ=255) gCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCGG‑CATC‑ACTGCCGGGGc < 3:68574/94‑1 (MQ=38) gCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCGG‑CATC‑ACTGCCGGGGc > 4:68574/1‑94 (MQ=38) cTGGTGGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAggggg < 6:326132/149‑1 (MQ=255) aCCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCtttttttt < 6:277608/94‑1 (MQ=255) aCCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCtttttttt > 5:277608/1‑94 (MQ=255) ccATTAGCGGGCAGGCAGGGGGGGTGCCGTCCTTAGCCTTGTATTTATGTAAAACAAAGCCCCGG‑CAGT‑TATGCCGGTGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTTAACCCGCTCCTACAGGGGGGGCGGGGCTGTTa < 7:313240/149‑1 (MQ=255) cttGAGGGGGCTGGCTGGGGTGGTGCCGTCCTGTGCCTTGTTGTGAGGTAAAACAAAGCCCCGG‑CTGT‑GATGCCGGGGCTTTTTTTTGTTTGCCTGGGCGGGCCTTTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTAc < 2:115765/147‑1 (MQ=38) cATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTAc > 3:193710/1‑149 (MQ=255) tgcgCGGGCTGGCAGGGTTGGTGCCGTCCTGAGCGTTGTAGTGAGGTAAACCAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACtt < 8:251765/146‑1 (MQ=39) tGAGCGGGCAGCCAGCGGTGGTGCCGTCCTTAGCCTTGTAGTGAGGAAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACtt < 4:193710/149‑1 (MQ=255) gCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGaa < 8:248811/149‑1 (MQ=255) gCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGaa < 3:317857/149‑1 (MQ=255) gggCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcgc > 7:68728/1‑104 (MQ=255) gggCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcgc < 8:68728/104‑1 (MQ=255) gcagCGGGGGGTGCGGCCTGATCCGTGTCGTGAGTTAAAACAACGCCCCGG‑CAGT‑GATGCCGGGTCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctct < 6:218705/149‑1 (MQ=37) agCGGTGGTGCCGTGCTGAGCCTTGTAGTTAGTTAAAACAAAGCCCGGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTcc < 6:168265/149‑1 (MQ=255) agCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctctc > 3:285456/1‑148 (MQ=255) gCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCa > 2:300119/1‑149 (MQ=255) gCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCtttt > 7:233803/1‑149 (MQ=255) ccGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCttttt < 4:285456/149‑1 (MQ=255) gTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTc > 5:107695/1‑148 (MQ=255) aGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCa < 3:157585/147‑1 (MQ=255) gTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGt < 6:118640/99‑1 (MQ=255) gTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGt > 5:118640/1‑99 (MQ=255) aCAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCtt > 7:239074/1‑149 (MQ=255) cAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACCGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTc < 7:97663/149‑1 (MQ=255) cAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTc < 3:40328/149‑1 (MQ=255) ccccGG‑CAGT‑GATGCCGGGGCGTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCCCGCCGTTTTCGCCCAGGATCTTGGCGGAAAGCGCGTCCAcc > 2:265942/1‑148 (MQ=38) aGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATg > 5:42061/1‑149 (MQ=255) gT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGc < 1:300119/149‑1 (MQ=255) aTGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGCGt < 7:102416/148‑1 (MQ=255) aTGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGCGt < 6:107695/148‑1 (MQ=255) | GCTCGACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGG‑CAGT‑GATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGCGT > NC_002947/2371937‑2372231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |