Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,395,583 G→C 7.5% intergenic (‑191/+52) dacB ← / ← PP_2099 D‑alanyl‑D‑alanine carboxypeptidase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,395,5830GC 7.5% 159.2 / 2.6 53intergenic (‑191/+52)dacB/PP_2099D‑alanyl‑D‑alanine carboxypeptidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base C (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GAGTGAATACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCC  >  NC_002947/2395438‑2395726
                                                                                                                                                 |                                                                                                                                               
gAGTGAATACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAAt                                                                                                                                              >  3:322462/1‑149 (MQ=255)
   tGAATACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATggg                                                                                                                                           <  5:122945/149‑1 (MQ=255)
     aaTACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGt                                                                                                                                         >  2:91895/1‑149 (MQ=255)
         cccTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGcc                                                                                                                                     <  7:124635/149‑1 (MQ=255)
          ccTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCt                                                                                                                                    >  7:92168/1‑149 (MQ=255)
           cTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCtt                                                                                                                                   <  7:165782/149‑1 (MQ=255)
              cggcgAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCCATTCGGGTGGCCtttt                                                                                                                                 <  5:297821/148‑1 (MQ=255)
                  gAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCCATTCGGGTGGCCtttttattg                                                                                                                            >  8:207997/1‑144 (MQ=255)
                  gAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCCATTCGGGTGGCCtttttattg                                                                                                                            <  7:207997/149‑6 (MQ=255)
                       aaaaGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCaa                                                                                                                       >  5:68680/1‑149 (MQ=255)
                       aaaaGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCaa                                                                                                                       >  4:184272/1‑149 (MQ=255)
                            ggCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACCGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCttt                                                                                                                  <  5:273280/149‑1 (MQ=255)
                                     gCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGaa                                                                                                         >  4:249363/1‑149 (MQ=255)
                                      cTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAg                                                                                                        <  3:291736/149‑1 (MQ=255)
                                        gTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCt                                                                                                      >  3:131877/1‑149 (MQ=255)
                                         tAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCTCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCt                                                                                                      >  1:241386/1‑148 (MQ=255)
                                          aaGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGa                                                                                                    >  3:155898/1‑149 (MQ=255)
                                          aaGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGCTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGa                                                                                                    >  7:181179/1‑149 (MQ=255)
                                              gatgatGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACaa                                                                                                 <  3:249363/148‑1 (MQ=255)
                                                tgatgGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTa                                                                                              <  4:131877/149‑1 (MQ=255)
                                                  atgGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTAt                                                                                             <  1:187323/148‑1 (MQ=255)
                                                  atgGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTAt                                                                                             >  4:68939/1‑148 (MQ=255)
                                                    ggCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCAc                                                                                          >  4:155236/1‑149 (MQ=255)
                                                      ccccACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCt                                                                                                                                    >  6:185027/1‑105 (MQ=255)
                                                      ccccACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCt                                                                                                                                    <  5:185027/105‑1 (MQ=255)
                                                       cccACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACt                                                                                         >  8:238835/1‑147 (MQ=255)
                                                           cgacgTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCt                                                                                   <  4:155898/149‑1 (MQ=255)
                                                             acgTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCttg                                                                                 <  7:238835/149‑1 (MQ=255)
                                                              cgTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCttgt                                                                                <  3:282426/149‑1 (MQ=255)
                                                               gTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCttgtt                                                                               <  4:322462/149‑1 (MQ=255)
                                                               gTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCttgtt                                                                               <  3:68939/149‑1 (MQ=255)
                                                                tAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCttgtt                                                                               >  7:38508/1‑148 (MQ=255)
                                                                   aCAGTACGCATTATGCCCCAAACGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTTACAAGTATCACTCAGCCTTGTTGATa                                                                           <  6:320936/149‑1 (MQ=255)
                                                                            aTTATGCCCCAAGCGCAGCCGCGATGTGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTcc                                                                  >  7:4004/1‑149 (MQ=255)
                                                                                            aGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCca                                                  <  2:241386/149‑1 (MQ=255)
                                                                                               ctcgATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATTGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCcagca                                               <  6:68680/147‑1 (MQ=255)
                                                                                                   aTGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCttgtt                                                                               >  7:308385/1‑113 (MQ=255)
                                                                                                   aTGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCttgtt                                                                               <  8:308385/113‑1 (MQ=255)
                                                                                                    tGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCggg                                          <  3:155236/149‑1 (MQ=255)
                                                                                                         cACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGAc                                     <  3:184272/149‑1 (MQ=255)
                                                                                                             ttCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTc                                  >  4:292318/1‑148 (MQ=255)
                                                                                                                 aCGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGATGACTTCGATCt                             >  4:250004/1‑149 (MQ=255)
                                                                                                                   ggAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCAt                                                     <  7:18176/123‑1 (MQ=255)
                                                                                                                   ggAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCAt                                                     >  8:18176/1‑123 (MQ=255)
                                                                                                                      aaaGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGt                         >  4:60687/1‑148 (MQ=255)
                                                                                                                      aaaGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTc                        <  3:60687/149‑1 (MQ=255)
                                                                                                                            tCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCg                  >  2:21329/1‑149 (MQ=255)
                                                                                                                                 aaTAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCttgt                                                                                >  7:266620/1‑82 (MQ=255)
                                                                                                                                 aaTAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCttgt                                                                                <  8:266620/82‑1 (MQ=255)
                                                                                                                                        aGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTg      <  8:181179/149‑1 (MQ=255)
                                                                                                                                         ggCCACCCCATTCGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCttt       >  2:223595/1‑147 (MQ=255)
                                                                                                                                            cACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGcc  <  8:4004/149‑1 (MQ=255)
                                                                                                                                             aCCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGc   >  6:191979/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GAGTGAATACCCTGCGGCGAGGGAAAAGGGCTTTGGGGCTGTAAGAGATGATGGCCCCACGACGTAAACAGTACGCATTATGCCCCAAGCGCAGCCGCGATGCGTCACGTTCGACGGAAAAGCATCGCCAATAAAAAGGCCACCCGAATGGGGTGGCCTTTTCAGCAATCAACCTTTCAGCCCGAAGCTGACAAGTATCACTCAGCCTTGTTGATAGGCTTTTCCGGGTACCAGGCATCCAGCAGCGGGCTGACTTCGATCTTGGTCAGTTCGCTGCGGCCTTTGAGCC  >  NC_002947/2395438‑2395726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑