| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,412,905 | G→C | 13.1% | intergenic (+169/‑252) | rlmM → / → PP_2114 | 23S rRNA (cytidine(2498)‑2'‑O)‑methyltransferase RlmM/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,412,905 | 0 | G | C | 13.1% | 108.2 / 9.6 | 46 | intergenic (+169/‑252) | rlmM/PP_2114 | 23S rRNA (cytidine(2498)‑2'‑O)‑methyltransferase RlmM/transposase |
| Reads supporting (aligned to +/- strand): ref base G (23/17); new base C (3/3); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
TCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGC > NC_002947/2412769‑2413047 | tctgcttctgctTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATCCATGGGGCCt < 6:314325/146‑1 (MQ=255)tctgcttctgctTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATCCATGGGGCCt > 5:314325/1‑146 (MQ=255) cttctgcttTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATCCATGGGGCCtcagc < 5:140555/146‑5 (MQ=255) ttttGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATCCATGGGGCCtcag < 8:5063/138‑4 (MQ=255) ttttGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATCCATGGGGCCtcag > 7:5063/1‑135 (MQ=255) gCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCgg > 2:82197/1‑148 (MQ=37) cTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCggg < 8:38555/148‑1 (MQ=37) gTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTCCAACGATTACTCACgtcag > 6:239623/1‑144 (MQ=11) ttGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGc > 2:33324/1‑148 (MQ=34) gCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCggg > 1:91142/1‑143 (MQ=34) gCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCggg < 2:91142/143‑1 (MQ=34) cgATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAggg < 3:282114/149‑1 (MQ=37) gATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCg < 2:249531/144‑1 (MQ=37) gATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCg > 1:249531/1‑144 (MQ=37) gCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGc < 6:157098/149‑1 (MQ=37) gACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCg < 6:47783/132‑1 (MQ=37) gACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCg > 5:47783/1‑132 (MQ=37) gCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAgc > 8:203677/1‑149 (MQ=37) aGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAgcg > 5:36539/1‑148 (MQ=37) ggCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAg > 6:96533/1‑148 (MQ=37) gCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGt < 5:163975/126‑1 (MQ=38) gCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGt > 6:163975/1‑126 (MQ=38) gCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCa < 5:96533/149‑1 (MQ=39) ttGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGtttttt > 2:242542/1‑149 (MQ=39) ttGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCACTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGtttttt > 6:351469/1‑149 (MQ=255) ggTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACttttt > 2:183392/1‑149 (MQ=39) ggTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACttttt < 1:33324/149‑1 (MQ=39) ttGACGGGCATCCATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTg > 4:24091/2‑149 (MQ=255) gTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTg > 8:232929/1‑149 (MQ=39) gTGACGGGCATGGATGCCCGTCAAGCACTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTg < 5:351469/149‑1 (MQ=255) gCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTcaaacg > 1:244559/1‑144 (MQ=255) cATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGttt > 3:164906/1‑149 (MQ=39) gATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGAc > 1:42490/1‑148 (MQ=39) cccGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACaaaaa < 1:183392/149‑1 (MQ=39) cGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGt < 1:82197/149‑1 (MQ=39) cGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGt > 8:40682/1‑149 (MQ=39) aaGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGttt < 6:101498/135‑1 (MQ=40) aaGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGttt > 5:101498/1‑135 (MQ=40) gcgcTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGga > 6:115826/1‑91 (MQ=37) gcgcTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGga < 5:115826/91‑1 (MQ=37) gcTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACccgcc < 2:42490/149‑1 (MQ=39) gCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAgg < 7:40682/149‑1 (MQ=39) gCCCCATCCATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAgg > 5:61594/1‑149 (MQ=255) cccATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGc < 6:225587/77‑1 (MQ=39) cccATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGc > 5:225587/1‑77 (MQ=39) ccATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGc > 2:97261/1‑148 (MQ=39) | TCTGCTTCTGCTTTTGCTGTTGCTTCTAAGCGCGCGATAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGTAGGGCTTGCGAAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGTACTCCCGGGAGCAAGCCCGGAGCGAGGGAACCCCGGAGCGTAGCGCAGGGGCCGGATGATAGGAGCGCAGCGTTTTTTGGTTACTTTTTGTCGCGTTTGACAAAAAGTGACCCGCCGTAAGGGC > NC_002947/2412769‑2413047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |