Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,456,057 T→A 12.2% C258S (TGC→AGC) ‡ gapB → glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,456,0570TA12.2% 121.8 / 7.2 49C258S (TGC→AGC) ‡gapBglyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (26/17);  new base A (2/4);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

CATCACTGCCAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCGTGCCGGTG  >  NC_002947/2455909‑2456203
                                                                                                                                                    |                                                                                                                                                  
cATCACTGCCAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCt                                                                                                                                                    >  7:173330/1‑149 (MQ=255)
       gCCAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCggg                                                                                                                                             >  6:344327/1‑149 (MQ=255)
        ccAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCTACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCgg                                                                                                                                              <  8:306575/147‑1 (MQ=255)
        ccAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCgg                                                                                                                                              <  1:27511/147‑1 (MQ=255)
         cAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGtg                                                                                                                                           >  3:113577/1‑149 (MQ=255)
          aaCGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGtgc                                                                                                                                          >  6:116560/1‑149 (MQ=255)
          aaCGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCCGGGCAGGCCAGGtgc                                                                                                                                          <  6:24842/149‑1 (MQ=255)
             ctcAACCTCATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCCGGGCAGGCCAGGtgctgg                                                                                                                                       <  7:307605/147‑2 (MQ=255)
                aaCCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCCGGGCAGGCCAGGtgctgg                                                                                                                                       >  8:307605/1‑145 (MQ=255)
                aaCCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCCGGGCAGGCCAGGtgctgg                                                                                                                                       >  8:303167/1‑145 (MQ=255)
                aaCCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCCGGGCAGGCCAGGtgctgg                                                                                                                                       <  7:303167/146‑2 (MQ=255)
                  ccTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGG‑CAGCACCCGGGCAGGCCAGGTGCTGgcccagg                                                                                                                                 <  2:103773/149‑5 (MQ=38)
                             ttATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCAcc                                                                                                                       >  7:261392/1‑149 (MQ=255)
                                 cTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCAc                                                                                                                   >  4:198404/1‑149 (MQ=255)
                                    cGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCAc                                                                                                                   <  7:90567/146‑1 (MQ=255)
                                    cGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCAc                                                                                                                   >  8:90567/1‑146 (MQ=255)
                                       cAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGa                                                                                                                               <  7:73022/131‑1 (MQ=255)
                                       cAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGa                                                                                                                               >  8:73022/1‑131 (MQ=255)
                                       cAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAAAGCGCTCATCGACGACAACACCGGCGTAAGCACTGACGCCGACGGCCTGGGCAAGCACCTGGCCTGACAGGGTGCTGCCCGCGGGATCCTCACAGAACCTgga                                                                                                              >  8:98644/1‑147 (MQ=255)
                                            gCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGgc                                                                                                        <  4:113577/149‑1 (MQ=255)
                                                          gACTACACCCAGTACGGCATCGACAAAGCCCTCATCGAAAACAAAAACGACGTATGCCGTGACGACCACCGCCTGGGCCAGCACATGTCCTTCACGGGTGCTGACCGCGTGATCCTCAACCAACATGACAAGGACGCGCTGAAGAACa                                                                                           >  4:309000/1‑148 (MQ=255)
                                                               cacCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATc                                                                                                                             <  4:217371/109‑1 (MQ=255)
                                                               cacCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATc                                                                                                                             >  3:217371/1‑109 (MQ=255)
                                                                acCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCa                                                                                    >  4:266362/1‑149 (MQ=255)
                                                                        cGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATc                                                                             <  3:266362/148‑1 (MQ=255)
                                                                         ggCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCaa                                                                           >  4:38086/1‑149 (MQ=255)
                                                                            aTCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCa                                                                        >  5:32144/1‑149 (MQ=255)
                                                                                aCAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACTTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGt                                                                    >  7:48565/1‑149 (MQ=255)
                                                                                aCAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGt                                                                    >  8:40395/1‑149 (MQ=255)
                                                                                aCAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGt                                                                    <  8:261392/149‑1 (MQ=255)
                                                                                            tcgtcgACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGcc                                                        >  8:263084/1‑149 (MQ=255)
                                                                                                 gACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACTTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGa                                                   <  8:48565/149‑1 (MQ=255)
                                                                                                        ccGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGatc                                            <  7:69024/149‑1 (MQ=255)
                                                                                                         cGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGatca                                           >  4:42170/1‑149 (MQ=255)
                                                                                                           gCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGatcatc                                         >  1:188802/1‑149 (MQ=255)
                                                                                                           gCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGATGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAAACACTGTGATATCAATGCCGATGACAAGataat                                          >  2:253780/1‑148 (MQ=255)
                                                                                                            cGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCAtct                                        <  7:40395/149‑1 (MQ=255)
                                                                                                              tATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTcg                                      >  4:324162/1‑149 (MQ=255)
                                                                                                              tATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTcg                                      <  3:198404/149‑1 (MQ=255)
                                                                                                                    gTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGcc                                >  8:221576/1‑149 (MQ=255)
                                                                                                                     tGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACg                                                                                  <  2:223949/98‑1 (MQ=255)
                                                                                                                     tGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACg                                                                                  >  1:223949/1‑98 (MQ=255)
                                                                                                                         gCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTcggcg                                   <  7:138976/141‑1 (MQ=255)
                                                                                                                         gCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTcggcg                                   >  8:138976/1‑141 (MQ=255)
                                                                                                                             aCGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGcacc                       <  2:188802/149‑1 (MQ=255)
                                                                                                                                          gCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGAAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCg          >  2:73530/1‑149 (MQ=255)
                                                                                                                                            aCCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGAGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCGTg        >  4:292159/1‑149 (MQ=255)
                                                                                                                                              cTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCGTGcc      >  8:238772/1‑149 (MQ=255)
                                                                                                                                              cTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCGTGcc      <  7:238772/149‑1 (MQ=255)
                                                                                                                                                  ccTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCGTGCCGGTg  <  3:324162/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CATCACTGCCAACGGCAACCTGATCCAGGTTATCTACGCCAAGAGCCCTAGCGAAGTCGACTACACCCAGTACGGCATCGACAATGCGCTCATCGTCGACAACACCGGCGTATGGCGTGACGCCGACGGCCTGGGCCAGCACCTGGCCTGCCCGGGTGCTGCCCGCGTGATCCTCACCGCACCTGGCAAGGGCGCGCTGAAGAACATCGTGCACGGTATCAACCACGGTGATATCACTGCCGATGACAAGATCATCTCGGCGGCCTCCTGCACCACCAACGCCATCGTGCCGGTG  >  NC_002947/2455909‑2456203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑