| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,489,534 | C→T | 11.1% | A177A (GCC→GCT) | PP_2184 → | formate dehydrogenase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,489,534 | 0 | C | T | 11.1% | 92.9 / 2.5 | 36 | A177A (GCC→GCT) | PP_2184 | formate dehydrogenase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (14/18); new base T (2/2); total (16/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCGGTTTCGCAGGCCTGGAGGCCGCTGTGGCGCTGGACGGTGCCGAAGTGGTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTGCCGGCCTCGCCGTGGGTGCCGACAAGG > NC_002947/2489390‑2489670 | cggcggTTTCGCAGGCCTGGAGGCCGCTGTGGCGCTGGACGGTGCCGAAGTGGTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCggg > 6:120739/1‑148 (MQ=255) cgttttCGCAGGCCTGGAGGCCGGTGTGGCGCTGGACGGTGCCGAAGTGGTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCTTCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGa < 8:24896/146‑1 (MQ=255) ggTTTCGCAGGCCTGGAGGCCGCTGTGGCGCTGGACGGTGCCGAAGTGGTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGaa < 7:285885/149‑1 (MQ=255) cGCAGGCCTGGAGGCCGCTGTGGCGCTGGACGGTGCCGAAGTGGTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTAc < 4:218360/148‑1 (MQ=255) gCAGGCCTGGAGGCCGCTGTGGCGCTGGACGGTGCCGAAGTGGTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACgt > 6:286122/1‑149 (MQ=255) ggCCTGGAGGCCGCTGTGGCGCTGGACGGTGCCGAAGTGGTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACgtggt < 2:275597/149‑1 (MQ=255) tgGCGCTGGACGGTGCCGAAGTGGTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAg < 2:23987/149‑1 (MQ=255) gAAGTGGTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAgg > 8:279496/1‑133 (MQ=255) gAAGTGGTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAgg < 7:279496/133‑1 (MQ=255) gTGGTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGc < 5:286122/149‑1 (MQ=255) gTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGa < 8:27263/149‑1 (MQ=255) cAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCAt > 2:128048/1‑148 (MQ=255) tCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCTGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATg > 7:275833/1‑149 (MQ=255) tCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCTGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATg > 7:181882/1‑149 (MQ=255) tCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTc > 6:185833/1‑116 (MQ=255) tCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTc < 5:185833/116‑1 (MQ=255) cGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAgg > 3:171647/1‑107 (MQ=255) cGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAgg < 4:171647/107‑1 (MQ=255) ggtggtgCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGAc > 6:162403/1‑135 (MQ=255) ggtggtgCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGAc < 5:162403/135‑1 (MQ=255) gcggcgTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGAc > 6:39058/1‑129 (MQ=255) gcggcgTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGAc < 5:39058/129‑1 (MQ=255) gTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGa > 3:304476/1‑149 (MQ=255) cccGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCa > 4:131264/1‑149 (MQ=255) cggccggaATCAAGTGGCGTAGCGTGCGTGATGCTGGCGCCTGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCAtt < 3:42817/149‑1 (MQ=255) ggccggcATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTg > 4:162299/1‑149 (MQ=255) gccggcATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTGc < 8:237381/149‑1 (MQ=255) aaGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTGCCGGCCTCGc > 8:113256/1‑149 (MQ=255) gCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTGCGGGCCTCGCCGTgg > 7:61074/1‑149 (MQ=255) gCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTGCCGGCCTCGCCGTgg < 7:113256/149‑1 (MQ=255) gCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTGCCGGCCTCGCCGTgg < 8:61074/149‑1 (MQ=255) gTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTGCCGGCCTCGCCGTGGGt > 8:146623/1‑149 (MQ=255) gTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTGCCGGCCTCGCCGTGGGt < 3:131264/149‑1 (MQ=255) gtgcgtgATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTGCCGGCCTCGCCGTGGGTGCCGa < 5:120739/149‑1 (MQ=255) tgATGCTGGCGCTGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTGCCGGCCTCGCCGTGGGTGCCGACAAgg < 8:181882/149‑1 (MQ=255) tgATGCTGGCGCTGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTGCCGGCCTCGCCGTGGGTGCCGACAAgg < 8:275833/149‑1 (MQ=255) | CGGCGGTTTCGCAGGCCTGGAGGCCGCTGTGGCGCTGGACGGTGCCGAAGTGGTTGCCGCAGTACTCGACTCCGGCCTGCGTGGCCGTGGTGGTGCGGCGTTCCCGGCCGGCATCAAGTGGCGTACCGTGCGTGATGCTGGCGCCGGGCAGAAGTACGTGGTGTGCAACGCCGACGAGGGTGACTCGGGCACGTTCGCTGATCGCATGCTGATGGAAGGCGACCCCTTCCTGCTGATCGAGGGCATGATCATTGCCGGCCTCGCCGTGGGTGCCGACAAGG > NC_002947/2489390‑2489670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |