Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,546,498	G→T	8.6%	T533T (ACG→ACT) ‡	PP_2238 →	metallopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,546,498	0	G	T	8.6%	171.3 / 6.3	58	T533T (ACG→ACT) ‡	PP_2238	metallopeptidase
Reads supporting (aligned to +/- strand):  ref base G (22/31);  new base T (2/3);  total (24/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01

GCCGTTGCTCGACGCCATTGCCCGGGCGC‑CGATCTGGGCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGTGACTTCCTGCAGTTCGAAACCTTGA  >  NC_002947/2546351‑2546640                                                                                                                                                     |                                                                                                                                                gCCGTTGCTCGACGCCATTGCCCGGGCGC‑CGATCTGGGCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACgg                                                                                                                                                >  4:187663/1‑149 (MQ=255) gCCGTTGCTCGACGCCATTGCCCGGGCGC‑CGATCTGGGCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACgg                                                                                                                                                <  3:187663/149‑1 (MQ=255)  ccGTTGCTCGACGCCATTGCCCGGGCGCACGATCTGGGCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACgg                                                                                                                                                >  1:196600/1‑149 (MQ=255)        cTCGACGCCATTGCCCGGGCGC‑CGATCTGGGCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCATCGCCGTCTGCGGTGCa                                                                                                                                         <  3:274182/149‑1 (MQ=255)            aCGCCATTGCCCGGGCGC‑CGATCTGGGCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTg                                                                                                                                     <  7:282052/149‑1 (MQ=255)               ccATTGCCCGGGCGC‑CGATCTGGGCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGc                                                                                                                                  <  2:143867/149‑1 (MQ=255)                  ttGCCCGGGCGC‑CGATTTGGGATGAACAGGTCGACTATGGCTATGGTACGGGCCACGGCGTGGGATACTTAATGAACGTGCATGAGGGGACTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGc                                                                                                                                          <  4:144875/138‑1 (MQ=255)                  ttGCCCGGGCGC‑CGATCTGGGCTGACCAGGGCGACTATGGCCATGGAAGCGGCCACTGAGTGGGCTACATCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCAACCAGGCGGCACCTGCACCGCAGACGGCGATGc                                                                                                                                          >  3:144875/1‑138 (MQ=255)                      ccGGGCGC‑CGTTCTTGGCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCa                                                                                                                           <  3:87454/149‑1 (MQ=255)                        gggCGC‑CGATCTGGGCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGGGGGCTACTTCATGAACGTGCATGAGGGGGCTCAAGTGATCGCCTACCAGGCGGCACCTGCCCCGCAGACGGCGATGCAAGTGGGCTTGCTCCGc                                                                                                                         >  4:260412/1‑149 (MQ=255)                               cGATCTGGGCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCtcgatc                                                                                                                   >  5:327061/1‑149 (MQ=255)                                     gggCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCtcga                                                                                                                     <  2:282914/141‑1 (MQ=255)                                     gggCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGCCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCtcga                                                                                                                     >  1:282914/1‑141 (MQ=255)                                      ggCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCtcgatc                                                                                                                   <  6:327061/142‑1 (MQ=255)                                      ggCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCtcgatc                                                                                                                   <  8:185598/142‑1 (MQ=255)                                      ggCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGcc                                                                                                              <  1:104159/147‑1 (MQ=255)                                      ggCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCgg                                                                                                            <  8:181576/149‑1 (MQ=255)                                      ggCGGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCgg                                                                                                            <  2:160610/149‑1 (MQ=255)                                        cTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGgt                                                                                                          <  1:146094/149‑1 (MQ=255)                                             gaGGTCGCCTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGgtacgta                                                                                                     <  4:264771/148‑1 (MQ=255)                                                gTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGCCGGCGAGGCTAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCg                                                                                                  >  2:54284/1‑149 (MQ=255)                                                gTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCg                                                                                                  >  1:140111/1‑149 (MQ=255)                                                    aCTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCg                                                                                              >  3:36001/1‑149 (MQ=255)                                                      tATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCggg                                                                                            >  3:40518/1‑149 (MQ=255)                                                        tGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCgggg                                                                                           <  4:36001/148‑1 (MQ=255)                                                          gCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCtg                                                                                        >  6:181501/1‑149 (MQ=255)                                                           ccATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCtgt                                                                                       <  2:180916/149‑1 (MQ=255)                                                              tGGTACCGGCCACGGCGTGGGCTACTTCATGACCGTGCATGAGGGGCCTCAAGTGCTCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATTATGAGCTCGATCGAGCCGGGTACTTACCGCCCGGGGCTGTggg                                                                                    <  7:307462/149‑1 (MQ=255)                                                                  aCCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCATCGCCGTCTGCGGTGCAGGTgc                                                                                                                                    >  7:222644/1‑96 (MQ=38)                                                                  aCCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCATCGCCGTCTGCGGTGCAGGTgc                                                                                                                                    <  8:222644/97‑2 (MQ=38)                                                                               tGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAAc                                                                   <  5:181501/149‑1 (MQ=255)                                                                                           tGAACGTGCATGACGGGCCTCAAGGGATCGCCTACCCGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAAc                                                      <  3:260412/149‑1 (MQ=255)                                                                                            gAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCg                                                                        <  6:226364/131‑1 (MQ=255)                                                                                            gAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCg                                                                        >  5:226364/1‑131 (MQ=255)                                                                                             aaCGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCtgt                                                                                       <  8:313241/115‑1 (MQ=255)                                                                                             aaCGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCtgt                                                                                       >  7:313241/1‑115 (MQ=255)                                                                                             aaCGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAAcc                                                     >  3:222373/1‑148 (MQ=255)                                                                                               cGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCgag                                                  <  4:14775/149‑1 (MQ=255)                                                                                                     tGAGGGGCCTCAAGTGATCGCCTACCCGGCGGCACCTGCACCGCAGACGGCGATGCAAGTCGGCATGATCAGCTCGATCGAGCCGGGTCCGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCgg                                            <  1:54284/149‑1 (MQ=255)                                                                                                     tGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCgg                                            >  1:224763/1‑149 (MQ=255)                                                                                                       aGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCggg                                           <  3:18037/148‑1 (MQ=255)                                                                                                            ccTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAagag                                     >  4:35515/1‑149 (MQ=255)                                                                                                                      tCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCtt                               >  2:320881/1‑145 (MQ=255)                                                                                                                      tCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCtt                               <  1:320881/145‑1 (MQ=255)                                                                                                                      tCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGt                           <  3:35515/149‑1 (MQ=255)                                                                                                                      gcGCCTACCACGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATCATCAGCTCGAGCGCGCCGGGTACGGACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGt                           <  4:222373/148‑1 (MQ=255)                                                                                                                       cGCCTACCAGGCGGCACCTGCACCGCAGACGTCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGTCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGt                           <  8:136983/148‑1 (MQ=255)                                                                                                                        gCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGTGa                         >  1:192517/1‑149 (MQ=255)                                                                                                                         ccTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTg                             >  5:41337/1‑144 (MQ=255)                                                                                                                         ccTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTg                             <  6:41337/144‑1 (MQ=255)                                                                                                                           tACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCtggtg                                                             <  4:191262/111‑1 (MQ=255)                                                                                                                           tACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCtggtg                                                             >  3:191262/1‑111 (MQ=255)                                                                                                                              cAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTg                             >  8:315715/1‑139 (MQ=255)                                                                                                                              cAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTg                             <  7:315715/139‑1 (MQ=255)                                                                                                                                     cACTTGCATCGCCGTCTGCGGTGCAGGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGt                           >  2:122044/1‑134 (MQ=255)                                                                                                                                     cACTTGCATCGCCGTCTGCGGTGCAGGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGt                           <  1:122044/134‑1 (MQ=255)                                                                                                                                            aCCGCAGACGGCGATGCAAGTGGGCATGATCAGATCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGTGACTTCCTGCAGTTCGAAACCt     >  2:145183/1‑149 (MQ=255)                                                                                                                                              cGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGTGACTTCCTGCAGTTCGAAACCtt    >  8:112375/1‑148 (MQ=255)                                                                                                                                               gCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGTGACTTCCTGCAGTTCGAAACCTTGa  <  4:40518/149‑1 (MQ=255)                                                                                                                                                cAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTGCGTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGt                           <  1:41397/124‑1 (MQ=255)                                                                                                                                                cAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGt                           >  2:41397/1‑123 (MQ=255)                                                                                                                                                     |                                                                                                                                                GCCGTTGCTCGACGCCATTGCCCGGGCGC‑CGATCTGGGCTGACCAGGTCGACTATGGCCATGGTACCGGCCACGGCGTGGGCTACTTCATGAACGTGCATGAGGGGCCTCAAGTGATCGCCTACCAGGCGGCACCTGCACCGCAGACGGCGATGCAAGTGGGCATGATCAGCTCGATCGAGCCGGGTACGTACCGCCCGGGGCTGTGGGGCGTTCGCATCGAGAACCTGGTG‑GTCAACCGAGAAGCGGGGAAGAGTGCCTTTGGTGACTTCCTGCAGTTCGAAACCTTGA  >  NC_002947/2546351‑2546640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑