Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,576,412 A→G 10.0% E530G (GAG→GGG)  PP_2258 → sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,576,4120AG10.0% 103.8 / 3.5 40E530G (GAG→GGG) PP_2258sensory box protein
Reads supporting (aligned to +/- strand):  ref base A (17/19);  new base G (2/2);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GTGCGTGACATCCTTCATGACCAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTG  >  NC_002947/2576270‑2576560
                                                                                                                                              |                                                                                                                                                    
gtgcgtgACATCCTTCATGACCAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTgg                                                                                                                                                 <  3:279716/148‑1 (MQ=255)
     tgACATCCTTCATTAGCAGGACGACCTGGCGCTGACCTAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAg                                                                                                                                            <  4:251266/148‑1 (MQ=255)
        cATCCTTCATGACCAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGt                                                                                                                                         >  5:259135/1‑148 (MQ=255)
            cTTCATGACCAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTg                                                                                                                                    >  6:217485/1‑149 (MQ=255)
                aTGACCAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATc                                                                                                                                <  8:319724/149‑1 (MQ=255)
                 tGACCAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGGGCTGGCGCAGGGTTACTGCATCg                                                                                                                               >  6:136776/1‑149 (MQ=255)
                 tGACCAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGGGCTGGCGCAGGGTTACTGCATCg                                                                                                                               >  6:131601/1‑149 (MQ=255)
                     cAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCg                                                                                                                           >  1:328687/1‑149 (MQ=255)
                       ggACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGc                                                                                                                         <  6:119691/149‑1 (MQ=255)
                              cTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGcc                                                                                                                  >  2:291038/1‑149 (MQ=255)
                                ggCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGAt                                                                                                                     >  6:280338/1‑144 (MQ=255)
                                ggCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGAt                                                                                                                     <  5:280338/144‑1 (MQ=255)
                                           aGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTg                                                                                                     <  6:259135/149‑1 (MQ=255)
                                               aGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCa                                                                                                             >  5:117059/1‑137 (MQ=255)
                                               aGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCa                                                                                                             <  6:117059/137‑1 (MQ=255)
                                                  gATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGc                                                                                                <  3:148722/147‑1 (MQ=255)
                                                  gATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGc                                                                                                >  4:148722/1‑147 (MQ=255)
                                                                        ggCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCagcagc                                                                         <  5:217485/148‑1 (MQ=255)
                                                                              gAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGGCCGGGCGATGca                                                                                                                  <  8:256246/101‑2 (MQ=255)
                                                                              gAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGGCCGGGCGATGca                                                                                                                  >  7:256246/1‑100 (MQ=255)
                                                                                        ccGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGAc                                                        >  7:58425/1‑149 (MQ=255)
                                                                                         cGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGc                                                             <  5:7438/143‑1 (MQ=255)
                                                                                         cGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGc                                                             >  6:7438/1‑143 (MQ=255)
                                                                                         cGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGAcc                                                       >  6:333171/1‑149 (MQ=255)
                                                                                           aGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCt                                                     <  2:328687/149‑1 (MQ=255)
                                                                                           aGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCt                                                     <  3:85221/149‑1 (MQ=255)
                                                                                            ggggCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCa                                                                                                             >  5:53791/1‑92 (MQ=255)
                                                                                            ggggCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCa                                                                                                             <  6:53791/92‑1 (MQ=255)
                                                                                             gggCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCtt                                                    <  8:58425/148‑1 (MQ=255)
                                                                                              ggCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGGGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGa                                                  <  5:136776/149‑1 (MQ=255)
                                                                                              ggCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGGGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGa                                                  <  5:131601/149‑1 (MQ=255)
                                                                                               gCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGaa                                                 <  6:330767/149‑1 (MQ=255)
                                                                                                 tGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGc                                                                                          >  7:291258/1‑106 (MQ=255)
                                                                                                 tGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGc                                                                                          <  8:291258/106‑1 (MQ=255)
                                                                                                 tGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCACCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAgg                                               >  7:66840/1‑149 (MQ=255)
                                                                                                          tCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCg                                      >  5:29064/1‑149 (MQ=255)
                                                                                                                 cGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTg                               <  5:333171/149‑1 (MQ=255)
                                                                                                                                   ccTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACgggg             >  6:97854/1‑149 (MQ=255)
                                                                                                                                      gggCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGgc          >  8:184332/1‑149 (MQ=255)
                                                                                                                                      gggCTGAGAGCTGGCGCAGGGTTACTGCATCACCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGgc          <  8:66840/149‑1 (MQ=255)
                                                                                                                                              aGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTg  >  6:13970/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GTGCGTGACATCCTTCATGACCAGGACGACCTGGCGCTGACGGAGGCAGTGATCGGCCTGGCGCGAGCGTTTGGCCGCGAGGTGATTGCCGAGGGGCTGGAGAGTGTCGAGCACGGTCGGGTACTGATGGGCCTGGGCTGCGAGCTGGCGCAGGGTTACTGCATCGCCCGGCCGATGCCCGCCAGTGACGTGCAAGCCTGGGCACAGGCCTACCAGCAGCCAGTGCAGTGGCGTGACCCTTGAAGGTCATCAGCGGGCCGTGGTCTTGACCTGCACGGGGGGCGCCACCTG  >  NC_002947/2576270‑2576560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑