Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,577,632 G→A 9.9% Q255* (CAA→TAA)  PP_2259 ← sigma‑54 dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,577,6320GA 9.9% 106.6 / 2.1 40Q255* (CAA→TAA) PP_2259sigma‑54 dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (21/15);  new base A (2/2);  total (23/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAGGGCTGTCGAGGTTGTCGGAGGCGCTGTTGAAGATCAGTTGTGCA  >  NC_002947/2577490‑2577780
                                                                                                                                              |                                                                                                                                                    
gCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGAt                                                                                                                                                <  8:104793/149‑1 (MQ=255)
  aCTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGt                                                                                                                                              >  4:219783/1‑149 (MQ=255)
   cTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTACTCGATGtt                                                                                                                                             >  4:67698/1‑149 (MQ=255)
   cTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTACTCGATGtt                                                                                                                                             >  2:154821/1‑149 (MQ=255)
      ccGGCTTCGGGGCGGCGAGTGGTTTCGCGGGGGGGGTGCCACTGGCAGTGAAAGCGGGTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGtttt                                                                                                                                           <  4:192838/148‑1 (MQ=255)
                  cggcgAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGtt                                                                                                                              <  2:71965/149‑1 (MQ=255)
                   ggcgAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGttt                                                                                                                             <  3:219783/149‑1 (MQ=255)
                   ggcgAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGtt                                                                                                                              >  4:156009/1‑148 (MQ=255)
                    gcgAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATg                                                                                                                                               >  1:68294/1‑130 (MQ=255)
                    gcgAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATg                                                                                                                                               <  2:68294/130‑1 (MQ=255)
                                gcgTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAgg                                                                                                                 <  6:323217/148‑1 (MQ=255)
                                   tGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTAt                                                                                                             <  3:156009/149‑1 (MQ=255)
                                                 ggCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTg                                                                                               >  2:106929/1‑149 (MQ=255)
                                                    aGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTggcg                                                                                            >  7:91793/1‑149 (MQ=255)
                                                      tGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTACTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTggcggc                                                                                          <  1:154821/149‑1 (MQ=255)
                                                      tGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTACTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTggcggc                                                                                          <  3:67698/149‑1 (MQ=255)
                                                              gTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCt                                                                                  <  1:106929/149‑1 (MQ=255)
                                                                   gcCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCcg                                                                             >  8:215764/1‑149 (MQ=255)
                                                                   gcCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCcg                                                                             >  4:167631/1‑149 (MQ=255)
                                                                             cTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCg                                                                    <  4:208639/148‑1 (MQ=255)
                                                                             cTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCg                                                                    >  3:208639/1‑148 (MQ=255)
                                                                                   agCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAacac                                                               >  4:85143/1‑147 (MQ=255)
                                                                                   agCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAacac                                                               >  6:119624/1‑147 (MQ=255)
                                                                                      gAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATc                                                                     >  2:224819/1‑138 (MQ=255)
                                                                                      gAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATc                                                                     <  1:224819/138‑1 (MQ=255)
                                                                                                 gtCGGGGATGGCTGAGAAGCTGTTGCAGGGGTAACTGGAACAGTTGATCGATGTTTTGCTGCAGCGGGTTTACGACAAGCAGTATATCGGCCAGGTGGTTGTCGTCCAAAACCTGACCGCGTTCATCGAACACCAGAAAGCCGGccc                                                 >  6:325292/1‑147 (MQ=255)
                                                                                                    ggggATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAggg                                            >  2:166290/1‑149 (MQ=255)
                                                                                                     gggATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCggt                                                                                                    >  5:10408/1‑92 (MQ=255)
                                                                                                     gggATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCggt                                                                                                    <  6:10408/92‑1 (MQ=255)
                                                                                                     gggATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAGGGc                                           >  6:216151/1‑149 (MQ=255)
                                                                                                                     tGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAGGGCTGTCGAGGTTGTCg                             >  8:298125/1‑147 (MQ=255)
                                                                                                                       ttGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAGGGCTGTCGAGGTTGTCGGAg                          <  3:167631/148‑1 (MQ=255)
                                                                                                                       ttGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAGGGCTGTCGAGGTTGTCGGAg                          >  2:142252/1‑148 (MQ=255)
                                                                                                                       ttGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAGGGCTGTCGAGGTTGTCGGAg                          <  1:142252/148‑1 (MQ=255)
                                                                                                                        tGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCAGCTTTCATCGAACACCAGCAAGCCGGCCCAAGGGCTGTCGAGGTTGTCGGAg                          >  5:196767/1‑147 (MQ=255)
                                                                                                                                    tGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAGGGCTGTCGAGGTTGTCGGAGGCGCTGTTGAAGa             >  6:201752/1‑148 (MQ=255)
                                                                                                                                     ggAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAGGGCTGTCGAGGTTGTCGGAGGCGCTGTTGAAGAt            >  2:249553/1‑148 (MQ=255)
                                                                                                                                      gAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAGGGCTGTCGAGGTTGTCGGAGGCGCAGTTGAAGATCa          >  3:96254/1‑149 (MQ=255)
                                                                                                                                       aaCAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAGGGCTGTCGATGTTGTCGGAGGCGCTGTTGAAGATCa          <  5:325292/148‑1 (MQ=255)
                                                                                                                                         cAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCGGGCCCAAGGGCTGTCGAGGTTGTCGGAGGCGCTGTTGAAGATCAGtt       <  5:201752/149‑1 (MQ=255)
                                                                                                                                              gCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAGGGCTGTCGAGGTTGTCGGAGGCGCTGTTGAAGATCAGTTGTGCa  >  6:139818/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GCACTGCCGGCTTCGGGGCGGCGAGTGGTTTCGCGTGGGGGGTGCCACTGGCAGTGAAAGCGGTTGCGCCCGGTGGCCTGCAGAGCGAAGGGCTGTTGTCGGGGATGGCTGAGCAGCTGTTGCAGGGGTAACTGGAACAGTTGCTCGATGTTTTGCTGCAGCGGGTTTACGCCAAGCAGGCTATCGGCCCGGTGGTTGGCGGCCAAAACCTGCCCGCGTTCATCGAACACCAGCAAGCCGGCCCAAGGGCTGTCGAGGTTGTCGGAGGCGCTGTTGAAGATCAGTTGTGCA  >  NC_002947/2577490‑2577780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑