Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,580,273 G→A 8.3% V197I (GTC→ATC)  PP_2261 → sugar ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,580,2730GA 8.3% 133.0 / 2.5 48V197I (GTC→ATC) PP_2261sugar ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (23/21);  new base A (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

CATGGGGCGTGGCCTGGTGCGCGATGACGTGTCGGCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATACGTTCGTTGGTTATTTCATCGGTAGCCCGGGC  >  NC_002947/2580131‑2580419
                                                                                                                                              |                                                                                                                                                  
cATGGGGCGTGGCCTGGTGCGCGATGACGTGTCGGCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCAcc                                                                                                                                               <  1:74212/148‑1 (MQ=255)
      gCGTGGCCTGGTGCGCGATGACGTGTCGGCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGAcc                                                                                                                                        <  3:108400/149‑1 (MQ=255)
          ggCCTGGTGCGCGATGACGTGTCGGCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGAc                                                                                                                                         >  1:333698/1‑144 (MQ=255)
          ggCCTGGTGCGCGATGACGTGTCGGCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGAc                                                                                                                                         <  2:333698/144‑1 (MQ=255)
           gCCTGGTGCGCGATGACGTGTCGGCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGtt                                                                                                                                    <  1:259912/148‑1 (MQ=255)
                     cgATGACGTGTCGGCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGa                                                                                                                         >  4:333671/1‑149 (MQ=255)
                      gATGACGTGTCGGCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGAc                                                                                                                        <  7:172730/149‑1 (MQ=255)
                        tGACGTGTCGGCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCt                                                                                                                      >  4:92358/1‑149 (MQ=255)
                          aCGTGTCGCCGATCCTGTTAGCCGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGACGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTc                                                                                                                    <  4:24282/149‑1 (MQ=255)
                               tCGGCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGAcc                                                                                                                       >  7:254327/1‑141 (MQ=255)
                               tCGGCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGAcc                                                                                                                       <  8:254327/141‑1 (MQ=255)
                                 ggCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGAc                                                                                                              <  1:331605/148‑1 (MQ=255)
                                  gCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGAc                                                                                                              >  5:224259/1‑147 (MQ=255)
                                           ttCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGCTCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGt                                                                                                   >  4:164107/1‑149 (MQ=255)
                                           ttCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGt                                                                                                                                     <  8:37523/115‑1 (MQ=255)
                                           ttCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGt                                                                                                                                     >  7:37523/1‑115 (MQ=255)
                                                              tGATCGACCCGCATCTGAAGTGGAAGCGGCGGCGCAAGCGGAAGCAGATCCAGGAGCAGTTCAACATCCCCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATc                                                                                <  8:147930/149‑1 (MQ=255)
                                                                      ccGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCCCCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCCGCGTCCAGtt                                                                        >  4:310421/1‑149 (MQ=255)
                                                                            cTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCgg                                                                                                    >  3:97585/1‑115 (MQ=255)
                                                                            cTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCgg                                                                                                    <  4:97585/115‑1 (MQ=255)
                                                                             tGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGcaca                                                                 >  4:308180/1‑149 (MQ=255)
                                                                                    gAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACATCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACcgcgc                                                           >  3:47243/1‑148 (MQ=255)
                                                                                    gAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACATCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACcgcgc                                                           >  3:42690/1‑148 (MQ=255)
                                                                                     aaGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGa                                                         <  3:92358/149‑1 (MQ=255)
                                                                                     aaGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACATCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGa                                                         <  4:42690/149‑1 (MQ=255)
                                                                                      aGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACATCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGa                                                         <  4:47243/148‑1 (MQ=255)
                                                                                               gcAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGCGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGGTGTTCGAg                                               >  4:242539/1‑149 (MQ=255)
                                                                                                cAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGc                                              <  7:44039/149‑1 (MQ=255)
                                                                                                         gCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAg                                                        >  5:322252/1‑130 (MQ=255)
                                                                                                         gCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAg                                                        <  6:322252/130‑1 (MQ=255)
                                                                                                         gCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCgg                                      >  6:191106/1‑148 (MQ=255)
                                                                                                          cAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCa                                    >  1:183958/1‑149 (MQ=255)
                                                                                                           aGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCAt                                   <  3:308180/149‑1 (MQ=255)
                                                                                                               ccATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATAcgt                               >  5:157302/1‑149 (MQ=255)
                                                                                                                cATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATAcgtt                              <  3:333671/149‑1 (MQ=255)
                                                                                                                  tGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATAcgttcg                            >  5:43269/1‑149 (MQ=255)
                                                                                                                  tGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATAcgttcg                            >  6:321565/1‑149 (MQ=255)
                                                                                                                       aGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGTTGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATACGTTCGTTGGt                       >  7:194659/1‑149 (MQ=255)
                                                                                                                       aGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATACGTTCGTTGGt                       <  6:43269/149‑1 (MQ=255)
                                                                                                                        gTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATACGTTCGTTGGtt                      <  6:157302/149‑1 (MQ=255)
                                                                                                                            aaCATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATACGTTCGTTGGTTAttt                  >  3:58392/1‑149 (MQ=255)
                                                                                                                              cATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATACGTTCGTTGGTTATTTCa                >  3:122433/1‑149 (MQ=255)
                                                                                                                               aTCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATACGTTCGTTGGTTATTTCAt               <  4:58392/149‑1 (MQ=255)
                                                                                                                                tCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCg                                                                      <  6:154520/93‑1 (MQ=255)
                                                                                                                                tCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCg                                                                      >  5:154520/1‑93 (MQ=255)
                                                                                                                                  aCCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATACGTTCGTTGGTTATTTCATCgg            >  1:238768/1‑149 (MQ=255)
                                                                                                                                    cATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATACGTTCGTTGGTTATTTCATCgg            >  7:138698/1‑147 (MQ=255)
                                                                                                                                             cGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATACGTTCGTTGGTTATTTCATCGGTAGCCCGGGc  <  5:321565/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CATGGGGCGTGGCCTGGTGCGCGATGACGTGTCGGCGATCCTGTTCGACGAGCCGCTGACGGTGATCGACCCGCATCTGAAGTGGAAGCTGCGGCGCAAGCTGAAGCAGATCCATGAGCAGTTCAACATCACCATGATCTACGTCACCCACGACCAGTTGGAGGCCTCGACCTTCGCCGACAAGATCGCGGTGATGCACGGCGGACGCATCGTCCAGTTCGGCACACCGCGCGAGCTGTTCGAGCGACCGCGGCATACGTTCGTTGGTTATTTCATCGGTAGCCCGGGC  >  NC_002947/2580131‑2580419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑