Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,585,368 T→C 12.7% T43A (ACC→GCC)  folD‑II ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,585,3680TC12.7% 130.4 / 11.0 55T43A (ACC→GCC) folD‑IIbifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base T (26/22);  new base C (5/2);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

GTCGTCATTGAGACGGTCGATCAGCTCGGTCAGGGCTTGTTGCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATC  >  NC_002947/2585228‑2585513
                                                                                                                                            |                                                                                                                                                  
gtcgtcATTGAGACGGTCGATCAGCTCGGTCAGGGCTTGTTGCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGAc                                                                                                                                              >  8:87244/1‑147 (MQ=255)
          agaCGGTCGATCAGCTCGGTCAGGGCTTGTTGCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGc                                                                                                                                                  >  5:76129/1‑133 (MQ=255)
          agaCGGTCGATCAGCTCGGTCAGGGCTTGTTGCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGc                                                                                                                                                  <  6:76129/133‑1 (MQ=255)
             cGGTCGATCAGCTCGGTCAGGGCTTGTTGCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAg                                                                                                                               <  3:31294/149‑1 (MQ=255)
                tCGATCAGCTCGGTCAGGGCTTGTTGCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGcc                                                                                                                            >  8:107200/1‑149 (MQ=255)
                        cTCGGTCAGGGCTTGTTGCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTAc                                                                                                                    >  3:75197/1‑149 (MQ=255)
                              cAGGGCTTGTTGCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGcc                                                                                                                                 >  4:129857/1‑130 (MQ=255)
                              cAGGGCTTGTTGCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGcc                                                                                                                                 <  3:129857/130‑1 (MQ=255)
                                      gttgCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTgg                                                                                                      >  1:8412/1‑149 (MQ=255)
                                       ttgCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTgg                                                                                                      <  8:100319/148‑1 (MQ=255)
                                         gCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGa                                                                                                   >  6:255395/1‑149 (MQ=255)
                                         gCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGa                                                                                                   >  1:263374/1‑149 (MQ=255)
                                          cGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGc                                                                                                                        <  6:202378/127‑1 (MQ=255)
                                          cGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGc                                                                                                                        >  5:202378/1‑127 (MQ=255)
                                           gTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACg                                                                                                 >  7:258230/1‑149 (MQ=255)
                                              gTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTc                                                                                              >  8:4028/1‑149 (MQ=255)
                                                   gCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGa                                                                                         >  5:174914/1‑149 (MQ=255)
                                                      gggCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGacac                                                                                      >  3:167671/1‑149 (MQ=255)
                                                       ggCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGacac                                                                                      <  4:75197/148‑1 (MQ=255)
                                                         cAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGtt                                                                                   <  4:167671/149‑1 (MQ=255)
                                                            gTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCt                                                                                                        <  6:32509/125‑1 (MQ=255)
                                                            gTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCt                                                                                                        >  5:32509/1‑125 (MQ=255)
                                                                   gCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCTCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATctg                                                                         <  5:255395/149‑1 (MQ=255)
                                                                     cTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATctgct                                                                       >  8:224576/1‑149 (MQ=255)
                                                                      tGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATctgctg                                                                      >  3:129959/1‑149 (MQ=255)
                                                                        cGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTgg                                                                     <  7:4028/148‑1 (MQ=255)
                                                                         gAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGgcg                                                                   >  3:121206/1‑149 (MQ=255)
                                                                          aaaTGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGgcgc                                                                  <  6:174914/149‑1 (MQ=255)
                                                                           aaTGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCa                                                                 <  8:258230/149‑1 (MQ=255)
                                                                             tGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAgg                                                               <  7:87244/149‑1 (MQ=255)
                                                                              gAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGc                                                              >  6:346708/1‑149 (MQ=255)
                                                                                aGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTg                                                            >  4:316212/1‑149 (MQ=255)
                                                                                          ttCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAg                                                  >  6:189413/1‑149 (MQ=255)
                                                                                             gCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGcc                                                <  5:189413/148‑1 (MQ=255)
                                                                                             gCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCt                                               <  4:121206/149‑1 (MQ=255)
                                                                                                      gcgcTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGa                                     >  2:240413/1‑149 (MQ=255)
                                                                                                        gcTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGAt                                    >  1:313927/1‑148 (MQ=255)
                                                                                                               cGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGgtgt                             <  7:224576/148‑1 (MQ=255)
                                                                                                                  gaCATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGGTGTGCAg                         <  2:263374/149‑1 (MQ=255)
                                                                                                                    cATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGGTGTGCAGTc                       <  5:346708/149‑1 (MQ=255)
                                                                                                                     aTAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAg                                                                                >  5:255090/1‑92 (MQ=255)
                                                                                                                     aTAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAg                                                                                <  6:255090/92‑1 (MQ=255)
                                                                                                                       aGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGGTGTGCAGTCa                      >  3:61197/1‑147 (MQ=255)
                                                                                                                         aCTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGGTGTGCAGTCATAGa                  >  2:303033/1‑149 (MQ=255)
                                                                                                                           ttGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGGTGTGCAGTCATAGAcg                >  4:213230/1‑149 (MQ=255)
                                                                                                                              ggAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGGTGTGCAGTCATAGACGCGt             <  3:213230/149‑1 (MQ=255)
                                                                                                                                 atcctggTCGGCACCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGGTGTGCAGTCATAGACGCGTGAt          >  5:108148/6‑149 (MQ=255)
                                                                                                                                   cctggTCGGCACCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCAACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGGTGTGCAGTCATAGACGCGTGAtt         >  4:326252/4‑148 (MQ=255)
                                                                                                                                     tggTCGGCACCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAgg                                >  3:160010/2‑123 (MQ=255)
                                                                                                                                     tggTCGGCACCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAgg                                <  4:160010/122‑1 (MQ=255)
                                                                                                                                     tggTCGGCACCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGgtgt                             >  2:243305/2‑126 (MQ=255)
                                                                                                                                     tggTCGGCACCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGgtgt                             <  1:243305/125‑1 (MQ=255)
                                                                                                                                     tggTCGGCACCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAAc      >  4:124843/2‑149 (MQ=255)
                                                                                                                                         cGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATc  <  4:129959/149‑1 (MQ=255)
                                                                                                                                          ggTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTGCCCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATc  <  1:240413/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
GTCGTCATTGAGACGGTCGATCAGCTCGGTCAGGGCTTGTTGCGTGGTCTCGCTGGGCAGGTCGAAAGCCTGCGAAATGAAGCCGACCTCTTCGCAGTCCTTGCGCTTGTGCGAGACATAGACTTGGGAGGCGGGGTCGGTGCCGACCAGGATCACTGCCAGGCCCGGCGTACGCAGGCCTTGCTGGCGACGCTCCACGACACGTTGAGCGATCTGCTGGCGCAGGCTGGCGGCGATAGCCTTG‑CCATCGATTAGGTGTGCAGTCATAGACGCGTGATTAACCATC  >  NC_002947/2585228‑2585513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑