Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,619,640 G→A 7.8% W60* (TGG→TGA)  PP_2293 → DNA maturase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,619,6400GA 7.8% 137.6 / 2.4 51W60* (TGG→TGA) PP_2293DNA maturase B
Reads supporting (aligned to +/- strand):  ref base G (25/22);  new base A (2/2);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

GAAGACTTTCGAAAGTTTATCTACCTGGTATGGAAACACCTCAACCTCCCCGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCAAACGCCTCATCCACGAAATGCCGTTGCTCCAGCACCTCAA  >  NC_002947/2619494‑2619786
                                                                                                                                                  |                                                                                                                                                  
gAAGACTTTCGAAAGTTTATCTACCTGGTATGGAAACACCTCAACCTCCCCGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGt                                                                                                                                                  >  5:302957/1‑149 (MQ=255)
      tttCGAAAGTTTATCTACCTGGTATGGAAACACCTCAACCTCCCCGAACCCACCCCCGTCCAATTCGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGCCAAGTCATGGGTCACAGc                                                                                                                                            >  4:117262/1‑149 (MQ=255)
         cGAAAGTTTATCTACCTGGTATGGAAACACCTCAACCTCCCCGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCg                                                                                                                                          >  2:268135/1‑148 (MQ=255)
               tttATCTACCTGGTATGGAAACACCTCAACCTCCCCGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCgt                                                                                                                                   <  5:177818/149‑1 (MQ=255)
                    cTACCTGGTATGGAAACACCTCAACCTCCCCGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCt                                                                                                                              >  1:168077/1‑149 (MQ=255)
                       ccTGGTATGGAAACACCTCAACCTCCCCGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGc                                                                                                                               <  3:232031/145‑1 (MQ=255)
                         tGGTATGGAAACACCTCAACCTCCCCGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATgg                                                                                                                                                    <  5:65534/122‑1 (MQ=255)
                         tGGTATGGAAACACCTCAACCTCCCCGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTACCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATgg                                                                                                                                                    >  6:65534/1‑122 (MQ=255)
                                gAAACACCTCAACCTCCCCGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGc                                                                                                                  <  7:16379/149‑1 (MQ=255)
                                          aaCCTCCCCGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTg                                                                                                                    >  4:292642/1‑137 (MQ=255)
                                          aaCCTCCCCGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTg                                                                                                                    <  3:292642/137‑1 (MQ=255)
                                                cccGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCg                                                                                                  >  5:115039/1‑149 (MQ=255)
                                                  cGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCa                                                                                                >  5:185946/1‑149 (MQ=255)
                                                        cacccCAGTCCAATACGACATCGACCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTgg                                                                                          <  8:80465/149‑1 (MQ=255)
                                                         acccCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTgg                                                                                          >  7:142135/1‑148 (MQ=255)
                                                         acccCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCAATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGt                                                                                         <  8:157849/149‑1 (MQ=255)
                                                          cccccGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTa                                                                                        <  8:142135/149‑1 (MQ=255)
                                                             ccGACCAAAACGACATCCCCCGATACCTGCAACACGGTCCCAGGCGATGCGTCATTGAAGCCTTCCGAGGAGTCGGCAAGTCATCGCTCACACACGCATTCGTGTGCTGGCTCCTATGGCGAAATCCACAACTCCGCATCCTGGTAgtg                                                                                     >  4:93549/1‑149 (MQ=255)
                                                                                ccGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGctc                                                                                                                         <  8:1623/94‑1 (MQ=255)
                                                                                ccGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGctc                                                                                                                         >  7:1623/1‑94 (MQ=255)
                                                                                      ccTGCAACACGGTCCCCGGCGATGCGTCATTGATGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGAt                                                             <  8:261696/148‑1 (MQ=255)
                                                                                      ccTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGAt                                                             <  6:302957/148‑1 (MQ=255)
                                                                                       cTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGc                                                           >  2:106757/1‑149 (MQ=255)
                                                                                            acacGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCCATGCGTtct                                                      >  2:338948/1‑149 (MQ=255)
                                                                                             cacGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAATTCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTtctc                                                     <  1:268135/149‑1 (MQ=255)
                                                                                             cacGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTtctc                                                     <  6:185946/149‑1 (MQ=255)
                                                                                                ggTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGAGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAg                                                  >  2:11610/1‑149 (MQ=255)
                                                                                                 gTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACATCCCCAGGCGGCTGCTTGATCCGCTGCGGAAATCCCACAGACCGGCTGCGGGTAGGGACAGACTCCAGCGCCCGCTGCGAATCGTGGTCCAGc                                                 >  6:166508/1‑149 (MQ=255)
                                                                                                  tCCCCGGCGATGCGTCATTGAAGGCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCATCTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGGCGATGCGTTCTCCAGCt                                                <  3:93549/149‑1 (MQ=255)
                                                                                                    cccGGCGATGAGACATTGAAGCCTTCAGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATACGTTTGATGGCTCCACTGGCGAAATCCCCAACTCCGCATCATGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTATCCAGCtgc                                              >  4:295990/1‑147 (MQ=255)
                                                                                                     ccGGCGATTCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTCTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCAGCATCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCg                                             >  6:137481/1‑149 (MQ=255)
                                                                                                     ccGGCGATGGTTGATTGAAGCCTTCCGAGGAGTGGTCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCACTTGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCGCGCGCCGATGCGTTCTCCAGCTTCg                                             <  3:295990/149‑1 (MQ=255)
                                                                                                     ccGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTa                                                                                        <  1:162400/106‑1 (MQ=255)
                                                                                                     ccGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTa                                                                                        >  2:162400/1‑106 (MQ=255)
                                                                                                       ggCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTACGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTc                                           <  6:115039/149‑1 (MQ=255)
                                                                                                          gATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGAGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCaaa                                        <  1:11610/149‑1 (MQ=255)
                                                                                                               gTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTAAACCGCACGCGCCCATCAGTTCTCCAGCTACGTCAAACGCCt                                   >  4:225509/1‑149 (MQ=255)
                                                                                                                   ttGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGc                                                                                                                  <  6:164904/66‑1 (MQ=255)
                                                                                                                    tGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCAAACGCCTCATc                               >  1:23966/1‑148 (MQ=255)
                                                                                                                      aaGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCAAACGCCTCATCCa                             <  1:106757/148‑1 (MQ=255)
                                                                                                                           ttCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTCCTCCAGCTTCGTCAAACGACTCATCCACGAAAt                       >  7:304737/1‑149 (MQ=255)
                                                                                                                                  gagTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCAAACGCCTCATCCACGAAATGCCGTTg                >  4:257928/1‑149 (MQ=255)
                                                                                                                                  gagTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCAAACGCCTCATCCACGAAATGCCGTTg                >  4:253516/1‑149 (MQ=255)
                                                                                                                                  gagTGGGCAAGTCATGAGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCAAACGCCTCATCCACGAAATg                      <  1:55444/143‑1 (MQ=255)
                                                                                                                                  gagTGGGCAAGTCATGAGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCAAACGCCTCATCCACGAAATg                      >  2:55444/1‑143 (MQ=255)
                                                                                                                                      gggCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCAAACGCCTCATCCACGAAATGCCGTTGCTc             >  5:134807/1‑148 (MQ=255)
                                                                                                                                       ggCAAGTCATGGGTCGCAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAgc                                                                         <  1:215732/87‑1 (MQ=255)
                                                                                                                                       ggCAAGTCATGGGTCGCAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAgc                                                                         >  2:215732/1‑87 (MQ=255)
                                                                                                                                       ggCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGGCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCAAACGCCTCATCCACGAAATGCCGTTGCTCCa           <  3:117262/149‑1 (MQ=255)
                                                                                                                                        gCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTg                                                                                           >  4:330768/1‑68 (MQ=255)
                                                                                                                                        gCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTg                                                                                           <  3:330768/68‑1 (MQ=255)
                                                                                                                                             tCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCAAACGCCTCATCCACGAAATGCCGTTGCTCCAGCACCt     >  3:26391/1‑149 (MQ=255)
                                                                                                                                                tGGGGCACAGCCGCATTCGTGTCCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCAAACGCCTCATCCACGAAATGCCGTTGCTCCAGCACCTCaa  <  7:151629/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GAAGACTTTCGAAAGTTTATCTACCTGGTATGGAAACACCTCAACCTCCCCGAACCCACCCCCGTCCAATACGACATCGCCCGATACCTGCAACACGGTCCCCGGCGATGCGTCATTGAAGCCTTCCGAGGAGTGGGCAAGTCATGGGTCACAGCCGCATTCGTGTGCTGGCTCCTCTGGCGAAATCCCCAACTCCGCATCCTGGTAGTGTCCGCCTCCAGCGCCCGCGCCGATGCGTTCTCCAGCTTCGTCAAACGCCTCATCCACGAAATGCCGTTGCTCCAGCACCTCAA  >  NC_002947/2619494‑2619786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑