Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,690,895 A→C 7.6% T79P (ACG→CCG)  PP_2357 → type I pili subunit CsuB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,690,8950AC 7.6% 138.5 / 2.1 50T79P (ACG→CCG) PP_2357type I pili subunit CsuB
Reads supporting (aligned to +/- strand):  ref base A (25/21);  new base C (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGTTCGGCCTGTTGTTCAGCGGCAGTACCGTGGCGGCGGATTTTCTCGTGGAAGTGCGGGTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCGAACTGCGCTACCTG  >  NC_002947/2690750‑2691041
                                                                                                                                                 |                                                                                                                                                  
gatGTTCGTCCTGTTGTTCAGCGTCATTAACGTGGCGGCGGATTTTCTCGTGGAAGTGCGGGTACTCGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGAGCTGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGCCgg                                                                                                                                                 >  6:117957/3‑149 (MQ=255)
         ccTGTTGTTCAGCGGCAGTACCGTGGCGGCGGATTTTCTCGTGGAAGTGCGGGTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGCGCGCCGTCGCCCCtag                                                                                                                                        <  8:114959/149‑3 (MQ=255)
         ccTGTTGGGCCGCGGCAGTACCGGGCCGGCGGACTTCCTCCTGCACGCGCGGGGACTGGTCCAGCGTGGCTGCATGCTGCTCACCCACCCCCGCGATGCGGGAGCTCAGGCCCCGGGACGTATCGACCTCGCGGCGACGGCGCGCCTgg                                                                                                                                        <  6:268894/149‑1 (MQ=255)
          cTGTTGTTCAGCGGCAGTACCGTGGCGGCGGATTTTCTCGTGGAAGTGCGGGTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGa                                                                                                                                       <  6:185720/149‑1 (MQ=255)
            gttgttCAGCGGCAGTACCGTGGCGGCGGATTTTCTCGTGGAAGTGCGGGTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATg                                                                                                                                     >  5:76771/1‑149 (MQ=255)
               gttCAGCGGCAGTACCGTGGCGGCGGATTTTCTCGTGGAAGTGCGGGTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGc                                                                                                                                   <  5:85287/148‑1 (MQ=255)
               gttCAGCGGCAGTACCGTGGCGGCGGATTTTCTCGTGGAAGTGCGGGTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCGAGGGGCGACGGCGCGCCTGGAAGGcc                                                                                                                                  >  7:154248/1‑149 (MQ=255)
                 tCAGCGGCAGTACCGTGGCGGCGGATTTTCTCGTGGAAGTGCGGGTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCt                                                                                                                                >  7:134632/1‑149 (MQ=255)
                   aGCGGCAGTACCGTGGCGGCGGATTTTCTCGTGGAAGTGCGGGTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTgg                                                                                                                              >  7:280330/1‑149 (MQ=255)
                    gCGGCAGTACCGTGGCGGCGGATTTTCTCGTGGAAGTGCGGGTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGgc                                                                                                                             >  3:291217/1‑149 (MQ=255)
                                                 gtgtAAGTGCGGGTACTGGTGCAGCGTGGCTGCAGGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCGTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCa                                                                                                <  5:117957/149‑1 (MQ=255)
                                                    gAAGTGCGGGTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGc                                                                                              <  8:280330/148‑1 (MQ=255)
                                                          cGGGTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTccgc                                                                                        <  7:264690/148‑1 (MQ=255)
                                                             gTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGTGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCcgcg                                                                                    >  1:305433/1‑149 (MQ=255)
                                                             gTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGAGATGCGGGAGCTAAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTCGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCTCcgcg                                                                                    >  5:102957/1‑149 (MQ=255)
                                                                  ggTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGGCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTg                                                                                <  8:154248/148‑1 (MQ=255)
                                                                      cAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTg                                                                           <  6:76771/149‑1 (MQ=255)
                                                                        gCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGc                                                                          >  3:26501/1‑148 (MQ=255)
                                                                        gCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGCGCGCCGTCGCCCCtag                                                                                                                                        >  6:235061/1‑84 (MQ=255)
                                                                        gCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGCGCGCCGTCGCCCCtag                                                                                                                                        <  5:235061/86‑3 (MQ=255)
                                                                                     cTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGcc                                                            >  8:22892/1‑149 (MQ=255)
                                                                                        gTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTa                                                         >  5:89725/1‑149 (MQ=255)
                                                                                           aagcAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAgt                                                                                                                 <  7:73506/87‑1 (MQ=255)
                                                                                           aaCCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCTGCGCCTGGAGTGCAACCCGGATACGCCCTATCa                                                      <  4:291217/149‑1 (MQ=255)
                                                                                            aCCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGc                                                             <  3:196902/141‑1 (MQ=255)
                                                                                            aCCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGc                                                             >  4:196902/1‑141 (MQ=255)
                                                                                            aCCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAg                                                     >  8:224050/1‑149 (MQ=255)
                                                                                             ccAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAgg                                                    >  1:14384/1‑149 (MQ=255)
                                                                                             ccAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCGTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAgg                                                    >  5:83555/1‑149 (MQ=255)
                                                                                                gACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGGCCTTGAGTGGTGTGCTGTTGAGCGAGCGTCCGCCGCGCCTGGATTTCAAGCCGGATACGGTCTATCACGTgc                                                 <  2:179690/149‑1 (MQ=255)
                                                                                                gACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTgc                                                 <  5:13112/149‑1 (MQ=255)
                                                                                                   ccGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGCCCTAGGGGCGACGGCGCGCCTGGATGGCCCTGCCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGcgcg                                              >  7:231128/1‑149 (MQ=255)
                                                                                                   ccGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGcgcg                                              >  5:90215/1‑149 (MQ=255)
                                                                                                   ccGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGcgcg                                              >  1:318713/1‑149 (MQ=255)
                                                                                                   ccGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGcgcg                                              >  1:50388/1‑149 (MQ=255)
                                                                                                            gggAGCTCAGGCCCTGGGACGTATCGACCTCGTGGCGACGGCGCGCCTGGATGTCCCTGGCGCGCCCTTGAGTGGTGTGCGGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGAtggtg                                     <  3:203631/149‑1 (MQ=255)
                                                                                                                        ccTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCAtggtg                         <  2:14384/149‑1 (MQ=255)
                                                                                                                               cGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCgg                  <  7:224050/149‑1 (MQ=255)
                                                                                                                                 tATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCg                <  6:89725/149‑1 (MQ=255)
                                                                                                                                 tATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCg                <  8:231128/149‑1 (MQ=255)
                                                                                                                                  aTCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCg                >  5:107321/1‑148 (MQ=255)
                                                                                                                                   tCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCGaa              >  6:254134/1‑149 (MQ=255)
                                                                                                                                        cTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCGAACTgcg         <  6:102957/149‑1 (MQ=255)
                                                                                                                                          aGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCGAACTgcgc        >  1:225527/1‑148 (MQ=255)
                                                                                                                                          aGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCGAACTgcgc        >  6:299607/1‑148 (MQ=255)
                                                                                                                                            gggCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCGAACTGCGCTAc     <  4:26501/149‑1 (MQ=255)
                                                                                                                                            gggCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCGAACTGCGCTAc     >  6:269357/1‑149 (MQ=255)
                                                                                                                                              gCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCGAACTGCGCTACCt   >  3:148352/1‑149 (MQ=255)
                                                                                                                                               cGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCGAACTGCGCTACCTg  <  8:134632/149‑1 (MQ=255)
                                                                                                                                               cGACGGCGCGCCTGAATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCGAACTGCGCTACCTg  >  4:8516/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCTGTTCGGCCTGTTGTTCAGCGGCAGTACCGTGGCGGCGGATTTTCTCGTGGAAGTGCGGGTACTGGTGCAGCGTGGCTGCATGCTGGTCAACCAGACCCGCGATGCGGGAGCTCAGGCCCTGGGACGTATCGACCTAGGGGCGACGGCGCGCCTGGATGGCCCTGGCGCGCCCTTGAGTGGTGTGCTGTTGAGCCAGCGTCCGCCGCGCCTGGAGTGCAACCCGGATACGCCCTATCAGGTGCGCGTCGATGGTGGCCAGCATGGTGGCATCGGCGAACTGCGCTACCTG  >  NC_002947/2690750‑2691041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑