Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,723,641 T→G 7.1% S88R (AGC→CGC)  PP_2384 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,723,6410TG 7.1% 171.1 / 4.2 56S88R (AGC→CGC) PP_2384membrane protein
Reads supporting (aligned to +/- strand):  ref base T (26/26);  new base G (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GCGAAGCGCTGCTGATAGTAGCGAGCGTGCAGCATCTCGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAGGCATGAAGATGATCGGGCCGCAGATGGCGGTGAGCATGCCCGGCACGGCAAA  >  NC_002947/2723494‑2723788
                                                                                                                                                   |                                                                                                                                                    
gCGAAGCGCTGCTGATAGTAGCGAGCGTGCAGCATCTCGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTc                                                                                                                                                     <  3:222099/149‑1 (MQ=255)
  gAAGCGCTGCTGATAGTAGCGAGCGTGCAGCATCTCGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAg                                                                                                                                                   >  6:232909/1‑149 (MQ=255)
        ctgctgATAGTAGCGAGCGTGCAGCATCTCGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATAc                                                                                                                                             >  2:277293/1‑149 (MQ=255)
               tagtagCGAGCGTGCAGCATCTCGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCgg                                                                                                                                      <  1:277293/149‑1 (MQ=255)
                  tagCGAGCGTGCAGCATCTCGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGt                                                                                                                                    >  6:189510/1‑148 (MQ=255)
                     cgagcgTGCAGCATCTCGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAAc                                                                                                                                <  5:114213/149‑1 (MQ=255)
                       agcgTGCAGCATCTCGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAg                                                                                                                              <  5:232909/149‑1 (MQ=255)
                             cagcaTCTCGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAg                                                                                                                        >  4:60244/1‑149 (MQ=255)
                                   ctcGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCaa                                                                                                                                 <  4:152629/134‑1 (MQ=255)
                                   ctcGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCaa                                                                                                                                 >  3:152629/1‑134 (MQ=255)
                                     cGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATgg                                                                                                                >  7:162851/1‑149 (MQ=255)
                                            gCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAg                                                                                                         >  2:211912/1‑149 (MQ=255)
                                                  ggCTGGCGCCTGGCAGAGGTGTGTGTGCTCGCGGAAGTGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCTACATGCTCACCAGCATTCTCATCAATTCGCTGCGGGTCAACTCCACCAGGTCGATGGCTACAAG‑GGAGcc                                                                                                  <  6:274590/149‑1 (MQ=255)
                                                  ggCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCg                                                                                                                                       <  5:6903/113‑1 (MQ=255)
                                                  ggCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCg                                                                                                                                       >  6:6903/1‑113 (MQ=255)
                                                           cTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAgg                                                                                           >  8:45652/1‑147 (MQ=255)
                                                                agagGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGt                                                                                    >  2:45675/1‑149 (MQ=255)
                                                                 gagGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGtt                                                                                   <  5:189510/149‑1 (MQ=255)
                                                                 gagGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGtt                                                                                   <  7:45652/149‑1 (MQ=255)
                                                                    gtgtgtCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAg‑g                                                                                                       >  8:78171/1‑126 (MQ=255)
                                                                    gtgtgtCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAg‑g                                                                                                       <  7:78171/126‑1 (MQ=255)
                                                                     tgtgtCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAggg                                                                                      <  8:151127/142‑1 (MQ=255)
                                                                     tgtgtCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAggg                                                                                      >  7:151127/1‑142 (MQ=255)
                                                                       tgtCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTGACCAGGATGCTCAGCAATACGCTGCGGGTCAACAGGACCAGGGCGATGGCTACAAGCGGAGCCCAGCAGGTAGGGGTTGAGCa                                                                              <  1:4330/149‑1 (MQ=255)
                                                                       tgtCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTTCAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAg                                                                             <  1:211912/149‑1 (MQ=255)
                                                                       tgtCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCGCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTAc                                                                                                            >  7:173956/1‑119 (MQ=255)
                                                                       tgtCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCGCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTAc                                                                                                            >  7:169034/1‑119 (MQ=255)
                                                                       tgtCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCGCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTAc                                                                                                            <  8:169034/119‑1 (MQ=255)
                                                                       tgtCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCGCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTAc                                                                                                            <  8:173956/119‑1 (MQ=255)
                                                                        gtCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAgg                                                                            >  2:57147/1‑149 (MQ=255)
                                                                         tCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAgg                                                                            <  2:307364/148‑1 (MQ=255)
                                                                                gcgcAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAgg                                                                      <  8:162851/147‑1 (MQ=255)
                                                                                gcgcAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGtt                                                                    >  2:5332/1‑149 (MQ=255)
                                                                                   cAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAg‑g                                                                                                       <  5:316178/111‑1 (MQ=255)
                                                                                   cAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAg‑g                                                                                                       >  6:316178/1‑111 (MQ=255)
                                                                                    aaGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGc                                                                >  1:182841/1‑149 (MQ=255)
                                                                                     aGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCt                                                               >  5:94959/1‑149 (MQ=255)
                                                                                      gggcgggcTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCtg                                                              <  3:60244/149‑1 (MQ=255)
                                                                                         cgggcTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCtggtg                                                           <  6:94959/149‑1 (MQ=255)
                                                                                              tCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAg                                                      >  5:203030/1‑149 (MQ=255)
                                                                                              tCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAg                                                      >  8:24902/1‑149 (MQ=255)
                                                                                                aTGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAg                                                      <  1:5332/147‑1 (MQ=255)
                                                                                                  gccagccaGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTCGTCAGGCAt                                                  >  8:56412/1‑149 (MQ=255)
                                                                                                      gccagcTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAGGCATGAAg                                              <  6:203030/149‑1 (MQ=255)
                                                                                                       ccagcTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCGGGTGGTCAGGCATg                                                 >  1:31274/1‑145 (MQ=255)
                                                                                                                    aggaagAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑CGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAGGCATGAAGATGATCGGGCCg                                  >  8:165559/1‑147 (MQ=255)
                                                                                                                      gaagaaGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAGGCATGAAGATGATCGGGCCg                                  >  6:36588/1‑145 (MQ=255)
                                                                                                                            gaTCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAGGCATGAAGATGATCGGGCCg                                  >  4:185278/1‑139 (MQ=255)
                                                                                                                            gaTCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAGGCATGAAGATGATCGGGCCg                                  <  3:185278/139‑1 (MQ=255)
                                                                                                                              tCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAGGCATGAAGATGATCGGGCCGCAGATGGCGGt                       <  2:31274/148‑1 (MQ=255)
                                                                                                                                aaCATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCACAGGATGAGCTGGTGGTCATGAATGAAGATGATAGGGCAGCAGATGGAGGTGAg                    >  8:221233/1‑149 (MQ=255)
                                                                                                                                     gCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAg                                                                             >  2:171975/1‑87 (MQ=255)
                                                                                                                                     gCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAg                                                                             <  1:171975/87‑1 (MQ=255)
                                                                                                                                            aGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAGGCATGAAGATGATCGGGCCGCAGATGGCGGTGAGCATGCCcggcac        <  5:36588/149‑1 (MQ=255)
                                                                                                                                               aTGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAGGCATGAAGATGATCGGGCCGCAGATGGCGGTGAGCATGCCcggcacggc     <  2:182841/149‑1 (MQ=255)
                                                                                                                                                  cTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCATCACGTAGGGGTTTATCAGGCTCAGGTTGATCTGGTGGTCAGGCATGAAGATGATCGGGCCGCAGATGGCGGTGAGCATGCCCGGCACGGCaaa  <  7:221233/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGAAGCGCTGCTGATAGTAGCGAGCGTGCAGCATCTCGACGTCGCCAAGGGCTGGCGCCTGCCAGAGGTGTGTCTGCTCGCGCAAGGGCGGGCTCATGCCAGCCAGCTGCGCAGAAGGAAGAAGATCAACATGCTCACCAGCATGCTCAGCAATACGCTGCGGGTCAACAGCACCAGGGCGATGGCTACAAG‑GGAGCCCAGCAGGTAGGGGTTGAGCAGGCTCAGGTTGAGCTGGTGGTCAGGCATGAAGATGATCGGGCCGCAGATGGCGGTGAGCATGCCCGGCACGGCAAA  >  NC_002947/2723494‑2723788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑