| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,745,175 | G→T | 11.4% | intergenic (+18/+17) | PP_2399 → / ← PP_2400 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,745,175 | 0 | G | T | 11.4% | 93.2 / 4.6 | 36 | intergenic (+18/+17) | PP_2399/PP_2400 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (24/6); new base T (2/2); total (26/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
ACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGT > NC_002947/2745056‑2745317 | aCCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATAGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCTGCGCAGcccccaaat > 4:205305/1‑131 (MQ=255)aCCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTCTCATTGTCGCAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCTGCGCAGccccccaat < 3:205305/135‑5 (MQ=255) cGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCg > 2:275146/1‑149 (MQ=255) gggCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAAAGATCGAGGc > 7:207418/1‑148 (MQ=255) ggCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAAAAGTGTGAAGGCATTTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGc < 1:275146/148‑1 (MQ=255) gggTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAggcgc < 4:305206/137‑1 (MQ=255) gggTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAggcgc > 3:305206/1‑137 (MQ=255) ttGCTGGAAGCTTTTGGCGCGCAGGGTGTCTTTGTGGGAACAGGGTGACGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGt < 6:196809/149‑1 (MQ=255) tGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGgca > 5:89318/1‑149 (MQ=255) ggAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTCCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCATGAGCAGCAGTGgcag > 2:291040/1‑149 (MQ=255) gAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGgcag > 1:262150/1‑148 (MQ=255) aTTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGc > 3:25521/1‑149 (MQ=255) tttCGCGAGCAGGGTGGCATTGACGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGcc < 7:253145/149‑1 (MQ=255) tCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGccac > 1:322749/1‑149 (MQ=255) ctgggTGTCATTGGCGTAACATTGTTACGGCT‑TTGGGGGG‑CGGCCCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAg < 2:262150/147‑1 (MQ=255) atggTCGGACCAGTGTGATGG‑A‑TTGGGGGGTCGCCCCACCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGc < 4:182217/146‑1 (MQ=255) aTTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCgg > 7:211229/1‑148 (MQ=255) ttGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGc > 2:38078/1‑148 (MQ=255) tCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACt > 3:190611/1‑149 (MQ=255) tCGGAACAGTGTGAAGGCA‑TAGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACt > 2:205417/1‑149 (MQ=255) gAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGt > 2:170228/1‑149 (MQ=255) cAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCAGAAAAGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCAACCAGGTAGATCAGCGCCAACTTGTAAt > 3:22371/1‑149 (MQ=255) gtgtGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTg > 6:243787/1‑149 (MQ=255) tgtgAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGc > 8:44722/1‑149 (MQ=255) tgtgAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGc > 4:8455/1‑149 (MQ=255) tgtgAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAAAGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGc < 8:207418/149‑1 (MQ=255) tgAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGcc > 5:43025/1‑148 (MQ=255) tgAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGcc > 1:255674/1‑148 (MQ=255) gAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCg > 5:158480/1‑148 (MQ=255) aaGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCg > 7:298856/1‑147 (MQ=255) gCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGcc > 5:225852/1‑149 (MQ=255) a‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCTGAAGCCCt > 5:47393/1‑149 (MQ=255) ggggg‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTc < 8:298856/149‑1 (MQ=255) ggg‑CTGCTGCGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGTAGCAACGCCACCAGGTAGATCAGc > 6:166133/1‑118 (MQ=255) ggg‑CTGCTGCGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGTAGCAACGCCACCAGGTAGATCAGc < 5:166133/118‑1 (MQ=255) g‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGt > 3:319184/1‑148 (MQ=255) | ACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGT > NC_002947/2745056‑2745317 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |