Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,745,177 A→C 11.0% intergenic (+20/+15) PP_2399 → / ← PP_2400 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,745,1770AC11.0% 93.4 / 3.7 36intergenic (+20/+15)PP_2399/PP_2400hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/8);  new base C (2/2);  total (26/10)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

ACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCA  >  NC_002947/2745056‑2745324
                                                                                                                           |                                                                                                                                                   
aCCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATAGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCTGCGCAGcccccaaat                                                                                                                                        >  4:205305/1‑131 (MQ=255)
aCCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTCTCATTGTCGCAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCTGCGCAGccccccaat                                                                                                                                        <  3:205305/135‑5 (MQ=255)
  cGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCg                                                                                                                        >  2:275146/1‑149 (MQ=255)
                          gggCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAAAGATCGAGGc                                                                                                 >  7:207418/1‑148 (MQ=255)
                           ggCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAAAAGTGTGAAGGCATTTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGc                                                                                                 <  1:275146/148‑1 (MQ=255)
                                             gggTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAggcgc                                                                                         <  4:305206/137‑1 (MQ=255)
                                             gggTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAggcgc                                                                                         >  3:305206/1‑137 (MQ=255)
                                                      ttGCTGGAAGCTTTTGGCGCGCAGGGTGTCTTTGTGGGAACAGGGTGACGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGt                                                                    <  6:196809/149‑1 (MQ=255)
                                                          tGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGgca                                                                >  5:89318/1‑149 (MQ=255)
                                                           ggAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTCCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCATGAGCAGCAGTGgcag                                                               >  2:291040/1‑149 (MQ=255)
                                                            gAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGgcag                                                               >  1:262150/1‑148 (MQ=255)
                                                                 aTTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGc                                                         >  3:25521/1‑149 (MQ=255)
                                                                  tttCGCGAGCAGGGTGGCATTGACGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGcc                                                        <  7:253145/149‑1 (MQ=255)
                                                                    tCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGccac                                                      >  1:322749/1‑149 (MQ=255)
                                                                           ctgggTGTCATTGGCGTAACATTGTTACGGCT‑TTGGGGGG‑CGGCCCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAg                                               <  2:262150/147‑1 (MQ=255)
                                                                                    atggTCGGACCAGTGTGATGG‑A‑TTGGGGGGTCGCCCCACCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGc                                      <  4:182217/146‑1 (MQ=255)
                                                                                    aTTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCgg                                       >  7:211229/1‑148 (MQ=255)
                                                                                     ttGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGc                                      >  2:38078/1‑148 (MQ=255)
                                                                                        tCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACt                                  >  3:190611/1‑149 (MQ=255)
                                                                                        tCGGAACAGTGTGAAGGCA‑TAGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACt                                  >  2:205417/1‑149 (MQ=255)
                                                                                           gAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGt                               >  2:170228/1‑149 (MQ=255)
                                                                                              cAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCAGAAAAGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCAACCAGGTAGATCAGCGCCAACTTGTAAt                            >  3:22371/1‑149 (MQ=255)
                                                                                                gtgtGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTg                          >  6:243787/1‑149 (MQ=255)
                                                                                                 tgtgAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGc                         >  8:44722/1‑149 (MQ=255)
                                                                                                 tgtgAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGc                         >  4:8455/1‑149 (MQ=255)
                                                                                                 tgtgAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAAAGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGc                         <  8:207418/149‑1 (MQ=255)
                                                                                                   tgAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGcc                        >  5:43025/1‑148 (MQ=255)
                                                                                                   tgAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGcc                        >  1:255674/1‑148 (MQ=255)
                                                                                                    gAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCg                       >  5:158480/1‑148 (MQ=255)
                                                                                                     aaGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCg                       >  7:298856/1‑147 (MQ=255)
                                                                                                        gCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGcc                  >  5:225852/1‑149 (MQ=255)
                                                                                                          a‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCTGAAGCCCt                >  5:47393/1‑149 (MQ=255)
                                                                                                               ggggg‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTc            <  8:298856/149‑1 (MQ=255)
                                                                                                                 ggg‑CTGCTGCGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGTAGCAACGCCACCAGGTAGATCAGc                                         >  6:166133/1‑118 (MQ=255)
                                                                                                                 ggg‑CTGCTGCGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGTAGCAACGCCACCAGGTAGATCAGc                                         <  5:166133/118‑1 (MQ=255)
                                                                                                                   g‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGt         >  3:319184/1‑148 (MQ=255)
                                                                                                                          cagcagCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCa  >  6:154837/1‑149 (MQ=255)
                                                                                                                          cagcagCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCa  <  6:225852/149‑1 (MQ=255)
                                                                                                                           |                                                                                                                                                   
ACCGGAAGAGAGCGGCTGCAGGCATTGGGCGGGGATGTGCAGCAAGGGTTGGCATTGCTGGAAGCATTTCGCGAGCAGGGTGTCATTGTCGGAACAGTGTGAAGGCA‑TTGGGGGG‑CTGCGCAGCAGCCCCCGATCCTCATCTGCCGAACCGCATGCTCAGCCAATGATCGAGGCTAAGGCGCCCGGCGCCCTTGAGCAGCAGTGGCAGCAACGCCACCAGGTAGATCAGCGGCAACTTGTAATTGCCGAAGCCCTGGTCGGTAATGGCA  >  NC_002947/2745056‑2745324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑