Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,747,971 G→A 7.7% P179S (CCG→TCG)  PP_2403 ← chemotaxis protein CheY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,747,9710GA 7.7% 146.1 / 2.2 52P179S (CCG→TCG) PP_2403chemotaxis protein CheY
Reads supporting (aligned to +/- strand):  ref base G (29/19);  new base A (2/2);  total (31/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

CGGTCTTGATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGG  >  NC_002947/2747824‑2748111
                                                                                                                                                     |                                                                                                                                            
cGGTCTTGATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCggg                                                                                                                                             <  2:323218/149‑1 (MQ=255)
        aTGCAGCTGGGCTCGCGGGTATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAg                                                                                                                                     <  8:236271/149‑1 (MQ=255)
        aTGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGa                                                                                                                                      <  7:14477/148‑1 (MQ=255)
            aGCTGGGCTCACGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGaa                                                                                                                                  >  6:159640/1‑148 (MQ=255)
                    tCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCgcag                                                                                                                         >  1:337365/1‑149 (MQ=255)
                        gggCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCTGCTGCGAGTGTTCCTCGACCACCCGCAGCggg                                                                                                                     <  7:306906/149‑1 (MQ=38)
                         ggCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGc                                                                                                                    >  1:21915/1‑149 (MQ=255)
                           cATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCgg                                                                                                                  <  1:173281/149‑1 (MQ=255)
                            aTCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGt                                                                                                                 <  2:337365/149‑1 (MQ=255)
                                    ccAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTc                                                                                                                             >  8:5282/1‑129 (MQ=255)
                                    ccAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTc                                                                                                                             <  7:5282/129‑1 (MQ=255)
                                          gcTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCTGCTGCGAGTGTTCCTCGACCACCCGCAGCgggtgc                                                                                                                  <  6:287250/134‑4 (MQ=37)
                                          gcTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCTGCTGCGAGTGTTCCTCGACCACCCGCAGCgggtgc                                                                                                                  >  5:287250/1‑131 (MQ=37)
                                            tGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAg                                                                                                 >  5:219164/1‑149 (MQ=255)
                                                gcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACCCTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGc                                                                                              >  5:186675/1‑148 (MQ=255)
                                                gcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGcc                                                                                             >  5:190487/1‑149 (MQ=255)
                                                gcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGc                                                                                              >  5:181861/1‑148 (MQ=255)
                                                gcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGc                                                                                              >  1:274787/1‑148 (MQ=255)
                                                   ggcggcTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGGGTCAGGTTAGGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACAATCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGcca                                                                                            >  1:267750/1‑149 (MQ=255)
                                                       gcTGACCAGCAAGGCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGGCAGGGTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAggg                                                                                      <  1:96149/149‑1 (MQ=255)
                                                            ccAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCt                                                                                 <  6:280243/149‑1 (MQ=255)
                                                            ccAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCt                                                                                 >  6:320450/1‑149 (MQ=255)
                                                               gCAAGTCGATGGAGCGGTCGAAGATGTCGGCTGCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCGGCTCAGGGCCACCAGGGTGCCTTCg                                                                              <  4:48400/149‑1 (MQ=255)
                                                                  aGTCGATGGACCGGGCGAAGATGTGGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCTCTCAGGGCCACCAGGGTGCCTTCGCTg                                                                           <  3:20100/149‑1 (MQ=255)
                                                                       aTGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAggg                                                                                               <  2:340645/124‑1 (MQ=255)
                                                                       aTGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAggg                                                                                               >  1:340645/1‑124 (MQ=255)
                                                                         ggAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCtt                                                                                <  8:166919/137‑1 (MQ=255)
                                                                         ggAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCtt                                                                                >  7:166919/1‑137 (MQ=255)
                                                                           aGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCgc                                                                                                                           <  4:128136/92‑1 (MQ=255)
                                                                           aGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCgc                                                                                                                           >  3:128136/1‑92 (MQ=255)
                                                                                    aGATGTCGGCTTCGCGGCCCT‑‑GGGCCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGcgcgcg                                                         >  4:122862/1‑149 (MQ=255)
                                                                                       tGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtg                                                      <  2:274787/149‑1 (MQ=255)
                                                                                       tGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtg                                                      <  5:320450/149‑1 (MQ=255)
                                                                                       tGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtg                                                      <  6:190487/149‑1 (MQ=255)
                                                                                       tGTCGGCTTCGCGGCCCT‑‑GGGCCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtg                                                      <  3:122862/149‑1 (MQ=255)
                                                                                                 gcgGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGgc                                            >  2:254555/1‑149 (MQ=255)
                                                                                                        t‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGTTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGgcgc                                          >  2:30777/1‑144 (MQ=255)
                                                                                                        t‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGTTGCCTTCGCTGTCCAGCAGGTGGCGCGCGGTGGTGTCCAGgcgc                                          <  1:30777/144‑1 (MQ=255)
                                                                                                        t‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCAtt                                      >  7:233470/1‑148 (MQ=255)
                                                                                                              tCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGAcc                                  >  4:58205/1‑148 (MQ=255)
                                                                                                                  gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAc                             >  7:40687/1‑149 (MQ=255)
                                                                                                                  gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAc                             >  4:313244/1‑149 (MQ=255)
                                                                                                                  gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAc                             >  2:264239/1‑149 (MQ=255)
                                                                                                                   ttAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAcc                            >  5:11471/1‑149 (MQ=255)
                                                                                                                       gcagcTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCcag                         >  1:142278/1‑148 (MQ=255)
                                                                                                                              ttCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAggcggc                 >  6:69258/1‑149 (MQ=255)
                                                                                                                                    ctcgaccACCCGCAGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCttt           <  1:225236/143‑1 (MQ=255)
                                                                                                                                    ctcgaccACCCGCAGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCttt           >  2:225236/7‑149 (MQ=255)
                                                                                                                                     cTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTc          <  5:69258/149‑1 (MQ=255)
                                                                                                                                       gAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCAc        >  1:37314/1‑149 (MQ=255)
                                                                                                                                         gCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACt       >  1:184127/1‑148 (MQ=255)
                                                                                                                                            cctgCTGCGAGTGTTCCTCGACCACCCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATg   >  8:138194/4‑149 (MQ=255)
                                                                                                                                             ccGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATgg  >  8:108522/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
CGGTCTTGATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGG  >  NC_002947/2747824‑2748111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑