Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,747,975 G→T 7.7% D177E (GAC→GAA)  PP_2403 ← chemotaxis protein CheY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,747,9750GT 7.7% 161.3 / 4.0 52D177E (GAC→GAA) PP_2403chemotaxis protein CheY
Reads supporting (aligned to +/- strand):  ref base G (29/19);  new base T (2/2);  total (31/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

ATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGG  >  NC_002947/2747832‑2748111
                                                                                                                                                 |                                                                                                                                        
aTGCAGCTGGGCTCGCGGGTATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAg                                                                                                                                     <  8:236271/149‑1 (MQ=255)
aTGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGa                                                                                                                                      <  7:14477/148‑1 (MQ=255)
    aGCTGGGCTCACGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGaa                                                                                                                                  >  6:159640/1‑148 (MQ=255)
            tCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCgcag                                                                                                                         >  1:337365/1‑149 (MQ=255)
                gggCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCTGCTGCGAGTGTTCCTCGACCACCCGCAGCggg                                                                                                                     <  7:306906/149‑1 (MQ=38)
                 ggCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGc                                                                                                                    >  1:21915/1‑149 (MQ=255)
                   cATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCgg                                                                                                                  <  1:173281/149‑1 (MQ=255)
                    aTCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGt                                                                                                                 <  2:337365/149‑1 (MQ=255)
                            ccAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTc                                                                                                                             >  8:5282/1‑129 (MQ=255)
                            ccAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTc                                                                                                                             <  7:5282/129‑1 (MQ=255)
                                  gcTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCTGCTGCGAGTGTTCCTCGACCACCCGCAGCgggtgc                                                                                                                  <  6:287250/134‑4 (MQ=37)
                                  gcTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCTGCTGCGAGTGTTCCTCGACCACCCGCAGCgggtgc                                                                                                                  >  5:287250/1‑131 (MQ=37)
                                    tGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAg                                                                                                 >  5:219164/1‑149 (MQ=255)
                                        gcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACCCTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGc                                                                                              >  5:186675/1‑148 (MQ=255)
                                        gcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGcc                                                                                             >  5:190487/1‑149 (MQ=255)
                                        gcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGc                                                                                              >  1:274787/1‑148 (MQ=255)
                                        gcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGc                                                                                              >  5:181861/1‑148 (MQ=255)
                                           ggcggcTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGGGTCAGGTTAGGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACAATCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGcca                                                                                            >  1:267750/1‑149 (MQ=255)
                                               gcTGACCAGCAAGGCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGGCAGGGTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAggg                                                                                      <  1:96149/149‑1 (MQ=255)
                                                    ccAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCt                                                                                 <  6:280243/149‑1 (MQ=255)
                                                    ccAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCt                                                                                 >  6:320450/1‑149 (MQ=255)
                                                       gCAAGTCGATGGAGCGGTCGAAGATGTCGGCTGCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCGGCTCAGGGCCACCAGGGTGCCTTCg                                                                              <  4:48400/149‑1 (MQ=255)
                                                          aGTCGATGGACCGGGCGAAGATGTGGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCTCTCAGGGCCACCAGGGTGCCTTCGCTg                                                                           <  3:20100/149‑1 (MQ=255)
                                                               aTGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAggg                                                                                               >  1:340645/1‑124 (MQ=255)
                                                               aTGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAggg                                                                                               <  2:340645/124‑1 (MQ=255)
                                                                 ggAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCtt                                                                                <  8:166919/137‑1 (MQ=255)
                                                                 ggAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCtt                                                                                >  7:166919/1‑137 (MQ=255)
                                                                   aGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCgc                                                                                                                           >  3:128136/1‑92 (MQ=255)
                                                                   aGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCgc                                                                                                                           <  4:128136/92‑1 (MQ=255)
                                                                            aGATGTCGGCTTCGCGGCCCT‑‑GGGCCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGcgcgcg                                                         >  4:122862/1‑149 (MQ=255)
                                                                               tGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtg                                                      <  2:274787/149‑1 (MQ=255)
                                                                               tGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtg                                                      <  5:320450/149‑1 (MQ=255)
                                                                               tGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtg                                                      <  6:190487/149‑1 (MQ=255)
                                                                               tGTCGGCTTCGCGGCCCT‑‑GGGCCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtg                                                      <  3:122862/149‑1 (MQ=255)
                                                                                         gcgGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGgc                                            >  2:254555/1‑149 (MQ=255)
                                                                                                t‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGTTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGgcgc                                          >  2:30777/1‑144 (MQ=255)
                                                                                                t‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGTTGCCTTCGCTGTCCAGCAGGTGGCGCGCGGTGGTGTCCAGgcgc                                          <  1:30777/144‑1 (MQ=255)
                                                                                                t‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCAtt                                      >  7:233470/1‑148 (MQ=255)
                                                                                                      tCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGAcc                                  >  4:58205/1‑148 (MQ=255)
                                                                                                          gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAc                             >  2:264239/1‑149 (MQ=255)
                                                                                                          gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAc                             >  7:40687/1‑149 (MQ=255)
                                                                                                          gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAc                             >  4:313244/1‑149 (MQ=255)
                                                                                                           ttAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAcc                            >  5:11471/1‑149 (MQ=255)
                                                                                                               gcagcTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCcag                         >  1:142278/1‑148 (MQ=255)
                                                                                                                      ttCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAggcggc                 >  6:69258/1‑149 (MQ=255)
                                                                                                                            ctcgaccACCCGCAGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCttt           >  2:225236/7‑149 (MQ=255)
                                                                                                                            ctcgaccACCCGCAGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCttt           <  1:225236/143‑1 (MQ=255)
                                                                                                                             cTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTc          <  5:69258/149‑1 (MQ=255)
                                                                                                                               gAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCAc        >  1:37314/1‑149 (MQ=255)
                                                                                                                                 gCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACt       >  1:184127/1‑148 (MQ=255)
                                                                                                                                    cctgCTGCGAGTGTTCCTCGACCACCCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATg   >  8:138194/4‑149 (MQ=255)
                                                                                                                                     ccGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATgg  >  8:108522/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                        
ATGCAGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGG  >  NC_002947/2747832‑2748111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑