Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,747,983 A→C 7.6% F175V (TTC→GTC)  PP_2403 ← chemotaxis protein CheY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,747,9830AC 7.6% 158.1 / 4.3 53F175V (TTC→GTC) PP_2403chemotaxis protein CheY
Reads supporting (aligned to +/- strand):  ref base A (30/19);  new base C (2/2);  total (32/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

AGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAGGTTGGGCGGC  >  NC_002947/2747836‑2748125
                                                                                                                                                     |                                                                                                                                              
aGCTGGGCTCACGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGaa                                                                                                                                                >  6:159640/1‑148 (MQ=255)
        tCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCgcag                                                                                                                                       >  1:337365/1‑149 (MQ=255)
            gggCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCTGCTGCGAGTGTTCCTCGACCACCCGCAGCggg                                                                                                                                   <  7:306906/149‑1 (MQ=38)
             ggCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGc                                                                                                                                  >  1:21915/1‑149 (MQ=255)
               cATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCgg                                                                                                                                <  1:173281/149‑1 (MQ=255)
                aTCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGt                                                                                                                               <  2:337365/149‑1 (MQ=255)
                        ccAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTc                                                                                                                                           >  8:5282/1‑129 (MQ=255)
                        ccAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTc                                                                                                                                           <  7:5282/129‑1 (MQ=255)
                              gcTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCTGCTGCGAGTGTTCCTCGACCACCCGCAGCgggtgc                                                                                                                                <  6:287250/134‑4 (MQ=37)
                              gcTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCTGCTGCGAGTGTTCCTCGACCACCCGCAGCgggtgc                                                                                                                                >  5:287250/1‑131 (MQ=37)
                                tGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAg                                                                                                               >  5:219164/1‑149 (MQ=255)
                                    gcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACCCTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGc                                                                                                            >  5:186675/1‑148 (MQ=255)
                                    gcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGcc                                                                                                           >  5:190487/1‑149 (MQ=255)
                                    gcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGc                                                                                                            >  1:274787/1‑148 (MQ=255)
                                    gcAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGc                                                                                                            >  5:181861/1‑148 (MQ=255)
                                       ggcggcTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCTGGGGGTCAGGTTAGGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACAATCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGcca                                                                                                          >  1:267750/1‑149 (MQ=255)
                                           gcTGACCAGCAAGGCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGGCAGGGTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAggg                                                                                                    <  1:96149/149‑1 (MQ=255)
                                                ccAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCt                                                                                               >  6:320450/1‑149 (MQ=255)
                                                ccAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCt                                                                                               <  6:280243/149‑1 (MQ=255)
                                                   gCAAGTCGATGGAGCGGTCGAAGATGTCGGCTGCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCGGCTCAGGGCCACCAGGGTGCCTTCg                                                                                            <  4:48400/149‑1 (MQ=255)
                                                      aGTCGATGGACCGGGCGAAGATGTGGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCTCTCAGGGCCACCAGGGTGCCTTCGCTg                                                                                         <  3:20100/149‑1 (MQ=255)
                                                           aTGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAggg                                                                                                             >  1:340645/1‑124 (MQ=255)
                                                           aTGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAggg                                                                                                             <  2:340645/124‑1 (MQ=255)
                                                             ggAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCtt                                                                                              <  8:166919/137‑1 (MQ=255)
                                                             ggAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCtt                                                                                              >  7:166919/1‑137 (MQ=255)
                                                               aGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCgc                                                                                                                                         <  4:128136/92‑1 (MQ=255)
                                                               aGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCgc                                                                                                                                         >  3:128136/1‑92 (MQ=255)
                                                                        aGATGTCGGCTTCGCGGCCCT‑‑GGGCCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGcgcgcg                                                                       >  4:122862/1‑149 (MQ=255)
                                                                           tGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtg                                                                    <  6:190487/149‑1 (MQ=255)
                                                                           tGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtg                                                                    <  5:320450/149‑1 (MQ=255)
                                                                           tGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtg                                                                    <  2:274787/149‑1 (MQ=255)
                                                                           tGTCGGCTTCGCGGCCCT‑‑GGGCCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCggtg                                                                    <  3:122862/149‑1 (MQ=255)
                                                                                     gcgGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGgc                                                          >  2:254555/1‑149 (MQ=255)
                                                                                            t‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGTTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGgcgc                                                        >  2:30777/1‑144 (MQ=255)
                                                                                            t‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGTTGCCTTCGCTGTCCAGCAGGTGGCGCGCGGTGGTGTCCAGgcgc                                                        <  1:30777/144‑1 (MQ=255)
                                                                                            t‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCAtt                                                    >  7:233470/1‑148 (MQ=255)
                                                                                                  tCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGAcc                                                >  4:58205/1‑148 (MQ=255)
                                                                                                      gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAc                                           >  4:313244/1‑149 (MQ=255)
                                                                                                      gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAc                                           >  2:264239/1‑149 (MQ=255)
                                                                                                      gTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAc                                           >  7:40687/1‑149 (MQ=255)
                                                                                                       ttAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAAcc                                          >  5:11471/1‑149 (MQ=255)
                                                                                                           gcagcTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCcag                                       >  1:142278/1‑148 (MQ=255)
                                                                                                                  ttCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAggcggc                               >  6:69258/1‑149 (MQ=255)
                                                                                                                        ctcgaccACCCGCAGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCttt                         >  2:225236/7‑149 (MQ=255)
                                                                                                                        ctcgaccACCCGCAGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCttt                         <  1:225236/143‑1 (MQ=255)
                                                                                                                         cTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTc                        <  5:69258/149‑1 (MQ=255)
                                                                                                                           gAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCAc                      >  1:37314/1‑149 (MQ=255)
                                                                                                                             gCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACt                     >  1:184127/1‑148 (MQ=255)
                                                                                                                                cctgCTGCGAGTGTTCCTCGACCACCCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATg                 >  8:138194/4‑149 (MQ=255)
                                                                                                                                 ccGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATgg                >  8:108522/1‑149 (MQ=255)
                                                                                                                                              tCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAGGTTGggcgg   >  6:41491/1‑149 (MQ=255)
                                                                                                                                                gAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAGGTTGggcggc  <  2:37314/148‑1 (MQ=255)
                                                                                                                                                 aGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAg            >  6:265401/1‑137 (MQ=255)
                                                                                                                                                 aGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAg            <  5:265401/137‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
AGCTGGGCTCGCGGGCATCGTCACCCAGGCGCTGGCGCAGGCGGCTGACCAGCAAGTCGATGGAGCGGTCGAAGATGTCGGCTTCGCGGCCCT‑‑GGGTCAGGTTAAGCAGCTGTTCGCGGCTGAGCACCCGCTGCGGGTGGTCGAGGAACACTCGCAGCAGGCGGTACTCGGCGCCGCTCAGGGCCACCAGGGTGCCTTCGCTGTCGAGCAGGTGGCGCGCGGTGGTGTCCAGGCGCCATTGACCGAAACCCAGCAGGCGGCTGCTTTCACTGATGGTCAGGTTGGGCGGC  >  NC_002947/2747836‑2748125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑