Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,752,898	A→C	5.6%	Q152P (CAG→CCG)	czcB‑II →	cobalt‑zinc‑cadmium resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,752,898	0	A	C	5.6%	229.4 / 4.3	71	Q152P (CAG→CCG)	czcB‑II	cobalt‑zinc‑cadmium resistance protein
Reads supporting (aligned to +/- strand):  ref base A (37/30);  new base C (2/2);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

ACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGCCGAGATCGCCCTGGCCAATGCTC  >  NC_002947/2752754‑2753041                                                                                                                                                 |                                                                                                                                                 aCGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGcc                                                                                                                                              >  6:183403/1‑149 (MQ=255) aCGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGcc                                                                                                                                              >  2:316061/1‑149 (MQ=255)        ccgcaccgcaCATGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAAc                                                                                                                                       <  7:73608/149‑1 (MQ=255)             ccgcacATGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGc                                                                                                                                  >  6:13551/1‑149 (MQ=255)                     gtggtgCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAgg                                                                                                                                                <  4:209452/127‑1 (MQ=255)                     gtggtgCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAgg                                                                                                                                                >  3:209452/1‑127 (MQ=255)                           ccccGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAACAGGCCGCTCAACGTCGCCTGG‑AACTGGcgcg                                                                                                                   >  7:245181/1‑149 (MQ=255)                                   gCCGGGTGTGGTCGAAGCGGTGCAAGCAGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCt                                                                                                           >  5:131763/1‑149 (MQ=255)                                           tgGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAACAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACgg                                                                                                   <  8:245181/149‑1 (MQ=255)                                             gTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGa                                                                                                 <  1:316061/149‑1 (MQ=255)                                              tCGAAGCGGTGCAAGCCGAGCTGGGCCAGGTGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGaa                                                                                                <  3:44910/149‑1 (MQ=255)                                              tCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACg                                                                                                    >  1:228200/1‑145 (MQ=255)                                              tCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACg                                                                                                    <  2:228200/145‑1 (MQ=255)                                               cGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGAc                                                                                                             <  6:241596/135‑1 (MQ=255)                                               cGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGAc                                                                                                             >  5:241596/1‑135 (MQ=255)                                                gAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTc                                                                                                                                    >  6:191896/1‑112 (MQ=255)                                                gAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTc                                                                                                                                    <  5:191896/112‑1 (MQ=255)                                                  aGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGaacaa                                                                                             >  4:134558/1‑148 (MQ=255)                                                      gTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCt                                                                                        >  2:195952/1‑149 (MQ=255)                                                      gTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCt                                                                                        >  2:112744/1‑149 (MQ=255)                                                      gTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCACTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCt                                                                                        >  6:314057/1‑149 (MQ=255)                                                        gCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCttt                                                                                                         <  8:260713/130‑1 (MQ=255)                                                        gCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCttt                                                                                                         >  7:260713/1‑130 (MQ=255)                                                             ccGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAg                                                                                 >  7:30707/1‑149 (MQ=255)                                                              cGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAg                                                                                 >  8:106648/1‑148 (MQ=255)                                                                 gCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGc                                                                             <  3:134558/149‑1 (MQ=255)                                                                     ggCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGaa                                                                          <  5:139967/148‑1 (MQ=255)                                                                     ggCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAAt                                                                         >  4:20595/1‑149 (MQ=255)                                                                      gCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCaa                                                                                                      >  7:294466/1‑119 (MQ=255)                                                                      gCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCaa                                                                                                      <  8:294466/119‑1 (MQ=255)                                                                       ccAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCa                                                                       <  5:13551/149‑1 (MQ=255)                                                                       ccAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCa                                                                       <  1:246980/149‑1 (MQ=255)                                                                         aGGCGGTCAAGCGCGGCCAGGTACTGGCTGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCTCGGTTGACCTTTCAACGTGAACAACAGCTGTGGc                                                                                   <  3:295327/135‑1 (MQ=255)                                                                         aGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGAATGCCAGCCAGCAGATATCCGACCTGCGCACTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACAGGCGCGGTTGACATTTCAACGGGAACAACAGCTGTGGc                                                                                   >  4:295327/1‑135 (MQ=255)                                                                           gCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAgcgc                                                                   >  2:130581/1‑149 (MQ=255)                                                                             ggTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGcc                                                                  >  1:182614/1‑148 (MQ=255)                                                                              gTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCACTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGa                                                                <  5:314057/149‑1 (MQ=255)                                                                                        gCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTAc                                                      <  4:96069/149‑1 (MQ=255)                                                                                         ccAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTAcc                                                     >  4:233822/1‑149 (MQ=255)                                                                                             gTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCCGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTg                                                   >  5:28110/1‑147 (MQ=255)                                                                                              tACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAg                                                <  1:112744/149‑1 (MQ=255)                                                                                              tACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAg                                                <  3:20595/149‑1 (MQ=255)                                                                                                 tGGCGGTGATTGCCAGCCTGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGc                                                                             <  5:133053/117‑1 (MQ=255)                                                                                                 tGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGc                                                                             >  6:133053/1‑117 (MQ=255)                                                                                                   gCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCAc                                            <  5:183403/148‑1 (MQ=255)                                                                                                     ggTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTc                                         <  6:131763/149‑1 (MQ=255)                                                                                                         aTTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAATGGGAACAACAGCAGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGgc                                     >  5:62376/1‑149 (MQ=255)                                                                                                             ccagccagCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGGAAAATGGCGCGGTTGACATTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATAAGCGCCGAGAAGGATTACCTGCAGGCACGTCAGGCGCt                                  >  1:271234/1‑149 (MQ=255)                                                                                                             ccagccagCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTg                                 <  2:182614/149‑1 (MQ=255)                                                                                                               agccagcAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTg                                                                                                                               <  4:329882/54‑1 (MQ=255)                                                                                                               agccagcAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTg                                                                                                                               >  3:329882/1‑54 (MQ=255)                                                                                                                gccagcAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAg                                                <  7:199468/131‑1 (MQ=255)                                                                                                                gcaagcagATCTACGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTAGCCTGG‑AACTGGCGAGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGAGAATCAGCGCCGAGCAGTAGTACCTGCAg                                                >  8:199468/4‑131 (MQ=255)                                                                                                                    gcagATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGc                                                                             <  8:183134/98‑1 (MQ=255)                                                                                                                      agATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGcc                        >  7:7171/1‑149 (MQ=255)                                                                                                                      agATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGcc                        >  4:235270/1‑149 (MQ=255)                                                                                                                         tctcCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGcc                        >  4:26653/1‑146 (MQ=255)                                                                                                                           tcCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGtt                                                                                                                >  7:135857/1‑56 (MQ=255)                                                                                                                           tcCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGtt                                                                                                                <  8:135857/56‑1 (MQ=255)                                                                                                                            ccGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGcc                        >  6:156027/1‑143 (MQ=255)                                                                                                                             cGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGCCgag                     <  1:130581/145‑1 (MQ=255)                                                                                                                                ccTGCGCAGTGAGCAGCCGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGCCGAGATCGccc              <  6:28110/149‑1 (MQ=255)                                                                                                                                ccTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGCCGAGATCGccc              >  2:205536/1‑149 (MQ=255)                                                                                                                                 cTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGcc                        >  5:305454/1‑138 (MQ=255)                                                                                                                                 cTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGcc                        <  6:305454/138‑1 (MQ=255)                                                                                                                                   gcgcAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGCCGAGATCGCCCTgg           >  6:205128/1‑149 (MQ=255)                                                                                                                                    cgcAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGCCGAGATCGCCCTGGc          <  2:271234/149‑1 (MQ=255)                                                                                                                                      cAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGCCGAGATCGCCCTGGcc         >  5:130172/1‑148 (MQ=255)                                                                                                                                       gttGAGCGGCCTGCTGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGCCGAGATCg                 <  3:286816/137‑1 (MQ=255)                                                                                                                                       gttGAGCGGCCTGCTGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGCCGAGATCg                 >  4:286816/3‑139 (MQ=255)                                                                                                                                         tGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGCCGAGATCGCCCTGGCCAATg     >  2:295799/1‑149 (MQ=255)                                                                                                                                            gcagcaGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGCCGAGATCGCCCTGGCCAATGCTc  <  3:235270/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 ACGAGGACCGCACCGCACATGTGGTGCCCCGCGTGCCGGGTGTGGTCGAAGCGGTGCAAGCCGAGCTGGGCCAGGCGGTCAAGCGCGGCCAGGTACTGGCGGTGATTGCCAGCCAGCAGATCTCCGACCTGCGCAGTGAGCAGCAGGCCGCTCAACGTCGCCTGG‑AACTGGCGCGGTTGACCTTTCAACGGGAACAACAGCTGTGGCAGGAGCGAATCAGCGCCGAGCAGGATTACCTGCAGGCACGTCAGGCGCTGCAGGAGGCCGAGATCGCCCTGGCCAATGCTC  >  NC_002947/2752754‑2753041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑