Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,753,888 G→T 6.6% A72S (GCG→TCG)  czcA‑II → cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,753,8880GT 6.6% 234.5 / 3.8 74A72S (GCG→TCG) czcA‑IIcation efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (36/33);  new base T (3/2);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

ATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAGCAATT  >  NC_002947/2753741‑2754036
                                                                                                                                                   |                                                                                                                                                    
aTGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACcg                                                                                                                                                      <  2:58469/148‑1 (MQ=255)
      gcgGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGAtggc                                                                                                                                               <  5:66300/149‑1 (MQ=255)
        ggTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGAtggctg                                                                                                                                             <  4:164313/149‑1 (MQ=255)
          tGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGCGGTCTCGAtggcaaag                                                                                                                                           <  4:102794/149‑5 (MQ=37)
            ggTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCt                                                                                                                                         >  4:37932/1‑149 (MQ=255)
                  cacaGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAgg                                                                                                                                   >  3:3057/1‑149 (MQ=255)
                   acaGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAggg                                                                                                                                  <  8:285641/149‑1 (MQ=255)
                          ccAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGCGGTCTCGAtggcaaagg                                                                                                                                          <  1:341216/134‑6 (MQ=38)
                          ccAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGCGGTCTCGAtggcaaagg                                                                                                                                          >  2:341216/1‑129 (MQ=38)
                          ccAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAg                                                                                                                            <  4:3057/148‑1 (MQ=255)
                           cAGAAACTGCCGATCGATGCTGTGCTGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAg                                                                                                                            >  6:291746/1‑147 (MQ=255)
                           cAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAg                                                                                                                            >  3:153471/1‑147 (MQ=255)
                            aGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAg                                                                                                                            >  8:85713/1‑146 (MQ=255)
                            aGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAg                                                                                                                            >  4:176324/1‑146 (MQ=255)
                            aGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAg                                                                                                                            >  7:194324/1‑146 (MQ=255)
                              aaaCTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGAc                                                                                                                       <  3:155159/149‑1 (MQ=255)
                                   gCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCcgct                                                                                                                  >  2:252512/1‑149 (MQ=255)
                                    ccGATCGATGCTGTGCTGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCcgctc                                                                                                                 <  5:291746/149‑1 (MQ=255)
                                    ccGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCcgctc                                                                                                                 <  5:231396/149‑1 (MQ=255)
                                     cgatcgatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCcgctcg                                                                                                                <  1:252512/149‑1 (MQ=255)
                                      gatcgatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCcgctcgc                                                                                                               >  1:343176/1‑149 (MQ=255)
                                          gatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTc                                                                                                           <  8:194324/149‑1 (MQ=255)
                                          gatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTc                                                                                                           <  3:176324/149‑1 (MQ=255)
                                            tGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTcgc                                                                                                         >  6:307978/1‑149 (MQ=255)
                                                  gCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCgg                                                                                                   >  5:121701/1‑149 (MQ=255)
                                                    cGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGa                                                                                                                        >  2:31549/1‑126 (MQ=255)
                                                    cGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGa                                                                                                                        <  1:31549/126‑1 (MQ=255)
                                                      gACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCt                                                                                               >  6:226218/1‑149 (MQ=255)
                                                             cTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTAATCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCACACCCGCTCGCGCTCGCGCTCGGACCTGTCGc                                                                                          >  2:168981/1‑147 (MQ=39)
                                                              tAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAg                                                                                       >  5:179266/1‑149 (MQ=255)
                                                              tAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGATCGGGCCTTTCGCAAg                                                                                       >  6:266512/1‑149 (MQ=255)
                                                                          gCAGATCAACACCGCCGCCCCCGGATATTCGCCGCTCGAGACCGAGCAGCGCATCCCCTTTGCCATCGAGACCGCGAACGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTAGCCCTCGGGCCTGTCGCGAGTCACGGTCAgtt                                                                           >  8:246957/1‑149 (MQ=255)
                                                                           cAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCAttt                                                                           >  2:239111/1‑148 (MQ=255)
                                                                             gATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTc                                                                                            >  5:42376/1‑129 (MQ=255)
                                                                             gATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTc                                                                                            <  6:42376/129‑1 (MQ=255)
                                                                                 aaCACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGc                                                                                                 <  7:310515/120‑1 (MQ=255)
                                                                                 aaCACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGc                                                                                                 >  8:310515/1‑120 (MQ=255)
                                                                                 aaCACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatga                                                                    <  1:239111/149‑1 (MQ=255)
                                                                                 aaCACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatga                                                                    <  7:85713/149‑1 (MQ=255)
                                                                                  acacCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGGAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGACCTGTCGCAAGTCACGGTCATTTTCgatgat                                                                   <  1:168981/149‑1 (MQ=255)
                                                                                  acacCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatgat                                                                   <  6:179266/149‑1 (MQ=255)
                                                                                   ctccgccgCCCCCCGCCACTCGCCGCGGGCGACCGAGCAGCGCATCACCCTTGCCCTCGAGCCCGCGCTGGCTGGCCCGCCAGGGCTCAAGCAGACCCGCCCGCGGCCGCGCTCGGGCCTGTCGCAAGCCACGGGCACTTTCGatgatg                                                                  <  4:218581/147‑1 (MQ=255)
                                                                                   cacCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGatgatg                                                                  >  8:624/1‑149 (MQ=255)
                                                                                        ccgccCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTc                                                                         <  1:190434/137‑1 (MQ=255)
                                                                                        ccgccCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTc                                                                         >  2:190434/1‑137 (MQ=255)
                                                                                        ccgccCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGatgatg                                                                  >  3:218581/1‑144 (MQ=255)
                                                                                             cccGGCTATTCGCCGCTCGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGatat                                                        >  6:257939/1‑149 (MQ=255)
                                                                                              ccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa                                                                                        >  1:20368/1‑116 (MQ=255)
                                                                                              ccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa                                                                                        <  2:20368/116‑1 (MQ=255)
                                                                                                ggCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATAtctt                                                     <  2:343176/149‑1 (MQ=255)
                                                                                                 gCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATAtcttc                                                    <  7:101408/149‑1 (MQ=255)
                                                                                                 gCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATAtcttc                                                    >  5:109482/1‑149 (MQ=255)
                                                                                                     ttCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATAtcttct                                                   >  6:207672/1‑146 (MQ=255)
                                                                                                     ttCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATAtcttct                                                   <  5:207672/146‑1 (MQ=255)
                                                                                                        gccgcTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTcgccc                                             <  5:226218/149‑1 (MQ=255)
                                                                                                        gccgcTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTcgccc                                             >  4:304549/1‑149 (MQ=255)
                                                                                                                  aCCGATCAGCGAATGACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGTGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGt                                   <  5:266512/149‑1 (MQ=255)
                                                                                                                  aCCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGt                                                                                >  1:196597/1‑104 (MQ=255)
                                                                                                                  aCCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGt                                                                                <  2:196597/104‑1 (MQ=255)
                                                                                                                    cGAGCAGCGCATCACCTTTGCCATCGAGACTGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTc                                  >  3:268294/1‑148 (MQ=255)
                                                                                                                    cGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCCCCGATATCTTCTTCGCCCGCCAACTGGTc                                  >  5:238578/1‑148 (MQ=255)
                                                                                                                    cGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCa                                 <  7:624/149‑1 (MQ=255)
                                                                                                                      agcagcGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAAc                               <  3:37932/149‑1 (MQ=255)
                                                                                                                              aTCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCt                       <  3:304549/149‑1 (MQ=255)
                                                                                                                              aTCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCt                       >  3:12534/1‑149 (MQ=255)
                                                                                                                               tCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTg                      >  7:57055/1‑149 (MQ=255)
                                                                                                                                  ccTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAg                   >  6:305290/1‑149 (MQ=255)
                                                                                                                                   gccagCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAgg                  >  7:145370/5‑149 (MQ=255)
                                                                                                                                   gccagCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAgg                  >  6:22215/5‑149 (MQ=255)
                                                                                                                                          aTCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCcg           >  2:184698/1‑149 (MQ=255)
                                                                                                                                            cGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTcgcccgc                                           <  5:207221/115‑1 (MQ=255)
                                                                                                                                            cGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTcgcccgc                                           >  6:207221/1‑115 (MQ=255)
                                                                                                                                             gagaCCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCGGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGACAACTGGTCAACGAGCGCCTGCAGGGGGCCCGCGa        <  7:246957/149‑1 (MQ=255)
                                                                                                                                              agaCCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAg       <  8:57055/149‑1 (MQ=255)
                                                                                                                                              acaCCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAg       <  4:203707/147‑1 (MQ=255)
                                                                                                                                                   gcgATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTTGTCAACGAGCGCCTGCAGGTGGCCCGCGAGCAAtt  <  5:257939/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGATGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAGCAATT  >  NC_002947/2753741‑2754036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑