Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,799,171 A→G 11.7% intergenic (‑185/+31) PP_2452 ← / ← ansB hypothetical protein/glutaminase‑asparaginase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,799,1710AG11.7% 126.7 / 7.8 51intergenic (‑185/+31)PP_2452/ansBhypothetical protein/glutaminase‑asparaginase
Reads supporting (aligned to +/- strand):  ref base A (22/23);  new base G (2/4);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.62e-01

ACTGTAAGTAAATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCG  >  NC_002947/2799028‑2799316
                                                                                                                                               |                                                                                                                                                 
aCTGTAAGTAAATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCgg                                                                                                                                              >  2:109678/1‑149 (MQ=255)
 cTGTAATTAAATTCGCTACTGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGTCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGgc                                                                                                                                             <  4:86837/149‑1 (MQ=255)
   gtaagtaaATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGgcg                                                                                                                                            >  3:122160/1‑148 (MQ=255)
    taagtaaATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCAGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCa                                                                                                                                          >  1:243277/1‑149 (MQ=255)
        taaATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCgg                                                                                                                                      >  5:340292/1‑149 (MQ=255)
            ttCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCt                                                                                                                                  >  5:14636/1‑149 (MQ=255)
               gCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGACGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAg                                                                                                                               >  2:122955/1‑149 (MQ=255)
                 aaCAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGGCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGAggg                                                                                                                             <  2:105165/149‑1 (MQ=255)
                  actgCTTGTCAGGTGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAATGGTCAACGATGTCGCCGGTTTGTAACCTCTGTGGCTACGCGGTCGAGGAATTGCCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGAgggc                                                                                                                            <  1:229374/146‑2 (MQ=255)
                     gCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGaa                                                                                                                         <  2:243277/149‑1 (MQ=255)
                     gCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGaa                                                                                                                         <  7:266532/149‑1 (MQ=255)
                     gCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGaa                                                                                                                         <  6:340292/149‑1 (MQ=255)
                      cTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGa                                                                                                                                <  8:24617/141‑1 (MQ=255)
                      cTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGa                                                                                                                                >  7:24617/1‑141 (MQ=255)
                      cTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAg                                                                                                                        >  4:128158/1‑149 (MQ=255)
                       ttGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAg                                                                                                                        >  5:105737/1‑148 (MQ=255)
                        tGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCa                                                                                                                      >  7:54033/1‑149 (MQ=255)
                          tCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGACGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACt                                                                                                                    <  1:122955/149‑1 (MQ=255)
                            aGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAAGCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCa                                                                                                                  <  1:109678/149‑1 (MQ=255)
                            aGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCa                                                                                                                  <  1:105315/149‑1 (MQ=255)
                                      cAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCag                                                                                                        <  8:54033/149‑1 (MQ=255)
                                      cAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCa                                                                                                         <  6:14636/148‑1 (MQ=255)
                                             ggCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATc                                                                                                  <  4:122160/148‑1 (MQ=255)
                                                   tAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTg                                                                                             >  3:215854/1‑147 (MQ=255)
                                                     aaCCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAg                                                                                          <  6:105737/148‑1 (MQ=255)
                                                          tGAGGCCGGACAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCttt                                                                                    >  1:286722/1‑149 (MQ=255)
                                                            aGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGATTTGTCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTg                                                                                   <  1:76577/148‑1 (MQ=255)
                                                                 ggTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTcc                                                                             >  6:141643/1‑149 (MQ=255)
                                                                   tCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTg                                                                                   >  5:120347/1‑141 (MQ=255)
                                                                   tCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTg                                                                                   <  6:120347/141‑1 (MQ=255)
                                                                     aGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTgg                                                                          <  8:182919/148‑1 (MQ=255)
                                                                         aGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCtt                                                                     >  6:278595/1‑149 (MQ=255)
                                                                         aGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCtt                                                                     <  2:286722/149‑1 (MQ=255)
                                                                            tAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTgg                                                                   <  4:215854/148‑1 (MQ=255)
                                                                             aaaCGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGt                                                                  >  4:159985/1‑148 (MQ=255)
                                                                                       gCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCa                                                       >  5:235261/1‑149 (MQ=255)
                                                                                       gCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCa                                                       >  7:260908/1‑149 (MQ=255)
                                                                                                   ccTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTc                                                                              >  3:228149/1‑114 (MQ=255)
                                                                                                   ccTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTc                                                                              <  4:228149/114‑1 (MQ=255)
                                                                                                               cgcgGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCg                               <  3:128158/149‑1 (MQ=255)
                                                                                                                         gAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTc                     <  7:285808/149‑1 (MQ=255)
                                                                                                                                 aaCGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGt                   >  2:209108/1‑143 (MQ=255)
                                                                                                                                 aaCGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGt                   <  1:209108/143‑1 (MQ=255)
                                                                                                                                      ggCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGGCTCACGAcc        <  6:235261/149‑1 (MQ=255)
                                                                                                                                         ccTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAg     >  8:107195/1‑149 (MQ=255)
                                                                                                                                           tCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGt                                                                  >  7:104097/1‑86 (MQ=38)
                                                                                                                                           tCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGt                                                                  <  8:104097/86‑1 (MQ=38)
                                                                                                                                           tCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGa                 <  2:173954/135‑1 (MQ=255)
                                                                                                                                           tCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGa                 >  1:173954/1‑135 (MQ=255)
                                                                                                                                           tCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGc                                >  2:211676/1‑120 (MQ=255)
                                                                                                                                           tCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGc                                <  1:211676/120‑1 (MQ=255)
                                                                                                                                             agagCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTc   <  5:141643/147‑1 (MQ=255)
                                                                                                                                             agagCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCg  <  5:278595/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
ACTGTAAGTAAATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCG  >  NC_002947/2799028‑2799316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑