Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,804,588 T→C 7.9% L134P (CTG→CCG)  rbsR → DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,804,5880TC 7.9% 143.0 / 2.9 52L134P (CTG→CCG) rbsRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base C (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

CGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATAT  >  NC_002947/2804446‑2804732
                                                                                                                                               |                                                                                                                                                
cgcAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAg                                                                                                                                            <  2:73186/149‑1 (MQ=255)
    aCGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGtgtg                                                                                                                                        >  1:256247/1‑149 (MQ=255)
     cGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTgc                                                                                                                                       >  3:132394/1‑149 (MQ=255)
          aTTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACg                                                                                                                                  >  2:159005/1‑149 (MQ=255)
              cGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCaa                                                                                                                              >  2:164739/1‑149 (MQ=255)
              cGTGATCCTGTGCAACTCAGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCGGCGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCGAGTGTCCGCACGCCaa                                                                                                                              >  2:78171/1‑149 (MQ=255)
               gTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAAt                                                                                                                             <  3:9837/149‑1 (MQ=255)
                      tgtgCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTgcgc                                                                                                                                     >  1:161386/1‑134 (MQ=255)
                      tgtgCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTgcgc                                                                                                                                     <  2:161386/134‑1 (MQ=255)
                          cAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCAtcgtc                                                                                                                   >  6:234201/1‑148 (MQ=255)
                          cAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCAtcgtc                                                                                                                   <  2:256247/148‑1 (MQ=255)
                             cTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGCCGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCAtcg                                                                                                                     >  5:351992/1‑143 (MQ=255)
                             cTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCAtcg                                                                                                                     <  6:351992/143‑1 (MQ=255)
                                cgacgaCAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGt                                                                                                             >  7:102159/1‑148 (MQ=255)
                                    gacAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCt                                                                                                        <  4:132394/149‑1 (MQ=255)
                                    gacAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCt                                                                                                        <  8:102159/149‑1 (MQ=255)
                                         cccGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACAGTGAGCTGGAAg                                                                                                   >  4:235472/1‑149 (MQ=255)
                                          ccGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAgg                                                                                                  >  2:155028/1‑149 (MQ=255)
                                             cAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGt                                                                                               <  1:220881/149‑1 (MQ=255)
                                                aaGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGa                                                                                            >  6:337425/1‑149 (MQ=255)
                                                  gcagcgcagcTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATg                                                                                          <  1:159005/149‑1 (MQ=255)
                                                       gcagcTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGGCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGAc                                                                                     <  7:304497/149‑1 (MQ=255)
                                                        cagcTACCTGCGCGTGCTGCTGGAAAAGCGCCGCGCCGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGGGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGAcc                                                                                    <  6:354704/149‑1 (MQ=255)
                                                           cTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTgg                                                                                 >  3:302551/1‑149 (MQ=255)
                                                            tACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAAACGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGt                                                                                >  1:297206/1‑149 (MQ=255)
                                                             aCCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCAGGGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGt                                                                                >  1:98968/1‑149 (MQ=255)
                                                                           ctgGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGa                                                                 <  5:234201/149‑1 (MQ=255)
                                                                                     gcATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCg                                                       <  2:98968/149‑1 (MQ=255)
                                                                                     gcATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCCGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCg                                                       >  5:190401/1‑149 (MQ=255)
                                                                                     gcATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCCGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCg                                                       >  5:195277/1‑149 (MQ=255)
                                                                                      cATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAgg                                                            <  5:147172/143‑1 (MQ=255)
                                                                                      cATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAgg                                                            >  6:147172/1‑143 (MQ=255)
                                                                                       aTCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTa                                                     >  1:220649/1‑149 (MQ=255)
                                                                                          gACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCCGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCt                                                  <  6:195277/149‑1 (MQ=255)
                                                                                          gACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCCGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCt                                                  <  6:190401/149‑1 (MQ=255)
                                                                                            cGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTgg                                                <  1:155028/149‑1 (MQ=255)
                                                                                                 tGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCAcc                                           >  5:237062/1‑149 (MQ=255)
                                                                                                         gCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATct                                   >  6:344644/1‑149 (MQ=255)
                                                                                                         gCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATct                                   >  5:199746/1‑149 (MQ=255)
                                                                                                           cTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATctgc                                 <  6:237062/149‑1 (MQ=255)
                                                                                                              ggTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATctgctg                               <  8:16528/148‑1 (MQ=255)
                                                                                                                     cA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTgg                       <  3:75753/149‑1 (MQ=255)
                                                                                                                      a‑GGACGACGACGTGCTGCAAAGCCGGGCCAGTGTGCGCACGCCATTGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGGGCATTTACCACGAGCAGGGGGCGTACCTGGCCACACGGCTTGTGCTGGAGCTTGGc                      <  2:297206/149‑1 (MQ=255)
                                                                                                                            gacgacTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTg                        >  8:245499/1‑142 (MQ=255)
                                                                                                                            gacgacTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTg                        <  7:245499/142‑1 (MQ=255)
                                                                                                                            gacgacTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATcg                 <  3:235472/149‑1 (MQ=255)
                                                                                                                              cgacTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATctgc                                 >  2:160341/1‑131 (MQ=255)
                                                                                                                              cgacTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATctgc                                 <  1:160341/131‑1 (MQ=255)
                                                                                                                                     ctgcAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCAtt                                                                         <  4:190699/84‑1 (MQ=255)
                                                                                                                                     ctgcAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCAtt                                                                         >  3:190699/1‑84 (MQ=255)
                                                                                                                                     ctgcAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGc        <  4:302551/149‑1 (MQ=255)
                                                                                                                                           aGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTatat  <  6:199746/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CGCAACGGCTATTGCGTGATCCTGTGCAACTCCGACGACAACCCGCAGAAGCAGCGCAGCTACCTGCGCGTGCTGCTGGAAAAGCGCATCGACGGCCTGGTAGTGGCCTCGGTGGGCCA‑GGACGACGACTTGCTGCAAAGCCTGGCCAGTGTGCGCACGCCAATGGTCATCGTCGACCGTGAGCTGGAAGGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATAT  >  NC_002947/2804446‑2804732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑