Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,808,546 A→T 10.3% noncoding (414/434 nt) PP_mr35 ← cspA regulatory region

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,808,5460AT10.3% 132.0 / 3.6 48noncoding (414/434 nt)PP_mr35cspA regulatory region
Reads supporting (aligned to +/- strand):  ref base A (21/22);  new base T (3/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTT  >  NC_002947/2808405‑2808685
                                                                                                                                             |                                                                                                                                           
tgtgAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCgg                                                                                                                                      <  8:262568/149‑1 (MQ=255)
tgtgAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCgg                                                                                                                                      <  3:183989/149‑1 (MQ=255)
       aGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGtttttt                                                                                                                               <  1:48078/149‑1 (MQ=255)
             cgcgGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACa                                                                                                                           <  4:250393/147‑1 (MQ=255)
                    gCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCCTTGTGGCGGGTTTTTTTat                                                                                                                            <  5:249918/139‑2 (MQ=38)
                    gCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCCTTGTGGCGGGTTTTTTTat                                                                                                                            >  6:249918/1‑138 (MQ=38)
                       ttGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAAt                                                                                                                >  5:138100/1‑148 (MQ=255)
                            ggAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTc                                                                                                           <  3:231731/148‑1 (MQ=255)
                                       aCCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCt                                                                                                <  8:28897/148‑1 (MQ=255)
                                        cccGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCGGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCtgt                                                                                              >  1:224583/1‑149 (MQ=255)
                                         ccGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCtgtg                                                                                             >  1:95820/1‑149 (MQ=255)
                                             gAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGc                                                                                         >  2:216698/1‑149 (MQ=255)
                                                 acGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTg                                                                                      >  6:3703/1‑148 (MQ=255)
                                                   gtgCAAAGACCGTGCCATTCACCCTACAGTCGGTTTCGAATTCAATCCCGCCCCTTCAGATGATGCATAACCCATAAAAAAACCCGACACAAGGGAGGGTTTTTATAAAGCTATCCGAATTACTCCTGACGAAACTGTGCATCCTGCAt                                                                                   >  4:279981/3‑149 (MQ=255)
                                                   gggCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCAt                                                                                   <  5:3703/149‑1 (MQ=255)
                                                         aGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCt                                                                                       >  3:154291/1‑139 (MQ=255)
                                                         aGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCt                                                                                       <  4:154291/139‑1 (MQ=255)
                                                                 ccATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCcg                                                                      <  3:274591/148‑1 (MQ=255)
                                                                      cACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTcc                                                                                                          >  7:231505/1‑107 (MQ=255)
                                                                      cACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTcc                                                                                                          <  8:231505/107‑1 (MQ=255)
                                                                        cccTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCgg                                                               <  7:302509/148‑1 (MQ=255)
                                                                        cccTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCgg                                                               >  8:302509/1‑148 (MQ=255)
                                                                          cTACAGTCGGTTTCGCGGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGcga                                                            <  2:224583/149‑1 (MQ=255)
                                                                            aCAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACACATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGcgac                                                           >  8:98038/1‑148 (MQ=255)
                                                                                    tttCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGaaa                                                   >  3:302154/1‑148 (MQ=255)
                                                                                       cGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGt                                               >  8:265797/1‑149 (MQ=255)
                                                                                          aGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTg                                            >  6:153314/1‑149 (MQ=255)
                                                                                              aaTCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCct                                        >  4:307168/1‑149 (MQ=255)
                                                                                                tCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGcc                                         >  8:135615/1‑146 (MQ=255)
                                                                                                tCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGcc                                         <  7:135615/146‑1 (MQ=255)
                                                                                                  ccGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCcttct                                     <  1:216698/148‑1 (MQ=255)
                                                                                                   cGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCcttctt                                    >  8:45184/1‑148 (MQ=255)
                                                                                                         ttCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGc                              >  4:159816/1‑148 (MQ=255)
                                                                                                          tCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCAACGAAGGAAACAGTCTGGCCTTCttt                                   >  4:8029/1‑142 (MQ=255)
                                                                                                          tCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCAACGAAGGAAACAGTCTGGCCTTCttt                                   <  3:8029/142‑1 (MQ=255)
                                                                                                            aGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCtt                          <  3:307168/149‑1 (MQ=255)
                                                                                                              atgatgCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTg                         >  2:92442/1‑148 (MQ=255)
                                                                                                               tgatgCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGaa                       <  6:138100/149‑1 (MQ=255)
                                                                                                                         cccATAAAACAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCAGAATAACTCCCGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGAt             <  6:167014/149‑1 (MQ=255)
                                                                                                                         cccATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGAt             <  6:171875/149‑1 (MQ=255)
                                                                                                                           tgtAAAAAAACCCGCCCTTGTGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGAt             >  2:260658/3‑147 (MQ=39)
                                                                                                                           tgtAAAAAAACCCGCCCTTGTGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGAt             <  1:260658/145‑1 (MQ=39)
                                                                                                                            aTAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGaa                       <  2:7701/136‑1 (MQ=255)
                                                                                                                            aTAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGaa                       >  1:7701/1‑136 (MQ=255)
                                                                                                                             tAAAAAAACCCGCCCTTGTGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGa         >  7:73223/1‑149 (MQ=39)
                                                                                                                             tAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGa         <  7:45184/149‑1 (MQ=255)
                                                                                                                             tAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGa         >  2:107877/1‑149 (MQ=255)
                                                                                                                                    aCCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCttt  <  7:265797/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                           
TGTGAAAAGCCTGCGCGGTCGCCTTGTAGGAGCGGGTTCACCCGCGAACACGGGCAAAGCCCGTGCCATTCACCCTACAGTCGGTTTCGCAGGCAATCCCGCCCCTTCAGATGATGCATCACCCATAAAAAAACCCGCCACAAGGGCGGGTTTTTTTACAGCTATCCGAATTACTCCGGACGAACCTGTGCAGCCTGCATGCCCTTCTGGCCGCGCTCGGCGACGAAGGAAACAGTCTGGCCTTCTTTCAGGCTCTTGAAGCCGTCAGATTCGATGGCTTT  >  NC_002947/2808405‑2808685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑