| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,829,698 | C→T | 7.4% | A101V (GCC→GTC) | PP_2482 → | molybdenum cofactor biosynthesis protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,829,698 | 0 | C | T | 7.4% | 155.1 / 2.3 | 55 | A101V (GCC→GTC) | PP_2482 | molybdenum cofactor biosynthesis protein A |
| Reads supporting (aligned to +/- strand): ref base C (27/23); new base T (2/2); total (30/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GTTGCTCAGGGATGTTGCAGGGGTTGAGGAACTG‑CGCATTACCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCATTGAGCAGGCGCGCCGAGCC > NC_002947/2829552‑2829843 | gTTGCTCAGGGATGTTGCAGGGGTTGAGGAACTGTCGCATTACCGGCGGCGAGCCGCTGGTGAGTGCCCGGCTGCAGCCGTTTCTGCAGGGCATAGTTAAGATGGCTTTGCAGG‑ACATTAGCATGCCCACCAATGCCCAGTTGCTGGcc > 1:220466/1‑149 (MQ=255)gTTGCTCAGGGATGTTGCAGGGGTTGAGGAACTG‑CGCATTACCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGCAACTGGcca > 6:205344/1‑148 (MQ=255) ttGCTCAGGGATGTTGCAGGGGTTGAGGAACTG‑CGCATTACCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGCAACTGGccat < 5:205344/149‑3 (MQ=255) cTCAGGGATGTCGCAGGGGTTGAGGAACTG‑CGCCTTCCCGGCGGCGAGCCGCTGCTGCGTCACCGGCCGGAGCCCTTTCTGCAGGGCATAGGTCAGCTGGCGTTGCAGG‑ACATTAGCCTCACCACCAATGGCCAGCAACTGGccattgg < 8:299363/149‑6 (MQ=255) aGGGATGTTGCAGGGGTTGAGGAACTG‑CGCATTACCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCaaa < 3:49891/147‑1 (MQ=255) aGGGATGTTGCAGGGGTTGAGGAACTG‑CGCATTACCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACt > 2:209056/1‑149 (MQ=255) ggATGTTGCAGGGGTTGAGGAACTG‑CGCATTACCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGc > 4:298073/1‑149 (MQ=255) gCAGGGGTTGAGGAACTG‑CGCATTACCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTATCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACAAATGGCCAGTAGCTGGACGGCAAACTGACGTTAtt > 7:149328/1‑149 (MQ=255) cAGGGGTTGAGGAACTG‑CGCATTACCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTg < 7:34214/149‑1 (MQ=255) aGGGGTTGAGGAACTG‑CGCATTATCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTg > 8:36023/1‑148 (MQ=255) gAACTG‑CGCATTACCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTATCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCAACAATCGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCgg > 5:96009/1‑149 (MQ=255) cgcATTACCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATc > 7:278663/1‑147 (MQ=255) cgcATTACCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATc < 2:220466/147‑1 (MQ=255) ccGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCCCCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCt > 8:148365/1‑148 (MQ=255) ccGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCt > 6:341155/1‑148 (MQ=255) cggcggCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTc > 6:105913/1‑148 (MQ=255) gcggcgAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAAt < 8:278663/149‑1 (MQ=255) cgAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATgtgt > 1:251619/1‑149 (MQ=255) cgAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATgtgt < 3:298073/149‑1 (MQ=255) gAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCa < 1:11320/121‑1 (MQ=255) gAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCa > 2:11320/1‑121 (MQ=255) gAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCt > 7:198442/1‑129 (MQ=255) gAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCt < 8:198442/129‑1 (MQ=255) aGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCt > 4:311102/1‑149 (MQ=255) aGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCt < 7:36023/149‑1 (MQ=255) gCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGa < 1:209056/149‑1 (MQ=255) cccTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGGTACATTAGCCTGACCACCAATGGCCAGTTGCTGGTCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCTAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCt > 1:234998/1‑149 (MQ=255) cccTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGTCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCtt > 3:48508/1‑149 (MQ=255) cTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTcc < 2:251619/149‑1 (MQ=255) tCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGTCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCTAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGc < 2:234998/148‑1 (MQ=255) tCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGTCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGc < 4:48508/148‑1 (MQ=255) tCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCg < 5:341155/147‑1 (MQ=255) ggCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAATCTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGc > 6:60009/1‑149 (MQ=255) aGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGggcggc > 2:132675/1‑149 (MQ=255) gCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCt > 8:207956/1‑149 (MQ=255) cGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTg < 5:105913/149‑1 (MQ=255) cAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGAc > 4:209313/1‑149 (MQ=255) aGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACg < 3:21372/149‑1 (MQ=255) aCATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTg < 5:222086/149‑1 (MQ=255) aTTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGTCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGc > 4:178086/1‑148 (MQ=255) aTTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCt > 3:284222/1‑149 (MQ=255) ttAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAAtgtg < 2:214845/80‑1 (MQ=255) ttAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAAtgtg > 1:214845/1‑80 (MQ=255) ttAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCt > 5:198334/1‑148 (MQ=255) tGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACgc > 2:295385/1‑147 (MQ=255) gACCACCAATGGCCAGTTGCTGGCCGGCAATCTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCGCTCGATACGCTCGACCCGAGTGCCTTGCGCACGATCGCCAAGGGCGGCGACCTGGAGACGGTGCTCAACGGCa < 8:149328/149‑1 (MQ=255) caccaATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCa < 7:78525/146‑1 (MQ=255) caccaATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCa > 8:78525/1‑146 (MQ=255) caccaATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCAtt > 4:156761/1‑148 (MQ=255) ccaATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCTATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCATTGAg < 6:271516/149‑1 (MQ=255) aTGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGACTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCATTGAGCAg < 6:96009/149‑1 (MQ=255) ccAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCATTGAGCAGgcgc < 7:148365/149‑1 (MQ=255) ccAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCATTGAGCAGgcgc > 6:122046/1‑149 (MQ=255) aGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCATTGAGCAGgcgcgc > 4:238609/1‑149 (MQ=255) gTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCATTGAGCAGGCGCGcc < 1:132675/149‑1 (MQ=255) cTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCATTGAGCAGGCGCGCCGAg < 3:156761/148‑1 (MQ=255) cTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCATTGAGCAGGCGCGCCGAGc < 3:311102/149‑1 (MQ=255) tGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCATTGAGCAGGCGCGCCGAGcc > 5:218941/1‑149 (MQ=255) tGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCATTGAGCAGGCGCGCCGAGcc > 2:259119/1‑149 (MQ=255) | GTTGCTCAGGGATGTTGCAGGGGTTGAGGAACTG‑CGCATTACCGGCGGCGAGCCGCTGCTGAGTGACCGGCTGGAGCCCTTTCTGCAGGGCATAGGTAAGCTGGCGTTGCAGG‑ACATTAGCCTGACCACCAATGGCCAGTTGCTGGCCGGCAAACTGCCGTTATTGCTCAGCGCCGGCATCCAGCGGCTCAATGTGTCCCTCGATACGCTCGACCCGAGTGCCTTCCGCACGATCGCCAAGGGCGGCGACCTGCAGACGGTGCTCAACGGCATTGAGCAGGCGCGCCGAGCC > NC_002947/2829552‑2829843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |