Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,880,316	T→C	6.4%	V53A (GTG→GCG)	PP_2535 →	transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,880,316	0	T	C	6.4%	178.9 / 2.4	62	V53A (GTG→GCG)	PP_2535	transport protein
Reads supporting (aligned to +/- strand):  ref base T (28/30);  new base C (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

AATCCACCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGT  >  NC_002947/2880168‑2880460                                                                                                                                                     |                                                                                                                                                     aaTCCACCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGt                                                                                                                                                      >  6:34733/1‑149 (MQ=255) aaTCCACCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGt                                                                                                                                                      >  7:74231/1‑149 (MQ=255)      aCCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGa                                                                                                                                                 >  1:277410/1‑149 (MQ=255)       ccGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGAt                                                                                                                                                >  2:29658/1‑149 (MQ=255)          cTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCgg                                                                                                                                             <  8:74231/149‑1 (MQ=255)             gTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGgcgc                                                                                                                                          >  2:253806/1‑149 (MQ=255)                gCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGcgcc                                                                                                                                       <  8:50636/149‑1 (MQ=255)                  aCTCGCCATCGGTGCCTTCGGCATCGGAGTTACAGAGTTTGCCCCAATGGGCATGTTGCACAGTATCGCTACAGACCTCGGTGTTTCCATTCATGCTGCGGGCCTGCTGGTCAGTGCCTATGACATCGGCGTGCTGATCTGCGcgacgc                                                                                                                                     >  5:328733/1‑149 (MQ=255)                   cTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCt                                                                                                                                    <  1:253806/149‑1 (MQ=255)                    tCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAgcacgc                                                                                                                                        >  4:292479/1‑144 (MQ=255)                    tCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAgcacgc                                                                                                                                        <  3:292479/144‑1 (MQ=255)                      gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtg‑‑‑‑at                                                                                                                             >  6:330769/1‑149 (MQ=255)                        cATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATCGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGTGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtg‑‑‑‑atga                                                                                                                           <  6:328733/149‑1 (MQ=255)                            ggTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCt                                                                                                                       <  7:320906/149‑1 (MQ=255)                                      gCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGt                                                                                                              >  4:255233/1‑148 (MQ=255)                                        aTCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTg                                                                                                             >  8:204703/1‑147 (MQ=255)                                                 aCAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCcgcg                                                                                                  >  1:181179/1‑149 (MQ=255)                                                 aCAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCcgc                                                                                                   <  5:330769/148‑1 (MQ=255)                                                    gagTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCg                                                                                               >  8:190547/1‑149 (MQ=255)                                                      gTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGtt                                                                                             <  3:255233/149‑1 (MQ=255)                                                       tttGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTa                                                                                            <  2:277410/149‑1 (MQ=255)                                                           cccccATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTg                                                                                        >  2:308385/1‑149 (MQ=255)                                                            ccccATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTgg                                                                                       >  5:302928/1‑148 (MQ=255)                                                            ccccATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGt                                                                                       >  8:289877/1‑149 (MQ=255)                                                              ccATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGtt                                                                                      >  8:221624/1‑148 (MQ=255)                                                               cATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGa                                                                                    <  5:34733/149‑1 (MQ=255)                                                                    gCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCggg                                                                               <  7:204703/149‑1 (MQ=255)                                                                    gCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTCAAGATCCCGCGCCGTTACCTGTTGATCggg                                                                               <  1:29658/149‑1 (MQ=255)                                                                     cATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGc                                                                              <  8:83385/149‑1 (MQ=255)                                                                     cATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGc                                                                              <  4:328679/149‑1 (MQ=255)                                                                     cATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGc                                                                              <  1:308385/149‑1 (MQ=255)                                                                       tGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCt                                                                             >  2:179372/1‑148 (MQ=255)                                                                        gTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGAGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGa                                                                           >  1:76368/1‑149 (MQ=255)                                                                         ttGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCAATCGGCGTGCTGATCGTCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGAt                                                                          <  7:221624/149‑1 (MQ=255)                                                                            cccAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCggg                                                                               <  4:155100/141‑1 (MQ=255)                                                                            cccAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCggg                                                                               >  3:155100/1‑141 (MQ=255)                                                                                gTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCAtt                                                                   >  2:83514/1‑149 (MQ=255)                                                                                 tATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCAttt                                                                  >  8:44641/1‑149 (MQ=255)                                                                                     gCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCAc                                                              >  5:339976/1‑149 (MQ=255)                                                                                       tACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCt                                                            >  3:291139/1‑149 (MQ=255)                                                                                         ccGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTg                                                          <  1:60566/149‑1 (MQ=255)                                                                                         ccGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTg                                                          <  7:289877/149‑1 (MQ=255)                                                                                          cGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                         <  7:44641/149‑1 (MQ=255)                                                                                              cTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGCCACATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                         <  1:280062/149‑1 (MQ=255)                                                                                                cGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGa                                                                                    >  2:177302/1‑116 (MQ=255)                                                                                                 ggTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCt                                                  <  1:83514/149‑1 (MQ=255)                                                                                                        ccATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTc                                            <  4:165550/148‑1 (MQ=255)                                                                                                         cATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCgg                                          <  2:76368/149‑1 (MQ=255)                                                                                                          aTTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGc                                         <  6:339976/149‑1 (MQ=255)                                                                                                          aTTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGc                                         <  1:179372/149‑1 (MQ=255)                                                                                                             ccTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTg                                                 <  7:101094/138‑1 (MQ=255)                                                                                                             ccTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTg                                                 >  8:101094/1‑138 (MQ=255)                                                                                                              ctgctgCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTg                                     >  1:177218/1‑149 (MQ=255)                                                                                                              ctgctgCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTg                                     >  6:110212/1‑149 (MQ=255)                                                                                                                             tgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGc                       >  5:27856/1‑148 (MQ=255)                                                                                                                               gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCa                     <  2:177218/148‑1 (MQ=255)                                                                                                                                          tGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCtggtg          <  5:110212/148‑1 (MQ=255)                                                                                                                                            tcATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGATGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGcc       >  2:325776/2‑149 (MQ=255)                                                                                                                                             cATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGcgc      >  3:10357/1‑147 (MQ=255)                                                                                                                                             cATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGccc      <  7:190547/149‑1 (MQ=255)                                                                                                                                             cATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGccc      >  1:12101/1‑149 (MQ=255)                                                                                                                                               gcGGCGCGCCGATCAGCACGCCGATG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCtggtgg         <  6:324499/143‑1 (MQ=255)                                                                                                                                               gcGGCGCGCCGATCAGCACGCCGATG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCtggtgg         >  5:324499/2‑144 (MQ=255)                                                                                                                                                 ggCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGt  <  6:302928/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     AATCCACCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGT  >  NC_002947/2880168‑2880460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑