Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,880,319	T→C	6.4%	L54P (CTG→CCG)	PP_2535 →	transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,880,319	0	T	C	6.4%	181.9 / 2.1	62	L54P (CTG→CCG)	PP_2535	transport protein
Reads supporting (aligned to +/- strand):  ref base T (28/30);  new base C (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ACCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGT  >  NC_002947/2880173‑2880460                                                                                                                                                   |                                                                                                                                                  aCCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGa                                                                                                                                                 >  1:277410/1‑149 (MQ=255)  ccGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGAt                                                                                                                                                >  2:29658/1‑149 (MQ=255)     cTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCgg                                                                                                                                             <  8:74231/149‑1 (MQ=255)        gTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGgcgc                                                                                                                                          >  2:253806/1‑149 (MQ=255)           gCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGcgcc                                                                                                                                       <  8:50636/149‑1 (MQ=255)             aCTCGCCATCGGTGCCTTCGGCATCGGAGTTACAGAGTTTGCCCCAATGGGCATGTTGCACAGTATCGCTACAGACCTCGGTGTTTCCATTCATGCTGCGGGCCTGCTGGTCAGTGCCTATGACATCGGCGTGCTGATCTGCGcgacgc                                                                                                                                     >  5:328733/1‑149 (MQ=255)              cTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCt                                                                                                                                    <  1:253806/149‑1 (MQ=255)               tCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAgcacgc                                                                                                                                        <  3:292479/144‑1 (MQ=255)               tCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAgcacgc                                                                                                                                        >  4:292479/1‑144 (MQ=255)                 gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtg‑‑‑‑at                                                                                                                             >  6:330769/1‑149 (MQ=255)                   cATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATCGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGTGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtg‑‑‑‑atga                                                                                                                           <  6:328733/149‑1 (MQ=255)                       ggTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCt                                                                                                                       <  7:320906/149‑1 (MQ=255)                                 gCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGt                                                                                                              >  4:255233/1‑148 (MQ=255)                                   aTCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTg                                                                                                             >  8:204703/1‑147 (MQ=255)                                            aCAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCcgcg                                                                                                  >  1:181179/1‑149 (MQ=255)                                            aCAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCcgc                                                                                                   <  5:330769/148‑1 (MQ=255)                                               gagTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCg                                                                                               >  8:190547/1‑149 (MQ=255)                                                 gTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGtt                                                                                             <  3:255233/149‑1 (MQ=255)                                                  tttGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTa                                                                                            <  2:277410/149‑1 (MQ=255)                                                      cccccATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTg                                                                                        >  2:308385/1‑149 (MQ=255)                                                       ccccATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTgg                                                                                       >  5:302928/1‑148 (MQ=255)                                                       ccccATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGt                                                                                       >  8:289877/1‑149 (MQ=255)                                                         ccATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGtt                                                                                      >  8:221624/1‑148 (MQ=255)                                                          cATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGa                                                                                    <  5:34733/149‑1 (MQ=255)                                                               gCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCggg                                                                               <  7:204703/149‑1 (MQ=255)                                                               gCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTCAAGATCCCGCGCCGTTACCTGTTGATCggg                                                                               <  1:29658/149‑1 (MQ=255)                                                                cATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGc                                                                              <  8:83385/149‑1 (MQ=255)                                                                cATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGc                                                                              <  4:328679/149‑1 (MQ=255)                                                                cATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGc                                                                              <  1:308385/149‑1 (MQ=255)                                                                  tGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCt                                                                             >  2:179372/1‑148 (MQ=255)                                                                   gTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGAGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGa                                                                           >  1:76368/1‑149 (MQ=255)                                                                    ttGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCAATCGGCGTGCTGATCGTCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGAt                                                                          <  7:221624/149‑1 (MQ=255)                                                                       cccAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCggg                                                                               <  4:155100/141‑1 (MQ=255)                                                                       cccAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCggg                                                                               >  3:155100/1‑141 (MQ=255)                                                                           gTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCAtt                                                                   >  2:83514/1‑149 (MQ=255)                                                                            tATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCAttt                                                                  >  8:44641/1‑149 (MQ=255)                                                                                gCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCAc                                                              >  5:339976/1‑149 (MQ=255)                                                                                  tACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCt                                                            >  3:291139/1‑149 (MQ=255)                                                                                    ccGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTg                                                          <  1:60566/149‑1 (MQ=255)                                                                                    ccGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTg                                                          <  7:289877/149‑1 (MQ=255)                                                                                     cGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                         <  7:44641/149‑1 (MQ=255)                                                                                         cTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGCCACATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                         <  1:280062/149‑1 (MQ=255)                                                                                           cGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGa                                                                                    >  2:177302/1‑116 (MQ=255)                                                                                            ggTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCt                                                  <  1:83514/149‑1 (MQ=255)                                                                                                   ccATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTc                                            <  4:165550/148‑1 (MQ=255)                                                                                                    cATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCgg                                          <  2:76368/149‑1 (MQ=255)                                                                                                     aTTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGc                                         <  6:339976/149‑1 (MQ=255)                                                                                                     aTTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGc                                         <  1:179372/149‑1 (MQ=255)                                                                                                        ccTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTg                                                 <  7:101094/138‑1 (MQ=255)                                                                                                        ccTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTg                                                 >  8:101094/1‑138 (MQ=255)                                                                                                         ctgctgCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTg                                     >  6:110212/1‑149 (MQ=255)                                                                                                         ctgctgCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTg                                     >  1:177218/1‑149 (MQ=255)                                                                                                                        tgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGc                       >  5:27856/1‑148 (MQ=255)                                                                                                                          gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCa                     <  2:177218/148‑1 (MQ=255)                                                                                                                                     tGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCtggtg          <  5:110212/148‑1 (MQ=255)                                                                                                                                       tcATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGATGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGcc       >  2:325776/2‑149 (MQ=255)                                                                                                                                        cATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGcgc      >  3:10357/1‑147 (MQ=255)                                                                                                                                        cATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGccc      <  7:190547/149‑1 (MQ=255)                                                                                                                                        cATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGccc      >  1:12101/1‑149 (MQ=255)                                                                                                                                          gcGGCGCGCCGATCAGCACGCCGATG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCtggtgg         <  6:324499/143‑1 (MQ=255)                                                                                                                                          gcGGCGCGCCGATCAGCACGCCGATG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCtggtgg         >  5:324499/2‑144 (MQ=255)                                                                                                                                            ggCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGt  <  6:302928/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ACCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTG‑‑‑‑ATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGT  >  NC_002947/2880173‑2880460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑