Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,922,538 C→T 8.8% R905R (AGG→AGA)  PP_2563 ← antibiotic biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,922,5380CT 8.8% 156.8 / 5.0 57R905R (AGG→AGA) PP_2563antibiotic biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base C (24/28);  new base T (2/3);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

CCACTTCCGCCAGGTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGAT  >  NC_002947/2922394‑2922681
                                                                                                                                                |                                                                                                                                               
ccacTTCCGCCAGGTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGTCTGc                                                                                                                                             >  4:230742/1‑149 (MQ=255)
  acTTCCGCCAGGTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCgg                                                                                                                                           <  5:38006/149‑1 (MQ=255)
         acAGGTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAca                                                                                                                                    <  4:194389/148‑1 (MQ=255)
           aGGTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAcagc                                                                                                                                  <  2:124257/149‑1 (MQ=255)
             gTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAcagcag                                                                                                                                >  5:75966/1‑149 (MQ=255)
               caccacCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCt                                                                                                                                               <  5:249020/132‑1 (MQ=255)
               caccacCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCt                                                                                                                                               >  6:249020/1‑132 (MQ=255)
                accaccTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACg                                                                                                                             <  7:152852/149‑1 (MQ=255)
                  caccTGGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGc                                                                                                                            >  7:306667/1‑148 (MQ=255)
                  caccTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGc                                                                                                                            >  8:159361/1‑148 (MQ=255)
                  caccTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGc                                                                                                                            >  2:189822/1‑148 (MQ=255)
                    ccTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGTCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGTCTGCTGTTTACCCGCAGGCGCaag                                                                                                                         <  3:135205/149‑2 (MQ=255)
                    ccTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGTCTGCTGTTTACCCGCAGGCGCaag                                                                                                                         <  3:230742/149‑2 (MQ=255)
                     cTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCaa                                                                                                                          >  2:332462/1‑147 (MQ=255)
                       cGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGTCTGCTGTTTACCCGCAGGCGCaag                                                                                                                         >  2:241677/1‑145 (MQ=255)
                       cGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGTCTGCTGTTTACCCGCAGGCGCaag                                                                                                                         <  1:241677/146‑2 (MQ=255)
                       cGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAgg                                                                                                                      <  1:249051/149‑1 (MQ=255)
                            cAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGtt                                                                                                                 <  7:192321/149‑1 (MQ=255)
                                     gctgAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCaa                                                                                                          >  3:62750/1‑147 (MQ=255)
                                     gctgAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATa                                                                                                        <  4:62750/149‑1 (MQ=255)
                                            tGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAcagc                                                                                                                                  <  3:245805/116‑1 (MQ=255)
                                            tGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAcagc                                                                                                                                  >  4:245805/1‑116 (MQ=255)
                                                       gAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCCCGCGCCTGATGCTGCTTGCGCCTGCGGGTata                                                                                                                                      >  6:108340/1‑99 (MQ=255)
                                                          tagtGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCATCAATAGCTCACCAGTACCCCTCCAGc                                                                                   <  1:332462/149‑1 (MQ=255)
                                                            gtGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGc                                                                                   >  1:55180/1‑147 (MQ=255)
                                                             tGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAg                                                                                >  4:169908/1‑149 (MQ=255)
                                                                    gcTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAgg                                                                         >  5:332499/1‑149 (MQ=255)
                                                                        gCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAACAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTa                                                                     >  3:323784/1‑149 (MQ=255)
                                                                          gggTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTAtc                                                                   >  4:176188/1‑149 (MQ=255)
                                                                          gggTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTAACCAGTACCCCTCCAGCGAGTGCCAGGGCTAtc                                                                   >  4:135237/1‑149 (MQ=255)
                                                                               aTCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAgg                                                                                                                      <  2:226786/93‑1 (MQ=255)
                                                                               aTCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAgg                                                                                                                      >  1:226786/1‑93 (MQ=255)
                                                                                tCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCTGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAAAAGCTCAACAGAACCCCTGCAGGGAGTGCCAGGGCTAtcgagaa                                                              >  8:285277/1‑143 (MQ=255)
                                                                                    cGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGc                                                          <  3:176188/148‑1 (MQ=255)
                                                                                      ttCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCggg                                                       <  5:346701/149‑1 (MQ=255)
                                                                                      ttCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCggg                                                       >  6:286298/1‑149 (MQ=255)
                                                                                       tCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAca                                                                                                                                    >  5:14881/1‑71 (MQ=255)
                                                                                       tCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAca                                                                                                                                    <  6:14881/71‑1 (MQ=255)
                                                                                       tCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTc                                                                                       >  5:77233/1‑116 (MQ=255)
                                                                                       tCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTc                                                                                       <  6:77233/116‑1 (MQ=255)
                                                                                                ggTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGaaaa                                             <  6:332499/149‑1 (MQ=255)
                                                                                                  tGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACa                                           <  5:286298/149‑1 (MQ=255)
                                                                                                  tGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACa                                           <  3:135237/149‑1 (MQ=255)
                                                                                                      gcgTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAACAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCt                                       <  4:323784/149‑1 (MQ=255)
                                                                                                            aGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCt                                  <  7:159361/148‑1 (MQ=255)
                                                                                                                 gATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTc                                      >  5:122175/1‑139 (MQ=255)
                                                                                                                 gATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTc                                      <  6:122175/139‑1 (MQ=255)
                                                                                                                      gCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCAc                                                                                                  <  7:159533/74‑1 (MQ=255)
                                                                                                                      gCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCAc                                                                                                  >  8:159533/1‑74 (MQ=255)
                                                                                                                         ccACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCGACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTg                    >  6:244644/1‑149 (MQ=255)
                                                                                                                          cACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTg                    <  1:189822/148‑1 (MQ=255)
                                                                                                                             gatccTGATGATGCTTGCGCATGAGGGTAAACAGCAGTCGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACACCTCCAGCTAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGaaa                <  6:239407/146‑1 (MQ=255)
                                                                                                                             ctgcCTGTTGCGTCTGGCGCCTGCGTGTAAACCGCAGACGCAACAGGGTGTGCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGaaa                <  6:239405/147‑1 (MQ=255)
                                                                                                                                ccTGATGCTGCTTGCGCCTGCTGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGc             <  8:53514/149‑1 (MQ=255)
                                                                                                                                   gATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACa                                           <  2:101084/116‑1 (MQ=255)
                                                                                                                                   gATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACa                                           >  1:101084/1‑116 (MQ=255)
                                                                                                                                    aTGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCa         <  6:75966/149‑1 (MQ=255)
                                                                                                                                             gcgcCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGat  <  8:248485/147‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CCACTTCCGCCAGGTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGAT  >  NC_002947/2922394‑2922681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑