Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,922,543 G→T 9.9% R904S (CGC→AGC)  PP_2563 ← antibiotic biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,922,5430GT 9.9% 157.3 / 4.5 56R904S (CGC→AGC) PP_2563antibiotic biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (23/27);  new base T (2/4);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTCCGCCAGGTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACAA  >  NC_002947/2922396‑2922687
                                                                                                                                                   |                                                                                                                                                
acTTCCGCCAGGTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCgg                                                                                                                                                 <  5:38006/149‑1 (MQ=255)
       acAGGTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAca                                                                                                                                          <  4:194389/148‑1 (MQ=255)
         aGGTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAcagc                                                                                                                                        <  2:124257/149‑1 (MQ=255)
           gTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAcagcag                                                                                                                                      >  5:75966/1‑149 (MQ=255)
              accaccTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACg                                                                                                                                   <  7:152852/149‑1 (MQ=255)
                caccTGGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGc                                                                                                                                  >  7:306667/1‑148 (MQ=255)
                caccTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGc                                                                                                                                  >  2:189822/1‑148 (MQ=255)
                caccTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGc                                                                                                                                  >  8:159361/1‑148 (MQ=255)
                  ccTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGTCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGTCTGCTGTTTACCCGCAGGCGCaag                                                                                                                               <  3:135205/149‑2 (MQ=255)
                  ccTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGTCTGCTGTTTACCCGCAGGCGCaag                                                                                                                               <  3:230742/149‑2 (MQ=255)
                   cTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCaa                                                                                                                                >  2:332462/1‑147 (MQ=255)
                     cGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGTCTGCTGTTTACCCGCAGGCGCaag                                                                                                                               <  1:241677/146‑2 (MQ=255)
                     cGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGTCTGCTGTTTACCCGCAGGCGCaag                                                                                                                               >  2:241677/1‑145 (MQ=255)
                     cGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAgg                                                                                                                            <  1:249051/149‑1 (MQ=255)
                          cAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGtt                                                                                                                       <  7:192321/149‑1 (MQ=255)
                                   gctgAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCaa                                                                                                                >  3:62750/1‑147 (MQ=255)
                                   gctgAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATa                                                                                                              <  4:62750/149‑1 (MQ=255)
                                          tGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAcagc                                                                                                                                        <  3:245805/116‑1 (MQ=255)
                                          tGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAcagc                                                                                                                                        >  4:245805/1‑116 (MQ=255)
                                                     gAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCCCGCGCCTGATGCTGCTTGCGCCTGCGGGTata                                                                                                                                            >  6:108340/1‑99 (MQ=255)
                                                        tagtGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCATCAATAGCTCACCAGTACCCCTCCAGc                                                                                         <  1:332462/149‑1 (MQ=255)
                                                          gtGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGc                                                                                         >  1:55180/1‑147 (MQ=255)
                                                           tGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAg                                                                                      >  4:169908/1‑149 (MQ=255)
                                                                  gcTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAgg                                                                               >  5:332499/1‑149 (MQ=255)
                                                                      gCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAACAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTa                                                                           >  3:323784/1‑149 (MQ=255)
                                                                        gggTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTAtc                                                                         >  4:176188/1‑149 (MQ=255)
                                                                        gggTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTAACCAGTACCCCTCCAGCGAGTGCCAGGGCTAtc                                                                         >  4:135237/1‑149 (MQ=255)
                                                                             aTCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAgg                                                                                                                            <  2:226786/93‑1 (MQ=255)
                                                                             aTCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAgg                                                                                                                            >  1:226786/1‑93 (MQ=255)
                                                                              tCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCTGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAAAAGCTCAACAGAACCCCTGCAGGGAGTGCCAGGGCTAtcgagaa                                                                    >  8:285277/1‑143 (MQ=255)
                                                                                  cGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGc                                                                <  3:176188/148‑1 (MQ=255)
                                                                                    ttCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCggg                                                             <  5:346701/149‑1 (MQ=255)
                                                                                    ttCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCggg                                                             >  6:286298/1‑149 (MQ=255)
                                                                                     tCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAca                                                                                                                                          >  5:14881/1‑71 (MQ=255)
                                                                                     tCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAAca                                                                                                                                          <  6:14881/71‑1 (MQ=255)
                                                                                     tCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTc                                                                                             <  6:77233/116‑1 (MQ=255)
                                                                                     tCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTc                                                                                             >  5:77233/1‑116 (MQ=255)
                                                                                              ggTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGaaaa                                                   <  6:332499/149‑1 (MQ=255)
                                                                                                tGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACa                                                 <  3:135237/149‑1 (MQ=255)
                                                                                                tGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACa                                                 <  5:286298/149‑1 (MQ=255)
                                                                                                    gcgTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAACAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCt                                             <  4:323784/149‑1 (MQ=255)
                                                                                                          aGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCt                                        <  7:159361/148‑1 (MQ=255)
                                                                                                               gATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTc                                            <  6:122175/139‑1 (MQ=255)
                                                                                                               gATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTc                                            >  5:122175/1‑139 (MQ=255)
                                                                                                                    gCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCAc                                                                                                        >  8:159533/1‑74 (MQ=255)
                                                                                                                    gCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCAc                                                                                                        <  7:159533/74‑1 (MQ=255)
                                                                                                                       ccACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCGACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTg                          >  6:244644/1‑149 (MQ=255)
                                                                                                                        cACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTg                          <  1:189822/148‑1 (MQ=255)
                                                                                                                           gatccTGATGATGCTTGCGCATGAGGGTAAACAGCAGTCGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACACCTCCAGCTAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGaaa                      <  6:239407/146‑1 (MQ=255)
                                                                                                                           ctgcCTGTTGCGTCTGGCGCCTGCGTGTAAACCGCAGACGCAACAGGGTGTGCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGaaa                      <  6:239405/147‑1 (MQ=255)
                                                                                                                              ccTGATGCTGCTTGCGCCTGCTGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGc                   <  8:53514/149‑1 (MQ=255)
                                                                                                                                 gATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACa                                                 <  2:101084/116‑1 (MQ=255)
                                                                                                                                 gATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACa                                                 >  1:101084/1‑116 (MQ=255)
                                                                                                                                  aTGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCa               <  6:75966/149‑1 (MQ=255)
                                                                                                                                           gcgcCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGat        <  8:248485/147‑1 (MQ=255)
                                                                                                                                               cTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATAc    >  7:318696/1‑147 (MQ=255)
                                                                                                                                               cTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACaa  <  2:55180/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
ACTTCCGCCAGGTCACCACCTCGGCCCAGGTGCTGGCTGAACTGGCCGGACGCGAGTAGTGCATGCGCTGGCGGGTCATCTTCGTTCAGGCAGAGGTGGCGCGTTCAGCTGGATCGGCTCCACGCGCCTGATGCTGCTTGCGCCTGCGGGTAAACAGCAGACGCAACAGGGTGTTCCAGCAATAGCTCACCAGTACCCCTCCAGCGAGTGCCAGGGCTATCTCGAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACAA  >  NC_002947/2922396‑2922687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑