Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,923,326 C→A 8.5% A643S (GCG→TCG)  PP_2563 ← antibiotic biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,923,3260CA 8.5% 144.3 / 2.4 47A643S (GCG→TCG) PP_2563antibiotic biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base C (26/17);  new base A (2/2);  total (28/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

CAGCAGCAGCGATTGCCAGTGCAGCCGGGCGAATACCAGCTGGATCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATGCGGATGAACACCGGCATTTCCATTACCCGCCACGCGCCTTTG  >  NC_002947/2923180‑2923474
                                                                                                                                                  |                                                                                                                                                    
cagcagcagCGATTGCCAGTGCAGCCGGGCGAATACCAGCTGTATCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAgcgcgc                                                                                                                                                      >  8:132891/1‑147 (MQ=255)
cagcagcagCGATTGCCAGTGCAGCCGGGCGAATACCAGCTGGATCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAgcgcgc                                                                                                                                                      >  2:46125/1‑147 (MQ=255)
      cagcGATTGCCAGTGCAGCCGGGCGAATACCAGCTGGATCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGggcga                                                                                                                                              >  1:161319‑M2/1‑144 (MQ=255)
       agcGATTGCCAGTGCAGCCGGGCGAATACCAGCTGGATCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGggcgat                                                                                                                                             >  1:46125‑M2/1‑143 (MQ=255)
        gcGATTGCCAGTGCAGCCGGGCGAATACCAGCTGGATCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGggcgatg                                                                                                                                            >  2:304607‑M2/1‑142 (MQ=255)
               ccAGTGCAGCCGGGCGAATACCAGCTGGATCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGgaga                                                                                                                                     <  7:175594/149‑1 (MQ=255)
                 aGTGCAGCCGGGCGAATACCAGCTGGATCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGAcc                                                                                                                                   <  4:199426/149‑1 (MQ=255)
                                aTACCAGCTGGATCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAg                                                                                                                    >  2:56177/1‑149 (MQ=255)
                                aTACCAGCTGGATCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAg                                                                                                                    <  2:166659/149‑1 (MQ=255)
                                    cAGCTGGATCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGttt                                                                                                                >  7:19388/1‑149 (MQ=255)
                                           aTCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGa                                                                                                          <  2:42253/148‑1 (MQ=255)
                                             cAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGCACAGCACCGAGGTTTCGCCGAGTa                                                                                                       >  3:75262/1‑149 (MQ=255)
                                               aTGCGCTGTACAACAGGGTTGGCACCACGATGGCAGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATc                                                                                                     >  1:225989/1‑149 (MQ=255)
                                                tGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCg                                                                                                    <  2:225989/149‑1 (MQ=255)
                                                          aacaGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCgg                                                                                                   >  5:19196/1‑140 (MQ=255)
                                                          aacaGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCgg                                                                                                   <  6:19196/140‑1 (MQ=255)
                                                          aacaGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTc                                                                                          >  6:116407/1‑149 (MQ=255)
                                                          aacaGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGAATCGCCACTCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTACGCCGAGTATCGGCCAGTggac                                                                                          >  1:14734/1‑147 (MQ=255)
                                                             aGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGa                                                                                        >  6:18198/1‑148 (MQ=255)
                                                                    gCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCAcc                                                                                 <  7:132891/148‑1 (MQ=255)
                                                                      accacGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAg                                                                              >  4:117190/1‑149 (MQ=255)
                                                                        cacGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGt                                                                            >  2:268010/1‑149 (MQ=255)
                                                                         acGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTg                                                                           >  2:92303/1‑149 (MQ=255)
                                                                            aTGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGcc                                                                        <  5:116407/149‑1 (MQ=255)
                                                                                 tGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGAcc                                                                    >  1:312567/1‑148 (MQ=255)
                                                                                 tGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACca                                                                   >  2:316677/1‑149 (MQ=255)
                                                                                      gTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATc                                                              <  5:18198/149‑1 (MQ=255)
                                                                                          ccTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGga                                                          <  3:117190/149‑1 (MQ=255)
                                                                                           cTGAGCAGCGTGGGTGTATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGTCCACAATCAGgat                                                         <  2:14734/149‑1 (MQ=255)
                                                                                                   cgtgcgtgCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGc                                                   >  2:252183/1‑147 (MQ=255)
                                                                                                   cgtgcgtgCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGa                                                 <  1:268010/149‑1 (MQ=255)
                                                                                                    gtgcgtgcATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGggcgatgcacgcacgctgctcaggccactgacagccatcgtggtgccaaccctgttgtacagcgcattgatccagctggtattcgcccggctgcactgg                                                <  5:22317‑M2/149‑100 (MQ=255)
                                                                                                    gtgcgtgcATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAg                                                >  4:242622/1‑149 (MQ=255)
                                                                                                     tgcgtgcATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGACAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAg                                                >  2:272350/1‑148 (MQ=255)
                                                                                                     tgcgtgcATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGACAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAg                                                >  2:268125/1‑148 (MQ=255)
                                                                                                      gcgtgcATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCa                                              >  2:221948/1‑149 (MQ=255)
                                                                                                          gcATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATCCg                                          >  5:10717/1‑149 (MQ=255)
                                                                                                          gcATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACAAGGAACAGCACAGAGGTTTCGCCGAGTATCGGACAGTGGTCGAAATCAACCAGGTGGCAGACAACAATCAGGATGATGGCg                                                  >  1:246464/1‑141 (MQ=255)
                                                                                                           cATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATGCgg                                         <  2:312567/149‑1 (MQ=255)
                                                                                                                 cACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGggcgatgcacgcacgctgctcaggccactgacagccatcgtggtgccaaccctgttgtaca                                                                                      >  7:61795‑M2/1‑37 (MQ=255)
                                                                                                                 cACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGggcgatgcacgcacgctgctcaggccactgacagccatcgtggtgccaaccctgttgtaca                                                                                      <  8:61795‑M2/98‑62 (MQ=255)
                                                                                                                                ccTTGAGCTGAAAGCGCGACAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATGCGGATGAACACCGGCATTTCCAtt                    <  1:272350/149‑1 (MQ=255)
                                                                                                                                ccTTGAGCTGAAAGCGCGACAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATGCGGATGAACACCGGCATTTCCAtt                    <  1:268125/149‑1 (MQ=255)
                                                                                                                                    gAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATGCGGATGAACACCGGCATTTCCATTAccc                >  2:139644/1‑149 (MQ=255)
                                                                                                                                     aGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCa                                                             <  5:316300/103‑1 (MQ=255)
                                                                                                                                     aGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCa                                                             >  6:316300/1‑103 (MQ=255)
                                                                                                                                        tGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATGCGGATGAACACCGGCATTTCCATTACCCGCCa            >  2:237280/1‑149 (MQ=255)
                                                                                                                                          aaaGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATGCGGATGAACACCg                               >  7:155070/1‑128 (MQ=255)
                                                                                                                                          aaaGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATGCGGATGAACACCg                               <  8:155070/128‑1 (MQ=255)
                                                                                                                                               gcgcCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATGCGGATGAACACCg                               <  4:19439/123‑1 (MQ=255)
                                                                                                                                                 gcCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATGCGGATGAACACCGGCATTTCCATTACCCGCCACGCGCCttt   <  3:193882/149‑1 (MQ=255)
                                                                                                                                                  ccAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATGCGGATGAACACCGGCATTTCCATTACCCGCCACGCGCCTTTg  <  1:237280/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CAGCAGCAGCGATTGCCAGTGCAGCCGGGCGAATACCAGCTGGATCAATGCGCTGTACAACAGGGTTGGCACCACGATGGCTGTCAGTGGCCTGAGCAGCGTGCGTGCATCGCCACGCGCTTCGATGGCCTTGAGCTGAAAGCGCGCCAGGGCGATGCCGGAGACCAGGAACAGCACCGAGGTTTCGCCGAGTATCGGCCAGTGGTCGAAATCACCCAGGTGGCCGACCACAATCAGGATGATGGCGAGCATGCGGATGAACACCGGCATTTCCATTACCCGCCACGCGCCTTTG  >  NC_002947/2923180‑2923474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑