Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,941,331 T→A 11.3% L188Q (CTG→CAG)  PP_2574 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,941,3310TA11.3% 88.6 / 2.6 35L188Q (CTG→CAG) PP_2574LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (16/15);  new base A (2/2);  total (18/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTGACCGCTGGAACATTCGACCTGGCATTTCTCTATGACCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATGGTCG  >  NC_002947/2941184‑2941474
                                                                                                                                                   |                                                                                                                                               
gCCTGACCGCTGGAACATTCGACCTGGCATTTCTCTATGACCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTg                                                                                                                                                <  3:44396/149‑1 (MQ=255)
              aCATGCGACCAGGCATTTCTCACTGACCACGACCTGGATGGCACTCTCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGccc                                                                                                                                  <  4:218815/149‑1 (MQ=255)
                            aTTTCTCTATGACCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCAGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTCTGCCCGGAGCCGATGATc                                                                                                                     >  5:350402/1‑148 (MQ=255)
                                ctctATGACCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCctgc                                                                                                                 <  6:354172/148‑1 (MQ=255)
                                   tATGACCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGa                                                                                                             >  5:248386/1‑149 (MQ=255)
                                       aCCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTg                                                                                                         >  8:148221/1‑149 (MQ=255)
                                         cACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGc                                                                                                       >  1:322452/1‑149 (MQ=255)
                                                              gAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGt                                                                                  <  1:303107/149‑1 (MQ=255)
                                                                      gCCGCTGATGCCGCCTCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTg                                                                           <  6:248386/148‑1 (MQ=255)
                                                                        cGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCACACGTACTTTGTCAGCTTGTTc                                                                        <  8:216887/149‑1 (MQ=255)
                                                                             aTGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAAc                                                                     <  6:350402/147‑1 (MQ=255)
                                                                                 cgccgcAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGtt                                                                         >  5:177523/1‑139 (MQ=255)
                                                                                 cgccgcAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGtt                                                                         <  6:177523/139‑1 (MQ=255)
                                                                                  gccgcAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATgg                                                              >  6:324704/1‑149 (MQ=255)
                                                                                  gccgcAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATgg                                                              >  3:155577/1‑149 (MQ=255)
                                                                                    cgcAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGc                                                            <  5:324704/149‑1 (MQ=255)
                                                                                                          aCCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTg                                                         >  2:149424/1‑130 (MQ=255)
                                                                                                          aCCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTg                                                         <  1:149424/130‑1 (MQ=255)
                                                                                                               agaACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGccc                                                                                                     <  7:140822/81‑1 (MQ=255)
                                                                                                               agaACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGccc                                                                                                     >  8:140822/1‑81 (MQ=255)
                                                                                                                   ccaccGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCtt                                        >  1:208710/1‑138 (MQ=255)
                                                                                                                   ccaccGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCtt                                        <  2:208710/138‑1 (MQ=255)
                                                                                                                      ccGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGtcga                          >  1:345678/1‑149 (MQ=255)
                                                                                                                        gCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGtcgatc                        >  2:150072/1‑149 (MQ=255)
                                                                                                                          tttGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGtcgatc                        >  1:81418/1‑147 (MQ=255)
                                                                                                                            tGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCgag                     <  4:155577/148‑1 (MQ=255)
                                                                                                                                 tccgggCACAGGTCGCGCAGCGAGACCTGGGCCTGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTg               >  3:326162/5‑149 (MQ=38)
                                                                                                                                     ggCACAGGTCGCGCAGCGAGACCTGGGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGcgcg           >  3:198392/1‑149 (MQ=255)
                                                                                                                                        ccAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCa        >  5:232796/1‑149 (MQ=255)
                                                                                                                                        acAGGTCGCGCAGCGAGACCTGGGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCa        <  4:198392/148‑1 (MQ=255)
                                                                                                                                          aGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATg      >  4:16371/1‑149 (MQ=255)
                                                                                                                                           ggTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATgg     >  2:282795/1‑149 (MQ=255)
                                                                                                                                           ggTCGCGCAGCGAGACCTGGGCCTGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATgg     <  4:326162/149‑1 (MQ=255)
                                                                                                                                            gTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATggt    <  2:345678/149‑1 (MQ=255)
                                                                                                                                                 gCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATggtcg  <  3:16371/146‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GCCTGACCGCTGGAACATTCGACCTGGCATTTCTCTATGACCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATGGTCG  >  NC_002947/2941184‑2941474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑