| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,941,342 | T→G | 11.4% | C192G (TGC→GGC) | PP_2574 → | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,941,342 | 0 | T | G | 11.4% | 90.8 / 4.8 | 35 | C192G (TGC→GGC) | PP_2574 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (16/15); new base G (2/2); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01 | |||||||||||
ACATTCGACCTGGCATTTCTCTATGACCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATGGTCGGTCAGG > NC_002947/2941198‑2941480 | aCATGCGACCAGGCATTTCTCACTGACCACGACCTGGATGGCACTCTCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGccc < 4:218815/149‑1 (MQ=255) aTTTCTCTATGACCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCAGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTCTGCCCGGAGCCGATGATc > 5:350402/1‑148 (MQ=255) ctctATGACCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCctgc < 6:354172/148‑1 (MQ=255) tATGACCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGa > 5:248386/1‑149 (MQ=255) aCCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTg > 8:148221/1‑149 (MQ=255) cACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGc > 1:322452/1‑149 (MQ=255) gAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGt < 1:303107/149‑1 (MQ=255) gCCGCTGATGCCGCCTCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTg < 6:248386/148‑1 (MQ=255) cGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCACACGTACTTTGTCAGCTTGTTc < 8:216887/149‑1 (MQ=255) aTGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAAc < 6:350402/147‑1 (MQ=255) cgccgcAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGtt < 6:177523/139‑1 (MQ=255) cgccgcAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGtt > 5:177523/1‑139 (MQ=255) gccgcAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATgg > 6:324704/1‑149 (MQ=255) gccgcAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATgg > 3:155577/1‑149 (MQ=255) cgcAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGc < 5:324704/149‑1 (MQ=255) aCCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTg > 2:149424/1‑130 (MQ=255) aCCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTg < 1:149424/130‑1 (MQ=255) agaACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGccc < 7:140822/81‑1 (MQ=255) agaACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGccc > 8:140822/1‑81 (MQ=255) ccaccGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCtt > 1:208710/1‑138 (MQ=255) ccaccGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCtt < 2:208710/138‑1 (MQ=255) ccGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGtcga > 1:345678/1‑149 (MQ=255) gCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGtcgatc > 2:150072/1‑149 (MQ=255) tttGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGtcgatc > 1:81418/1‑147 (MQ=255) tGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCgag < 4:155577/148‑1 (MQ=255) tccgggCACAGGTCGCGCAGCGAGACCTGGGCCTGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTg > 3:326162/5‑149 (MQ=38) ggCACAGGTCGCGCAGCGAGACCTGGGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGcgcg > 3:198392/1‑149 (MQ=255) ccAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCa > 5:232796/1‑149 (MQ=255) acAGGTCGCGCAGCGAGACCTGGGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCa < 4:198392/148‑1 (MQ=255) aGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATg > 4:16371/1‑149 (MQ=255) ggTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATgg > 2:282795/1‑149 (MQ=255) ggTCGCGCAGCGAGACCTGGGCCTGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATgg < 4:326162/149‑1 (MQ=255) gTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATggt < 2:345678/149‑1 (MQ=255) gCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATggtcg < 3:16371/146‑1 (MQ=255) cgcgACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATGGTCGGTCAgg < 2:81418/148‑1 (MQ=255) | ACATTCGACCTGGCATTTCTCTATGACCACGACCTGGATGGCACTATCGAGGCCGAGCCGCTGATGCCGCCGCAGAAGCCCTATGTGCTGCTACCCGAGAACCACCGCTTTGCCAGCCAGGCCCAGGTCTCGCTGCGCGACCTGTGCCCGGAGCCGATGATCCTGCTGGACGTGGCCCCTAGCCGCACGTACTTTGTCAGCTTGTTCAACGAGATGGGCCTGGCACCGAACATCGTCTTCAGTTCACCGTCGATCGAGATGGTGCGCGGCATGGTCGGTCAGG > NC_002947/2941198‑2941480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |