Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,951,341 A→C 16.1% intergenic (‑120/+41) oguA ← / ← PP_2585 8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,951,3410AC16.1% 101.1 / 11.1 43intergenic (‑120/+41)oguA/PP_25858‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (18/18);  new base C (4/3);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTG  >  NC_002947/2951203‑2951483
                                                                                                                                          |                                                                                                                                              
cAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCGCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGaa                                                                                                                                      <  6:73786/149‑1 (MQ=255)
 aGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCcgcg                                                                                                                                        >  2:173632/1‑146 (MQ=255)
 aGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGAGCCGGCCTCTTGGCGGCTAGAGCCGCGAag                                                                                                                                     <  1:29644/149‑1 (MQ=255)
         tAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCgg                                                                                                                              <  2:177081/148‑1 (MQ=255)
                        aaGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAaga                                                                                                                                    <  3:201913/127‑1 (MQ=255)
                        aaGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAaga                                                                                                                                    >  4:201913/1‑127 (MQ=255)
                              gcgcAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGa                                                                                                            >  5:100806/1‑145 (MQ=255)
                                    tACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCacac                                                                                                  <  1:173632/149‑1 (MQ=255)
                                    tACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCacac                                                                                                  <  2:201110/149‑1 (MQ=255)
                                               gATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGgc                                                                                       >  1:10689/1‑149 (MQ=255)
                                                    ccGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTTCTCAATGTATACAGCTCGAAGTGCCCGTGCAGTCCTCTTGGCGGCTAGAGCCGCGAAGAGCCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTc                                                                                  >  2:25589/1‑149 (MQ=255)
                                                            aTAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTc                                                                          <  2:10689/149‑1 (MQ=255)
                                                            aTAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTc                                                                          >  2:39505/1‑149 (MQ=255)
                                                             tAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGcc                                                                                                                                           <  4:105312/83‑1 (MQ=255)
                                                              aGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCa                                                                        >  4:145800/1‑149 (MQ=255)
                                                               ggCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAg                                                                       >  3:149059/1‑149 (MQ=255)
                                                                gcgcTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCGGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGAt                                                                                                       <  1:192865/116‑1 (MQ=255)
                                                                gcgcTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGAt                                                                                                       >  2:192865/1‑116 (MQ=255)
                                                                  gcTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCaa                                                                    >  7:178384/1‑149 (MQ=255)
                                                                            gTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAAGAGGCCGGCACgggc                                                                                                                       <  3:164059/88‑2 (MQ=18)
                                                                            gTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCCGCCTCTTAGCGGGTCTAGCCGCCAAGAGGCCGGCCCgggc                                                                                                                       >  4:164059/1‑87 (MQ=21)
                                                                             tAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATcc                                                         >  2:236368/1‑149 (MQ=255)
                                                                                gcaagcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATAACACCGCCACCCGGCTGCTACCACCCTCCAGCAACCCGGCaaaacccc                                                      >  2:118108/1‑143 (MQ=255)
                                                                                  aagcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAg                                                                       >  5:230340/1‑130 (MQ=255)
                                                                                  aagcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAg                                                                       <  6:230340/130‑1 (MQ=255)
                                                                                    gcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCgg                                                  >  7:111479/1‑149 (MQ=255)
                                                                                            cTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGt                                          <  3:145800/149‑1 (MQ=255)
                                                                                               aTTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGtttt                                       >  6:5526/1‑149 (MQ=255)
                                                                                                ttGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGttttt                                      <  6:100806/149‑1 (MQ=255)
                                                                                                   tataCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCaa                                   >  7:53635/1‑149 (MQ=255)
                                                                                                     taCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAAc                                  <  1:236368/148‑1 (MQ=255)
                                                                                                          cTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGgctgc                                                                                    <  3:266138/93‑1 (MQ=255)
                                                                                                          cTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGgctgc                                                                                    >  4:266138/1‑93 (MQ=255)
                                                                                                             gAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCCCACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTGAACGCATCCGgc                         >  6:27928/1‑149 (MQ=255)
                                                                                                                         ccGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCa             <  8:53635/149‑1 (MQ=255)
                                                                                                                         ccGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCa             <  8:178384/149‑1 (MQ=255)
                                                                                                                           gggcactTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAgg           <  1:125705/144‑1 (MQ=255)
                                                                                                                           ggCCTCTTTGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAgg           <  1:118108/149‑1 (MQ=255)
                                                                                                                             ccTCTTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCCCCCCCTCCAGCACCCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGGCAggg          >  5:112895/1‑148 (MQ=38)
                                                                                                                              ctctTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGTGTTGCAGCCATTAAAGATCACACCTCCTCGAGGCTGATCACACCCTCCAGCAACCGGGCAAACACAAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAggg          >  6:350723/1‑147 (MQ=255)
                                                                                                                               tctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTACAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAgg           <  7:240193/145‑1 (MQ=39)
                                                                                                                               tctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTACAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAgg           >  8:240193/1‑145 (MQ=39)
                                                                                                                                    gcggcTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTg  <  8:111479/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                              
CAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTG  >  NC_002947/2951203‑2951483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑