Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,951,349	G→C	17.1%	intergenic (‑128/+33)	oguA ← / ← PP_2585	8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,951,349	0	G	C	17.1%	89.6 / 13.5	41	intergenic (‑128/+33)	oguA/PP_2585	8‑oxoguanine deaminase/alpha‑ketoglutaric semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (17/17);  new base C (4/3);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01

CAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTGGAAGCACACCGGG  >  NC_002947/2951203‑2951496                                                                                                                                                   |                                                                                                                                                    cAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCGCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGaa                                                                                                                                                   <  6:73786/149‑1 (MQ=255)  aGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCcgcg                                                                                                                                                     >  2:173632/1‑146 (MQ=255)  aGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGAGCCGGCCTCTTGGCGGCTAGAGCCGCGAag                                                                                                                                                  <  1:29644/149‑1 (MQ=255)          tAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCgg                                                                                                                                           <  2:177081/148‑1 (MQ=255)                         aaGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAaga                                                                                                                                                 >  4:201913/1‑127 (MQ=255)                         aaGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAaga                                                                                                                                                 <  3:201913/127‑1 (MQ=255)                               gcgcAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGa                                                                                                                         >  5:100806/1‑145 (MQ=255)                                     tACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCacac                                                                                                               <  1:173632/149‑1 (MQ=255)                                     tACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCacac                                                                                                               <  2:201110/149‑1 (MQ=255)                                                gATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGgc                                                                                                    >  1:10689/1‑149 (MQ=255)                                                     ccGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTTCTCAATGTATACAGCTCGAAGTGCCCGTGCAGTCCTCTTGGCGGCTAGAGCCGCGAAGAGCCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTc                                                                                               >  2:25589/1‑149 (MQ=255)                                                             aTAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTc                                                                                       <  2:10689/149‑1 (MQ=255)                                                             aTAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTc                                                                                       >  2:39505/1‑149 (MQ=255)                                                               aGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCa                                                                                     >  4:145800/1‑149 (MQ=255)                                                                ggCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAg                                                                                    >  3:149059/1‑149 (MQ=255)                                                                 gcgcTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCGGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGAt                                                                                                                    <  1:192865/116‑1 (MQ=255)                                                                 gcgcTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGAt                                                                                                                    >  2:192865/1‑116 (MQ=255)                                                                   gcTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCaa                                                                                 >  7:178384/1‑149 (MQ=255)                                                                             gTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTCGCGGCTCTAGCCGCCAAGAGGCCGGCACgggc                                                                                                                                    <  3:164059/88‑2 (MQ=18)                                                                             gTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCCGCCTCTTAGCGGGTCTAGCCGCCAAGAGGCCGGCCCgggc                                                                                                                                    >  4:164059/1‑87 (MQ=21)                                                                              tAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATcc                                                                      >  2:236368/1‑149 (MQ=255)                                                                                 gcaagcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATAACACCGCCACCCGGCTGCTACCACCCTCCAGCAACCCGGCaaaacccc                                                                   >  2:118108/1‑143 (MQ=255)                                                                                   aagcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAg                                                                                    <  6:230340/130‑1 (MQ=255)                                                                                   aagcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAg                                                                                    >  5:230340/1‑130 (MQ=255)                                                                                     gcTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCgg                                                               >  7:111479/1‑149 (MQ=255)                                                                                             cTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGt                                                       <  3:145800/149‑1 (MQ=255)                                                                                                aTTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGtttt                                                    >  6:5526/1‑149 (MQ=255)                                                                                                 ttGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGttttt                                                   <  6:100806/149‑1 (MQ=255)                                                                                                    tataCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCaa                                                >  7:53635/1‑149 (MQ=255)                                                                                                      taCAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAAc                                               <  1:236368/148‑1 (MQ=255)                                                                                                           cTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGgctgc                                                                                                 >  4:266138/1‑93 (MQ=255)                                                                                                           cTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGgctgc                                                                                                 <  3:266138/93‑1 (MQ=255)                                                                                                              gAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCCCACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTGAACGCATCCGgc                                      >  6:27928/1‑149 (MQ=255)                                                                                                                          ccGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCa                          <  8:53635/149‑1 (MQ=255)                                                                                                                          ccGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCa                          <  8:178384/149‑1 (MQ=255)                                                                                                                            gggcactTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAgg                        <  1:125705/144‑1 (MQ=255)                                                                                                                            ggCCTCTTTGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAgg                        <  1:118108/149‑1 (MQ=255)                                                                                                                              ccTCTTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCCCCCCCTCCAGCACCCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGGCAggg                       >  5:112895/1‑148 (MQ=38)                                                                                                                               ctctTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGTGTTGCAGCCATTAAAGATCACACCTCCTCGAGGCTGATCACACCCTCCAGCAACCGGGCAAACACAAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAggg                       >  6:350723/1‑147 (MQ=255)                                                                                                                                tctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTACAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAgg                        <  7:240193/145‑1 (MQ=39)                                                                                                                                tctTCGCGGCTCTAGCCGCCAAGAGGCCGGCACGGGTTACAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAgg                        >  8:240193/1‑145 (MQ=39)                                                                                                                                     gcggcTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTg               <  8:111479/149‑1 (MQ=255)                                                                                                                                                  cgAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTGGAAGCACACCggg  >  7:325587/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CAGGGTTTGTAGATGACATGGTGCAAGCCTGCGCAATACGCATCGGCGATTACCGATGTCATAGGCGCTCGGTATTGTAAGCAAGCTTTTTACTGATTGTATACAGCTCGAAGTGCCCGTGCCGGCCTCTTGGCGGCTAGAGCCGCGAAGAGGCCGGCACGGGTTGCAGCCATGAAAGATCACACCGCCTCGCGGCTGCTCACACCCTCCAGCAACCGGGCAATCCCCAGCGGGTTAGCGTTTTTCAACGCATCCGGCAGCAGTGCGTCAGGGTAGTTCTGGAAGCACACCGGG  >  NC_002947/2951203‑2951496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑