Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 296,371 T→G 6.5% E19A (GAG→GCG)  PP_0242 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947296,3710TG 6.5% 229.1 / 4.5 73E19A (GAG→GCG) PP_0242TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (33/35);  new base G (3/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGCCTGG  >  NC_002947/296225‑296517
                                                                                                                                                  |                                                                                                                                                   
tAAGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTc                                                                                                                                                    >  2:59179/1‑148 (MQ=255)
  aGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGAt                                                                                                                                                 >  6:116934/1‑149 (MQ=255)
      ccTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCAATcctg                                                                                                                                             >  6:118661/1‑149 (MQ=255)
       cTCCAGCAAGGCAGGCAGGCTTGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGAGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctgg                                                                                                                                            <  8:307869/149‑1 (MQ=255)
          cAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATcctggcct                                                                                                                                         >  2:184953/1‑149 (MQ=255)
                  ccGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGAGGCGATcctggcg                                                                                                                                          <  5:34234/140‑2 (MQ=255)
                  ccGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGAGGCGATcctggcg                                                                                                                                          >  6:34234/1‑139 (MQ=255)
                   cGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTgg                                                                                                                                >  1:327076/1‑149 (MQ=255)
                           tGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGgc                                                                                                                               >  1:253737/1‑142 (MQ=255)
                           tGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGgc                                                                                                                               <  2:253737/142‑1 (MQ=255)
                             gCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGttt                                                                                                                      <  1:184953/149‑1 (MQ=255)
                             gCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGttt                                                                                                                      >  4:64748/1‑149 (MQ=255)
                               gAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTgc                                                                                                                    >  1:139537/1‑149 (MQ=255)
                                  aaaGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGcgcg                                                                                                                 <  2:139537/149‑1 (MQ=255)
                                    aGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCggg                                                                                                               <  1:345217/149‑1 (MQ=255)
                                            acCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggtggt                                                                                                       >  6:40669/1‑149 (MQ=255)
                                             ccGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTATATACAACGGGCTGCCCCCCCTTGTTCGGCCAACCGCGCACGCGCAGCCGCCAGCATCGCCGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGgtggtggtg                                                                                                      <  1:90008/149‑1 (MQ=255)
                                              cgacgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGc                                                                                                     <  5:40669/149‑1 (MQ=255)
                                               gacgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGCATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCg                                                                                                    <  8:203379/149‑1 (MQ=255)
                                                acgaAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCgg                                                                                                   >  1:177289/1‑149 (MQ=255)
                                                   aaGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTtc                                                                                                <  7:86876/149‑1 (MQ=255)
                                                     ggCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCACGATCGCCTCGATCCTGGCCTGGCTGCTGCCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTtca                                                                                               >  5:165067/1‑148 (MQ=255)
                                                         gAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAAc                                                                                         >  2:271491/1‑149 (MQ=255)
                                                           aTCTGCGCCCGCTGGGACACGCTGTAGATAGATAGTGATGCGAACCATTTTTCGTCAATCAGCTCACGCTCAGCCGCCATGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAAct                                                                                        <  8:281247/148‑1 (MQ=255)
                                                              tGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAActgct                                                                                     >  3:123663/1‑148 (MQ=255)
                                                                cgcCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAAc                                                                                         <  2:211493/142‑1 (MQ=255)
                                                                cgcCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCAGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAActagatcc                                                                                  >  1:211493/1‑143 (MQ=255)
                                                                 gcCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGAtt                                                                                 <  5:116934/149‑1 (MQ=255)
                                                                  cccGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTg                                                                                >  8:292386/1‑149 (MQ=255)
                                                                  cccGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTg                                                                                >  5:85079/1‑149 (MQ=255)
                                                                         ggCCACGCTGTAGATAGTAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAgg                                                                           <  6:330984/147‑1 (MQ=255)
                                                                            cACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCt                                                                      >  4:11325/1‑149 (MQ=255)
                                                                               gCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGgctg                                                                                                                                    >  7:156305/1‑85 (MQ=255)
                                                                               gCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGgctg                                                                                                                                    <  8:156305/85‑1 (MQ=255)
                                                                                     gatagaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTa                                                             <  1:59179/149‑1 (MQ=255)
                                                                                         gaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAAc                                                                                         <  6:190930/117‑1 (MQ=255)
                                                                                         gaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGgcg                                                         >  2:28030/1‑149 (MQ=255)
                                                                                         gaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGgcg                                                         >  3:102370/1‑149 (MQ=255)
                                                                                         gaAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTAACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAAc                                                                                         >  5:190930/1‑117 (MQ=255)
                                                                                          aaaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTAAT‑CAACAGATGATTGCCAGGATGATGGCCGGGTAGgcgc                                                        >  8:215103/1‑149 (MQ=255)
                                                                                           aaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGttt                                                                                                                      <  6:277253/87‑1 (MQ=255)
                                                                                           aaGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGttt                                                                                                                      >  5:277253/1‑87 (MQ=255)
                                                                                            aGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCcg                                                      >  4:217482/1‑149 (MQ=255)
                                                                                             gTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCcga                                                     <  6:85079/149‑1 (MQ=255)
                                                                                                  gcCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGTATGTTGGCCTGGTAGGCGCCGACGAAc                                                <  7:215103/149‑1 (MQ=255)
                                                                                                   ccACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAAc                                                <  6:303663/148‑1 (MQ=255)
                                                                                                     aCCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAg                                              >  3:32083/1‑148 (MQ=255)
                                                                                                     aCCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAg                                              <  4:32083/148‑1 (MQ=255)
                                                                                                      ccccTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGt                                            <  3:11325/149‑1 (MQ=255)
                                                                                                        ccTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCg                                          <  3:64748/149‑1 (MQ=255)
                                                                                                          ttGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCGGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCg                                          >  1:223742/1‑147 (MQ=255)
                                                                                                                 gCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggtg                                                                                                         <  5:23898/78‑1 (MQ=255)
                                                                                                                 gCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGggtggtg                                                                                                         >  6:23898/1‑78 (MQ=255)
                                                                                                                  ccAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCATCCAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCg                                 <  1:271491/149‑1 (MQ=255)
                                                                                                                   cAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGAc                               <  3:217482/149‑1 (MQ=255)
                                                                                                                         cTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCGGTGACTTCgac                         >  8:160032/1‑147 (MQ=255)
                                                                                                                          tCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGttt                        >  2:339839/1‑149 (MQ=255)
                                                                                                                            aCGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAg                                              >  6:79361/1‑125 (MQ=255)
                                                                                                                            aCGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAg                                              <  5:79361/125‑1 (MQ=255)
                                                                                                                            aCGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCGGTGACTTCgacctg                      <  7:160032/149‑6 (MQ=255)
                                                                                                                            aCGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGttt                        <  2:177289/147‑1 (MQ=255)
                                                                                                                            aCGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTg                      >  4:286070/1‑149 (MQ=255)
                                                                                                                            aCGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCGGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGttt                        <  2:223742/147‑1 (MQ=255)
                                                                                                                                    ccgccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCt              <  2:327076/149‑1 (MQ=255)
                                                                                                                                    aggccAGGATCGAGGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCt              >  4:226195/3‑149 (MQ=255)
                                                                                                                                     ggccAGGATCGAGGCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTc             >  6:283467/2‑149 (MQ=255)
                                                                                                                                       ccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCgg           <  2:185430/149‑1 (MQ=255)
                                                                                                                                       ccAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCgg           <  4:123663/149‑1 (MQ=255)
                                                                                                                                        cAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGa          <  3:286070/149‑1 (MQ=255)
                                                                                                                                          ggATCGCCTCAATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACt        <  5:118661/149‑1 (MQ=255)
                                                                                                                                             tCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCcgac                                                    <  4:316912/102‑1 (MQ=255)
                                                                                                                                             tCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCcgac                                                    >  3:316912/1‑102 (MQ=255)
                                                                                                                                                ccTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGCCTgg  >  8:283522/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TAAGTGCCTCCAGCAAGGCCGGCACGCTGGCGAAAAAGTGATACACCGACGAAGGCGGAATCTGCGCCCGCTCGGCCACGCTGTAGATAGAAAGTGCTGCCACCCCTTGTTCGGCCAACAGCTCACGCGCAGCCGCCAGGATCGCCTCGATCCTGGCCTGGCTGCTGGCGCGTGGTTTGCGCGGGGTGGTGGTGCGGTTCAT‑CAACTGCTGATTGCCAGGATGCTGGCCTGGTAGGCGCCGACGAACAGGTCGAAGTCACCGACTTCGTTTTGCTCCAGCTCGGACTGCCTGG  >  NC_002947/296225‑296517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑