Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,007,081	G→A	10.0%	intergenic (+41/+37)	PP_2627 → / ← PP_2628	membrane protein/ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,007,081	0	G	A	10.0%	136.6 / 5.3	50	intergenic (+41/+37)	PP_2627/PP_2628	membrane protein/ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (23/22);  new base A (3/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

TTACCCAGCAACCTTTCAAAGGCTTCCGCTTGCCCCGAGGCTTGTTGCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACAGTTCGGCATGGGTGCC  >  NC_002947/3006940‑3007228                                                                                                                                              |                                                                                                                                                     ttACCCAGCAACCTTTCAAAGGCTTCCGCTTGCCCCGAGGCTTGTTGCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTg                                                                                                                                               <  8:121009/149‑1 (MQ=255)    cccAGCAACCTTTCAAAGGCTTCCGCTTGCCCCGAGGCTTGTTGCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGt                                                                                                                                            <  2:305921/149‑1 (MQ=255)          aaCCTTTCAAAGGCTTCCGCTTGCCCCGAGGCTTGTTGCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATgcgc                                                                                                                                      >  5:56134/1‑149 (MQ=255)             cTTTCAAAGGCTTCCGCTTGCCCCGAGGCTTGTTGCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCgctgg                                                                                                                                   >  2:142412/1‑149 (MQ=255)              tttCAAAGGCTTCCGCTTGCCCCGAGGCTTGTTGCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCt                                                                                                                                                >  2:97314/1‑135 (MQ=255)              tttCAAAGGCTTCCGCTTGCCCCGAGGCTTGTTGCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCt                                                                                                                                                <  1:97314/135‑1 (MQ=255)                        ttCCGCTTGCCCCGAGGCTTGTTGCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGc                                                                                                                        >  1:181749/1‑149 (MQ=255)                                gCCCCGAGGCTTGTTGCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGc                                                                                                                  <  1:142412/147‑1 (MQ=255)                                          ttgttgCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑c                                                                                                             <  3:6379/141‑1 (MQ=255)                                          ttgttgCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑c                                                                                                             >  4:6379/1‑141 (MQ=255)                                          ttgttgCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGc                                                                                                     >  2:110167/1‑149 (MQ=255)                                             ttgCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGc                                                                                                                                                  >  8:314051/1‑102 (MQ=255)                                             ttgCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGc                                                                                                                                                  <  7:314051/102‑1 (MQ=255)                                             ttgCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCcac                                                                                                        <  7:15588/143‑1 (MQ=255)                                             ttgCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCcac                                                                                                        >  8:15588/1‑143 (MQ=255)                                               gCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCg                                                                                                 <  6:56134/148‑1 (MQ=255)                                                    agccagccAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCAc                                                                                            <  7:277479/148‑1 (MQ=255)                                                    agccagccAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCAc                                                                                            >  8:277479/1‑148 (MQ=255)                                                                tggtgCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCcaca                                                                                                       <  7:25149/125‑1 (MQ=255)                                                                tggtgCAGGCGGTGCTACCGGATGGCCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCcaca                                                                                                       >  8:25149/1‑125 (MQ=255)                                                                   tgCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTACCTCCACAGCCTCGGCCACGCCCTGATCCTCAcc                                                                             <  1:40941/149‑1 (MQ=255)                                                                    gCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGTCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCATCAcc                                                                             >  8:71079/1‑147 (MQ=255)                                                                    gCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCg                                                                                                 >  4:229208/1‑127 (MQ=255)                                                                    gCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCg                                                                                                 <  3:229208/127‑1 (MQ=255)                                                                         cGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAAc                                                                      <  2:181749/149‑1 (MQ=255)                                                                                    gATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCActct                                                            >  3:312455/1‑148 (MQ=255)                                                                                       gggCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGAtg                                                        >  3:86606/1‑149 (MQ=255)                                                                                         gCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGAtgct                                                      >  3:273498/1‑149 (MQ=255)                                                                                         gCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGAtgct                                                      <  5:63006/149‑1 (MQ=255)                                                                                                cTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCAccc                                                                            <  1:244434/120‑1 (MQ=255)                                                                                                cTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCAccc                                                                            >  2:244434/1‑120 (MQ=255)                                                                                                 tATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAAc                                              >  1:130256/1‑149 (MQ=255)                                                                                                                  gcgcAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCtt                             >  5:277094/1‑149 (MQ=255)                                                                                                                     cAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACTGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCttttt                          <  1:110167/149‑1 (MQ=255)                                                                                                                          cATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGcc                      <  6:277094/148‑1 (MQ=255)                                                                                                                            ttGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCCCCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCttttt                          <  2:35120/142‑1 (MQ=255)                                                                                                                            ttGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCTCAACCGTGCATAGGTACCCTTTTTCGCCaa                    >  5:61393/1‑148 (MQ=255)                                                                                                                            ttGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCttttt                          >  1:35120/1‑142 (MQ=255)                                                                                                                                  acccAGGCTTCGAAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACa                  <  3:13459/141‑1 (MQ=255)                                                                                                                                  acccAGGCTTCGAAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACa                  >  4:13459/4‑144 (MQ=255)                                                                                                                                    ccAGGCTTCGAAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCCCTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACAGTTCgg            >  2:166690/2‑148 (MQ=255)                                                                                                                                    ccAGGCTTCGAAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGAtgctgc                                                    >  3:71236/2‑108 (MQ=255)                                                                                                                                    ccAGGCTTCGAAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGAtgctgc                                                    <  4:71236/107‑1 (MQ=255)                                                                                                                                      aGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCTCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACAGTTCGGCAt         <  3:335792/149‑1 (MQ=255)                                                                                                                                      aGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACGCAGTATCCCGCCAAGCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACAGTTCGGCAt         <  5:239656/149‑1 (MQ=255)                                                                                                                                        gCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACAGTTCGGCATgg       <  2:130256/149‑1 (MQ=255)                                                                                                                                          tttCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACAGTTCGGCATgg       >  3:62317/1‑147 (MQ=255)                                                                                                                                          tttCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACAGTTCGGCATGGGt     >  8:175721/1‑149 (MQ=255)                                                                                                                                          tttCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACAGTTCGGCATGGGt     >  1:205592/1‑149 (MQ=255)                                                                                                                                            tCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACAGTTCGGCATgg       <  4:62317/145‑1 (MQ=255)                                                                                                                                              gAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGc                                                                  <  6:210392/84‑1 (MQ=255)                                                                                                                                              gAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGc                                                                  >  5:210392/1‑84 (MQ=255)                                                                                                                                              gAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACAGTTCGGCATGGGTGcc  <  4:312455/148‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                     TTACCCAGCAACCTTTCAAAGGCTTCCGCTTGCCCCGAGGCTTGTTGCAACAGCCAGCCAGTGTGGTGCAGGCGGTGCTACCGGATGGGCAGCGACTATGAACCTCGTCAGGGGCGCAGTGCATTGACTGTGCAGGCTTTCGAAGCCTGGGTATGCGCTGGCTGCCCCCGGCCTCTGCTTA‑CTCCACAGCCTCGGCCACGCCCTGATCCTCACCCAGGAACCCGCCACTCTGATGCTGCCACAACCGTGCATAGGTACCCTTTTTCGCCAACAGTTCGGCATGGGTGCC  >  NC_002947/3006940‑3007228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑