Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,057,280 G→T 10.6% A23D (GCC→GAC)  PP_2669 ← outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,057,2800GT10.6% 166.9 / 7.4 56A23D (GCC→GAC) PP_2669outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (27/23);  new base T (3/3);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGTCGTGTGACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTC  >  NC_002947/3057134‑3057425
                                                                                                                                                   |                                                                                                                                                 
tGCTGTCGTGTGACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGc                                                                                                                                                  >  1:11761/1‑148 (MQ=255)
 gCTGTCGTGTGACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGc                                                                                                                                                  >  6:168447/1‑147 (MQ=255)
   tGTCGTGTGACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAgc                                                                                                                                              >  4:211342/1‑149 (MQ=255)
       gtgtGACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGcc                                                                                                                                           <  2:11761/148‑1 (MQ=255)
              agcagcAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCGTTCTCGTTGGAGACCCCGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGcc                                                                                                                                   >  6:208737/1‑149 (MQ=255)
               gcagcaAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGccc                                                                                                                                  <  3:113472/149‑1 (MQ=255)
                   caAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGa                                                                                                                                >  1:5537/1‑147 (MQ=255)
                   caAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGAca                                                                                                                              <  5:168447/149‑1 (MQ=255)
                           gcgGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTg                                                                                                                      <  8:169049/149‑1 (MQ=255)
                                  cTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTa                                                                                                                 >  8:130603/1‑147 (MQ=255)
                                     gCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTGCACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGc                                                                                                             <  1:76128/149‑1 (MQ=255)
                                       ccACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAgg                                                                                                          >  1:207648/1‑149 (MQ=255)
                                       ccACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAgg                                                                                                          <  3:202702/149‑1 (MQ=255)
                                         aCCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGc                                                                                                        >  5:60169/1‑149 (MQ=255)
                                          ccGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGcc                                                                                                       >  4:141414/1‑149 (MQ=255)
                                            ggCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCg                                                                                                     >  5:44213/1‑149 (MQ=255)
                                               aGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTg                                                                                                  >  3:266268/1‑149 (MQ=255)
                                                        gTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAgggg                                                                                         >  4:103998/1‑149 (MQ=255)
                                                        gTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAgggg                                                                                         <  2:207648/149‑1 (MQ=255)
                                                         tGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAggggg                                                                                        >  2:19055/1‑149 (MQ=255)
                                                           aCTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGggcg                                                                                      <  6:60169/149‑1 (MQ=255)
                                                             ttCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGggcggc                                                                                    >  2:92656/1‑149 (MQ=255)
                                                             ttCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGggcggc                                                                                    >  5:194758/1‑149 (MQ=255)
                                                                               gATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTa                                                                   <  3:211342/148‑1 (MQ=255)
                                                                                   aggctgaggctgTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTAttgtt                                                              >  7:59846/1‑149 (MQ=255)
                                                                                      ctgaggctgTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTtgtg                                                           <  1:19055/149‑1 (MQ=255)
                                                                                      ctgaggctgTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTtgtg                                                           <  5:94627/149‑1 (MQ=255)
                                                                                               ttgtCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCgg                                                   <  7:130603/148‑1 (MQ=255)
                                                                                                  tCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGt                                                 >  3:35187/1‑147 (MQ=255)
                                                                                                     ttCTCGTTGGAGACACAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAggg                                            >  7:150997/1‑149 (MQ=255)
                                                                                                       ctcGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAggg                                            <  3:141414/147‑1 (MQ=255)
                                                                                                          gTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTa                                         <  1:92656/147‑1 (MQ=255)
                                                                                                          gTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGc                                       <  3:103998/149‑1 (MQ=255)
                                                                                                          gTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTCTTGTGGTTGTCGGGTAAGGGGTAGc                                       >  6:269943/1‑149 (MQ=255)
                                                                                                               agaCCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTacac                                  <  4:35187/149‑1 (MQ=255)
                                                                                                                   ccAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCa                              >  1:85173/1‑149 (MQ=255)
                                                                                                                    cAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCAc                             <  3:118529/149‑1 (MQ=255)
                                                                                                                       gCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTc                          >  3:323457/1‑149 (MQ=255)
                                                                                                                           tGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGc                      <  5:208737/149‑1 (MQ=255)
                                                                                                                             gCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCtt                    >  1:125485/1‑149 (MQ=255)
                                                                                                                              cAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTg                   <  6:44213/149‑1 (MQ=255)
                                                                                                                               aGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTgg                                                          <  2:261234/109‑1 (MQ=255)
                                                                                                                                 ccATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGt                                <  8:186039/133‑1 (MQ=255)
                                                                                                                                 ccATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGt                                >  7:186039/1‑133 (MQ=255)
                                                                                                                                   aTGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCa                >  3:77636/1‑147 (MQ=255)
                                                                                                                                      gtgtG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGcc           >  4:308571/1‑149 (MQ=255)
                                                                                                                                       tgtg‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGcc           >  6:134257/1‑148 (MQ=255)
                                                                                                                                        ccagcctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGcc           >  4:37918/6‑148 (MQ=38)
                                                                                                                                           gcctgctgTCGGGCGCCCTGGCGCAGGCCAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCtt                    <  3:290165/134‑1 (MQ=255)
                                                                                                                                           gcctgctgTCGGGCGCCCTGGCGCAGGCCAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCtt                    >  4:290165/3‑136 (MQ=255)
                                                                                                                                            aCTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAgg       <  8:59846/148‑1 (MQ=255)
                                                                                                                                             ctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTacac                                  >  2:187571/1‑120 (MQ=38)
                                                                                                                                             ctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTacac                                  <  1:187571/120‑1 (MQ=38)
                                                                                                                                             ctgctgTCGGGCGCCCTGGCGCTGGCCAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCt     <  3:37918/149‑1 (MQ=255)
                                                                                                                                                ctgGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTc  <  6:194758/149‑1 (MQ=255)
                                                                                                                                                 tgGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGATTGTCATAGCCCAGGCTGTc  >  7:27473/1‑148 (MQ=255)
                                                                                                                                                   gCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTac                                    <  2:158273/112‑1 (MQ=255)
                                                                                                                                                   gCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTac                                    >  1:158273/1‑112 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGCTGTCGTGTGACAGCAGCAAGCCACGCGGGCGCTGGCCCACCGGCAGGGTTTCGGTGACTTCCAGCGTTTGCAGGTCGATCAGGCTGAGGCTGTTGTCCTTCTCGTTGGAGACCCAGGCGGTGGCAGCCATGGTGTG‑ACTGCTGGCCAGCGCCAGGGCGCCCGACAGCAGGCTGGGGTAAAGCAGGGCCCGGTGGAAGAGGGGGCGGCGCATGGCGGATTCCTTATTGTTGTGGTTGTCGGGTCAGGGGTAGCTACACGTCACTTCGGGCTTGTCATAGCCCAGGCTGTC  >  NC_002947/3057134‑3057425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑