Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,065,552	G→C	9.5%	G164G (GGG→GGC) ‡	PP_2677 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,065,552	0	G	C	9.5%	180.3 / 9.6	63	G164G (GGG→GGC) ‡	PP_2677	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base C (4/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

TCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATGGCCAGCG  >  NC_002947/3065407‑3065695                                                                                                                                                  |                                                                                                                                                tcGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAg                                                                                                                                              <  3:253243/149‑1 (MQ=255)     aCAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGc                                                                                                                                          >  7:191282/1‑149 (MQ=255)     aCAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGc                                                                                                                                          >  2:8617/1‑149 (MQ=255)        ggCCACTCCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTgg                                                                                                                                       <  8:243337/149‑1 (MQ=255)         gCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTggg                                                                                                                                      >  3:666/1‑149 (MQ=255)           cacaCCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTgggcg                                                                                                                                    >  1:61111/1‑149 (MQ=255)                cGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGgcgc                                                                                                                               >  5:17978/1‑149 (MQ=255)                           gcTGTACTGCCGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTCTTCATTGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTgg                                                                                                                    <  1:288051/149‑1 (MQ=255)                                agcggcgcgcgATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGAca                                                                                                               <  2:124095/144‑1 (MQ=255)                                 cTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGAcag                                                                                                              >  1:244650/1‑149 (MQ=255)                                 cTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGAcag                                                                                                              >  3:289350/1‑149 (MQ=255)                                    aCGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGAcagca                                                                                                            <  3:81969/148‑1 (MQ=255)                                        gcgATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAgccgc                                                                                                       >  2:87428/1‑149 (MQ=255)                                           aTGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctg                                                                                                    >  2:139086/1‑149 (MQ=255)                                            tGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgc                                                                                                   >  2:40188/1‑149 (MQ=255)                                            tGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgc                                                                                                   <  8:191282/149‑1 (MQ=255)                                              gCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcgc                                                                                                 >  4:123378/1‑149 (MQ=255)                                                cTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcgcct                                                                                               <  5:58560/149‑1 (MQ=255)                                                cTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcgcct                                                                                               >  4:48046/1‑149 (MQ=255)                                                   tgGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCcgcctgcgcctg                                                                                              <  1:87428/147‑1 (MQ=255)                                                   tgGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGATGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCtcctgcgcctgc                                                                                             <  7:66902/148‑1 (MQ=255)                                                     gCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAg                                                                                           <  2:61111/148‑1 (MQ=255)                                                             gTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCAc                                                                                  >  2:187914/1‑149 (MQ=255)                                                                 gACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCa                                                                              <  4:666/149‑1 (MQ=255)                                                                  aCCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCAt                                                                             >  5:172105/1‑149 (MQ=255)                                                                  aCCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCAt                                                                             >  7:202148/1‑149 (MQ=255)                                                                  aCCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCAt                                                                             <  1:8617/149‑1 (MQ=255)                                                                      tGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGa                                                                          >  7:296942/1‑148 (MQ=255)                                                                         tCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAAc                                                                      <  2:244650/149‑1 (MQ=255)                                                                               cggccggcGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTg                                                                <  7:140009/149‑1 (MQ=255)                                                                                   cggcggcggTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGt                                                              >  4:61737/1‑147 (MQ=255)                                                                                           gTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGc                                                                                      >  4:121072/1‑115 (MQ=255)                                                                                           gTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGc                                                                                      <  3:121072/115‑1 (MQ=255)                                                                                           gTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACg                                                                     <  3:92586/132‑1 (MQ=255)                                                                                           gTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACg                                                                     >  4:92586/1‑132 (MQ=255)                                                                                            ttGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTc                                                   <  3:61737/149‑1 (MQ=255)                                                                                                 ccGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGa                                               <  1:40188/148‑1 (MQ=255)                                                                                                   gCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGtt                                            <  6:17978/149‑1 (MQ=255)                                                                                                       ccAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttcttt                                        >  8:92785/1‑149 (MQ=255)                                                                                                         aGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttctttct                                      >  8:324612/1‑149 (MQ=255)                                                                                                          gCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCGGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTtctc                                     <  4:289350/149‑1 (MQ=255)                                                                                                          gCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGttctttct                                      >  2:269039/1‑148 (MQ=255)                                                                                                              ggtgCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAAcc                                 <  6:172105/149‑1 (MQ=255)                                                                                                                   gcACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGcc                            <  7:324612/149‑1 (MQ=255)                                                                                                                    cACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGcc                            >  6:200629/1‑148 (MQ=255)                                                                                                                            ccGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTAc                     >  3:291853/1‑147 (MQ=255)                                                                                                                             cGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAg                  <  8:296942/149‑1 (MQ=255)                                                                                                                             cGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAg                  <  4:291853/149‑1 (MQ=255)                                                                                                                               gCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGt                <  7:92785/149‑1 (MQ=255)                                                                                                                                  ccgcccagCACCTCCCCGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCCGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTa                      >  7:93831/7‑140 (MQ=255)                                                                                                                                  ccgcccagCACCTCCCCGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCCGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTa                      <  8:93831/134‑1 (MQ=255)                                                                                                                                   ggaggaGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTg               >  3:138377/1‑146 (MQ=255)                                                                                                                                    gaggagCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGTGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTg               >  2:72334/1‑145 (MQ=255)                                                                                                                                    gaggagCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGa           <  8:202148/149‑1 (MQ=255)                                                                                                                                    gaggagCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGa           <  3:123378/149‑1 (MQ=255)                                                                                                                                      ccagCACCTCCCCGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATg         >  6:61014/3‑149 (MQ=255)                                                                                                                                      ccagCACCTCCCCGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATg         >  2:140269/3‑149 (MQ=255)                                                                                                                                       cagCACCTCCCCGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCt                                                 >  6:217989/2‑108 (MQ=255)                                                                                                                                       cagCACCTCCCCGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCt                                                 <  5:217989/107‑1 (MQ=255)                                                                                                                                           aCCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATGGCCAg    >  5:278471/1‑149 (MQ=255)                                                                                                                                             cTCGGGGAGGTGCTGGGCGGGCGCTACCGGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATGGCCAgct  >  1:197101/1‑148 (MQ=255)                                                                                                                                             cTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAg                  >  7:45825/1‑133 (MQ=255)                                                                                                                                             cTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAg                  <  8:45825/133‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                TCGAACAGGCCACACCGCTGCGCGCCGCTGTACTGACGCGCGATGGCCTGTGGCACGTCGGTTCGACCCTGATCGATGCGGCCGGCGGCGGTTGTACCGCACCCAGCGTGGTGCGCACCCAGCCCGGCTGGGAGGAGCACCTCGGGGAGGTGCTGGGCGGGCGCTACCCGCGTGGCGACAGCAGCCGCCTGCGCCTGCAGGTGGCCCACCCCATGGATAACGGCCTGGTCAGCGGCATTCCTGAGTTCTTTCTCAACCATGCCGAGTTACAAGGTGCAGATGGCCAGCG  >  NC_002947/3065407‑3065695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑