Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,074,847 T→C 8.0% L151L (CTT→CTC)  PP_2684 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,074,8470TC 8.0% 139.2 / 2.1 50L151L (CTT→CTC) PP_2684membrane protein
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base C (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

TTGGTATCGATTACCTTGAAAAAGGCATGAGTTATTTTGCCAATCCCAAACTTCACGCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTGGAAGTATTCGTTGACCGAGTTGCTGTTGGCCTATCCGGTG  >  NC_002947/3074699‑3074991
                                                                                                                                                    |                                                                                                                                                
ttGGTATCGATTACCTTGAAAAAGGCATGAGTTATTTTGCCAATCCCAAACTTCACGCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCtt                                                                                                                                                  <  8:246984/149‑1 (MQ=255)
 tGGTATCGATTACCTTGAAAAAGGCATGAGTTATTTTGCCAATCCCAAACTTCACGCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCtt                                                                                                                                                  >  5:46618/1‑148 (MQ=255)
     aTCGATTACCTTGAAAAAGGCATGAGTTATTTTGCCGATCCCAAACTTCACGCCAAAGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCt                                                                                                                                             >  1:95414/1‑149 (MQ=255)
                              gTTATTTTGCCAATCCCAAACTTCACGCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGc                                                                                                                     <  2:224525/148‑1 (MQ=255)
                                 aTTTTGCCAATCCCAAACTTCACGCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCgttg                                                                                                                 >  8:63865/1‑149 (MQ=255)
                                   tttGCCAATCCCAAACTTCACGCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGa                                                                                                                            >  8:125041/1‑136 (MQ=255)
                                   tttGCCAATCCCAAACTTCACGCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGa                                                                                                                            <  7:125041/136‑1 (MQ=255)
                                              cAAACTTCACGCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCAc                                                                                                    >  2:95368/1‑149 (MQ=255)
                                               aaaCTTCACTCAAAGGTCATTATTTTCTTGCTCCTGACCTTCCACGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACt                                                                                                   <  6:165801/149‑1 (MQ=255)
                                                      aCGCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGgt                                                                                            <  6:145997/149‑1 (MQ=255)
                                                       cGCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGgtg                                                                                           <  6:46618/149‑1 (MQ=255)
                                                       cGCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTCGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGgtg                                                                                           >  1:223427/1‑149 (MQ=255)
                                                        gCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGATTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGgtgt                                                                                          >  7:178051/1‑149 (MQ=255)
                                                              gTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTg                                                                                    <  1:95368/149‑1 (MQ=255)
                                                                 attattGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGt                                                                                  <  3:64773/148‑1 (MQ=255)
                                                                   tattGTCTTGCTCCTGACCTTCAATGGCATTTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAg                                                                                              <  5:281252/134‑1 (MQ=255)
                                                                   tattGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAg                                                                                              >  6:281252/1‑134 (MQ=255)
                                                                       gTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATg                                                                            >  1:3286/1‑148 (MQ=255)
                                                                        tCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCgtt                                                                                                                  <  7:289333/109‑1 (MQ=255)
                                                                        tCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCgtt                                                                                                                  >  8:289333/1‑109 (MQ=255)
                                                                          ttGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGc                                                                        >  4:40364/1‑149 (MQ=255)
                                                                           tGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGc                                                                        >  6:66987/1‑148 (MQ=255)
                                                                              tCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTCGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTg                                                                                                          >  2:134893/1‑111 (MQ=255)
                                                                              tCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTCGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTg                                                                                                          <  1:134893/111‑1 (MQ=255)
                                                                                  gACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTgg                                                                >  3:21335/1‑149 (MQ=255)
                                                                                   aCCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTgg                                                                <  5:66987/148‑1 (MQ=255)
                                                                                    ccTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGc                                                              <  2:3286/149‑1 (MQ=255)
                                                                                     cTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCg                                                             >  1:338482/1‑149 (MQ=255)
                                                                                        cAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCgtc                                                           <  4:21335/148‑1 (MQ=255)
                                                                                           tGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAg                                                                         <  4:257229/131‑1 (MQ=255)
                                                                                           tGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAg                                                                         >  3:257229/1‑131 (MQ=255)
                                                                                                  ttgttgCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACt                                                <  7:63865/149‑1 (MQ=255)
                                                                                                        cATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGc                                                              <  8:79936/129‑1 (MQ=255)
                                                                                                        cATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGc                                                              >  7:79936/1‑129 (MQ=255)
                                                                                                        cATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGc                                                              <  2:272877/129‑1 (MQ=255)
                                                                                                        cATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGc                                                              >  1:272877/1‑129 (MQ=255)
                                                                                                         aTCGTTATGTATTTCCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTgg                                         <  3:40364/149‑1 (MQ=255)
                                                                                                         aTCGTTATGTATTACCTGCCTTAATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTg                                          >  4:3544/1‑148 (MQ=255)
                                                                                                          tCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTGGa                                        <  2:95414/149‑1 (MQ=255)
                                                                                                            gTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTCGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTGGaa                                       <  2:223427/148‑1 (MQ=255)
                                                                                                             ttATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTGGAAGt                                     >  8:238834/1‑149 (MQ=255)
                                                                                                                 gTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGc                                                    >  8:60739/1‑130 (MQ=255)
                                                                                                                 gTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGc                                                    <  7:60739/130‑1 (MQ=255)
                                                                                                                  tATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTGGAAGTATTc                                 <  3:3544/148‑1 (MQ=255)
                                                                                                                  tATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCAACTGGCCTGGAAGTATTCg                                >  7:137406/1‑149 (MQ=255)
                                                                                                                        cTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTGGAAGTATTCGTTGAcc                          >  7:235002/1‑149 (MQ=255)
                                                                                                                            cTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTGGAAGTATTCGTTGACCGAGt                      >  1:268272/1‑149 (MQ=255)
                                                                                                                            cTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTGGAAGTATTCGTTGACCGAGt                      >  1:227465/1‑149 (MQ=255)
                                                                                                                             ttGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTGGAAGTATTCGTTGACCGAGtt                     >  8:276895/1‑149 (MQ=255)
                                                                                                                               gATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTGGAAGTATTCGTTGACCGAGtt                     <  7:238834/147‑1 (MQ=255)
                                                                                                                                    aGGCCGGTTCTTTGCTTGACCTTAATCTAGGCGTCCGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTTGGCGGCGGTCGGCCACTGGCCTGGAAGTATTCGTTGACCGAGTTGCTGTTg              <  8:137406/149‑1 (MQ=255)
                                                                                                                                                 gCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTGGAAGTATTCGTTGACCGAGTTGCTGTTGGCCTATCCGGtg  <  2:268272/148‑1 (MQ=255)
                                                                                                                                                    tGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCAc                                                 >  3:132750/1‑98 (MQ=255)
                                                                                                                                                    tGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCAc                                                 <  4:132750/98‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
TTGGTATCGATTACCTTGAAAAAGGCATGAGTTATTTTGCCAATCCCAAACTTCACGCCAAGGTCATTATTGTCTTGCTCCTGACCTTCAATGGCATGTTGTTGCATCGTTATGTATTACCTGCCTTGATCAAGGCCGGTTCTTTGCTTGACCTGAATATAGGCGTACGGATGTTTGCGTTGTTTTCTGGTGCACTGTCAGGTGTTTCCTGGTTATATGCAGCGATGCTGGGCGTCGGTCGGCCACTGGCCTGGAAGTATTCGTTGACCGAGTTGCTGTTGGCCTATCCGGTG  >  NC_002947/3074699‑3074991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑