Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,121,594 C→G 11.0% G90A (GGC→GCC)  PP_2738 ← putative Transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,121,5940CG11.0% 121.5 / 5.5 44G90A (GGC→GCC) PP_2738putative Transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (19/20);  new base G (2/3);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGTGGCGTTGGCGTACAACTGCTCGAAGTAGGCACGCAGGGCCTGCAGCCCCTG  >  NC_002947/3121451‑3121742
                                                                                                                                               |                                                                                                                                                    
tgcctTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGc                                                                                                                                                  >  4:64939/1‑148 (MQ=255)
    tttGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGACCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTCCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAgcc                                                                                                                                             >  3:302103/1‑147 (MQ=255)
        aGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCgg                                                                                                                                         <  3:64939/149‑1 (MQ=255)
        aGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGCCTGGCCGGCGGCCAGccgatc                                                                                                                                         <  2:169993/149‑4 (MQ=255)
          ccAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGGCGGCCAGccgatc                                                                                                                                         >  2:281055/1‑144 (MQ=255)
          ccAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGt                                                                                                                                       <  8:157034/149‑1 (MQ=255)
          ccAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGt                                                                                                                                       <  7:124972/149‑1 (MQ=255)
          ccAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGCGCCCGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGGCGGCCAGccgatc                                                                                                                                         <  1:281055/147‑4 (MQ=255)
             gCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTgg                                                                                                                                     >  4:196951/1‑148 (MQ=255)
                      tgtgCCCATGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAAAAGTCCTGGTGGAAATGCACACGGTCGCGCCATAGCAGGCAGCTGCAGCCGTGCAGGGTGATCGGCTGGCCGCCCGCCAGGCCCGGGTGGCaaacagg                                                                                                                             >  2:266343/1‑143 (MQ=255)
                           ccATGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTc                                                                                                                      <  3:196951/149‑1 (MQ=255)
                              tGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCaa                                                                                                                   >  8:45569/1‑149 (MQ=255)
                              tGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCaa                                                                                                                   <  4:118591/149‑1 (MQ=255)
                                   ggATGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAg                                                                                                              <  7:272708/149‑1 (MQ=255)
                                        gTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTAGCAAAACTGCAGTGTCCAACGCAgg                                                                                                             <  4:213854/145‑1 (MQ=255)
                                        gTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTAGCAAAACTGCAGTGTCCAACGCAgg                                                                                                             >  3:213854/1‑145 (MQ=255)
                                         tGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGcc                                                                                                        >  6:279243/1‑149 (MQ=255)
                                            tCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTg                                                                                                     >  2:310592/1‑149 (MQ=255)
                                              aTAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGcc                                                                                                   >  5:160119/1‑149 (MQ=255)
                                              aTAAAGCAGCGCGCCGGCGTCGAAATAGTGCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCTACGCAGGTAGCCCTGc                                                                                                    <  4:302103/148‑1 (MQ=255)
                                               tAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGccc                                                                                                  >  5:173073/1‑149 (MQ=255)
                                                 aaGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCgg                                                                                                <  7:45569/149‑1 (MQ=255)
                                                 aaGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCACgt                                                                                                >  7:179074/1‑148 (MQ=255)
                                                  aGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCggg                                                                                               <  2:28377/149‑1 (MQ=255)
                                                      gggCGCCGGCGTCGAATTTTTCCTGGTGGAAATGCACCCGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCTGCTGGCCGGCGTCCAGGCGCGGGTGGGAAAACTGCAGTGTCGAACGGAGGTAGCCCTGCCCGGGCttt                                                                                           <  5:292098/149‑1 (MQ=255)
                                                             ggCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGc                                                                                              <  4:212990/139‑1 (MQ=255)
                                                             ggCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGc                                                                                              >  3:212990/1‑139 (MQ=255)
                                                                gTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCgg                                                                                 >  3:161385/1‑149 (MQ=255)
                                                                     aaTAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTg                                                                            <  8:185637/149‑1 (MQ=255)
                                                                     aaTAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTg                                                                            <  4:161385/149‑1 (MQ=255)
                                                                        aGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTAc                                                                                         >  4:285046/1‑133 (MQ=255)
                                                                        aGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTAc                                                                                         <  3:285046/133‑1 (MQ=255)
                                                                            cTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAgg                                                                     >  2:262455/1‑149 (MQ=255)
                                                                                tggAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTa                                                                 <  7:277123/149‑1 (MQ=255)
                                                                                                 gcgcCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGtggcg                                                 <  6:173073/148‑1 (MQ=255)
                                                                                                    ccAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCTCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGtggcgttgg                                             <  1:262455/149‑1 (MQ=255)
                                                                                                          caggcagCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGTGGCGTTGGCGTAc                                        >  1:194454/1‑148 (MQ=255)
                                                                                                                  tGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGTGGCGTTGGCGTACAACTGc                                  <  6:265248/146‑1 (MQ=255)
                                                                                                                  tGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGTGGCGTTGGCGTACAACTGc                                  >  5:265248/1‑146 (MQ=255)
                                                                                                                    cAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGTGGCGTTGGCGTACAACTGCTCGaa                             <  1:310592/149‑1 (MQ=255)
                                                                                                                            cAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGTGGCGTTGGCGTACAACTGCTCGAAGTAGGCAc                     >  2:188885/1‑149 (MQ=255)
                                                                                                                                    tCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGTGGCGTTGGCGTACAACTGCTCGAAGTAGGCACGCAGGGcc             >  3:117048/1‑149 (MQ=255)
                                                                                                                                    gcgccTGGCCGGCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGAAATCGGTGGCGTTGGCGTACAACTGCTCGAAGTAGGCACGCAGGGcc             >  8:170463/5‑149 (MQ=255)
                                                                                                                                      ggCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGTGGCGTTGGCGTACAACTGCTCGAAGTAGGCACGCAGGGCCt            <  6:160119/148‑1 (MQ=255)
                                                                                                                                            ccgccgGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGTGGCGTTGGCGTACAACTGCTCGAAGTAGGCACGCAGGGCCTGCAGccc     >  1:96899/1‑149 (MQ=255)
                                                                                                                                             cgccgGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGTGGCGTTGGCGTACAACTGCTCGAAGTAGGCACGCAGGGCCTGCAGcccc    <  4:117048/149‑1 (MQ=255)
                                                                                                                                               ccgGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCCCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGTGGCGTTGGCGTACAACTGCTCGAAGTAGGCACGCAGGGCCTGCAGCCCCTg  >  5:10752/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TGCCTTTGAGCCAGCGGATTGCTGTGCCCATGACGGGAAGGTGCTCATAAAGCAGGGCGCCGGCGTCGAAATAGTCCTGGTGGAAATGCACACGGTCGCGCCAATGCAGGCAGCTGCAGCCTTGCAGCGTGATCGGCTGGCCGCCGGCCAGGCGCGGGTGGCAAAACTGCAGTGTCCAACGCAGGTAGCCCTGCCCGGGCTTTACCTCGTCGGCGCTGGTGAAGGCGTAGCGGATATCGGTGGCGTTGGCGTACAACTGCTCGAAGTAGGCACGCAGGGCCTGCAGCCCCTG  >  NC_002947/3121451‑3121742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑